Chitotriose trihydrochloride
Based on 1 Customer Validation
Chitotriose trihydrochloride is an orally active chitooligosaccharide with antioxidant activities. Chitotriose trihydrochloride inhibits hydroxylation of benzoate to salicylate by H2O2 in the presence of Cu2+ (IC50 value of 80 μM).
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- Pureza : 99.94%
- No. CAS: 117436-78-9
- Fòrmula: C18H38Cl3N3O13
- Peso molecular:610.87
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Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Chemical Information
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No. CAS 117436-78-9
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Appearance Solid
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Peso molecular 610.87
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Fòrmula C18H38Cl3N3O13
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Color White to off-white
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SMILES
OC[C@@H](O[C@H]1O[C@H]([C@H](O)CO)[C@H](O)[C@@H](N)C=O)[C@H]([C@@H]([C@H]1N)O)O[C@@H]([C@@H]([C@H]2O)N)O[C@@H]([C@H]2O)CO.Cl.Cl.Cl
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Structure Classification
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Initial Source
Bacillus sp.
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (163.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (40.93 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.6370 mL | 8.1850 mL | 16.3701 mL | 40.9252 mL |
| 5 mM | 0.3274 mL | 1.6370 mL | 3.2740 mL | 8.1850 mL | |
| 10 mM | 0.1637 mL | 0.8185 mL | 1.6370 mL | 4.0925 mL | |
| 15 mM | 0.1091 mL | 0.5457 mL | 1.0913 mL | 2.7283 mL | |
| 20 mM | 0.0819 mL | 0.4093 mL | 0.8185 mL | 2.0463 mL | |
| 25 mM | 0.0655 mL | 0.3274 mL | 0.6548 mL | 1.6370 mL | |
| 30 mM | 0.0546 mL | 0.2728 mL | 0.5457 mL | 1.3642 mL | |
| 40 mM | 0.0409 mL | 0.2046 mL | 0.4093 mL | 1.0231 mL | |
| DMSO | 50 mM | 0.0327 mL | 0.1637 mL | 0.3274 mL | 0.8185 mL |
| 60 mM | 0.0273 mL | 0.1364 mL | 0.2728 mL | 0.6821 mL | |
| 80 mM | 0.0205 mL | 0.1023 mL | 0.2046 mL | 0.5116 mL | |
| 100 mM | 0.0164 mL | 0.0819 mL | 0.1637 mL | 0.4093 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Chitotriose trihydrochloride Related Classifications
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