Tryptone
Based on 1 Customer Validation
Tryptone is a peptide-rich bacterial culture medium component that can regulate bacterial cell surface proteins and biofilm-related genes. Tryptone promotes the expression and assembly of bacterial adhesion proteins (such as LapA and LapF) by providing peptide substances as structural factors, enhancing cell surface hydrophobicity and intercellular adhesion, thereby stabilizing the biofilm matrix and supporting the maturation and maintenance of bacterial biofilms. The peptide mixture contained in Tryptone can specifically regulate the transcription of bacterial adhesion-related genes (such as activating LapA and inhibiting LapF), affecting the synthesis and localization of biofilm structural proteins.
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- CAS No.: 73049-73-7
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Stockage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Tryptone (10 g/L; 10 min; 37°C) significantly improved the capture efficiency of T4 phage conjugated magnetic particles for Escherichia coli, and this effect was temperature-dependent[1].
In the Pseudomonas biofilm formation experiment, Tryptone (10 g/L; 24 h) promoted the formation of mature biofilms of wild-type and Fis-overexpressing strains of Pseudomonas putida, increasing the amount of biofilm by 1.3-2.9 times compared with the M9 medium group without Tryptone, and was related to peptide-mediated cell surface protein regulation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Escherichia coli (ATCC 11303)
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Concentration:10 g/L (added to Luria-Bertani medium or phosphate buffer)
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Incubation Time:10 min at 37°C
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Result:Increased the capture efficiency of T4 bacteriophage-conjugated Fe3O4 magnetic particles, with viable bacterial counts in the supernatant decreasing by 70% compared to Tryptone-free conditions.
Bacterial capture was temperature-dependent, with no significant effect observed at 4°C. Flow cytometry and plate counting assays showed that Tryptone facilitated irreversible binding between T4 bacteriophage and E. coli surface receptors, enhancing magnetic particle-mediated isolation of bacteria.
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Cell Line:Pseudomonas putida strains PSm (wild type) and F15 (fis-overexpression)
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Concentration:10 g/L (supplemented to M9-0.2CAA medium)
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Incubation Time:24 h
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Result:Increased biofilm formation measured by crystal violet staining, with wild-type PSm showing a 1.3-fold increase and F15 strain exhibiting a 2.9-fold increase compared to Tryptone-free M9 medium.
qPCR analysis revealed upregulated expression of adhesion genes lapA and downregulated lapF in F15 cells, while Western blot confirmed enhanced Fis protein levels in Tryptone-supplemented cultures.
Biofilm matrix stability was assessed via protease K treatment, showing reduced dispersion in Tryptone-containing media, indicating polypeptide-mediated reinforcement of extracellular matrix structure.
Chemical Information
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CAS No. 73049-73-7
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Tryptone]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvant et solubilité
H2O : 100 mg/mL (Need ultrasonic)
Pureté et documentation
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Fiche technique (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Liana AE, et al. T4 bacteriophage conjugated magnetic particles for E. coli capturing: Influence of bacteriophage loading, temperature and tryptone. Colloids Surf B Biointerfaces. 2017 Mar 1;151:47-57. [Content Brief]
[2]. Puhm M, et al. Tryptone in Growth Media Enhances Pseudomonas putida Biofilm. Microorganisms. 2022 Mar 14;10(3):618. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Tryptone Related Classifications
HY-W133898 Related Classifications
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