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Cat. No.: HY-184448

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CIB-Q22

Name: CIB-Q22
Brand: MedChemExpress
SKU: HY-184448
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CIB-Q22 is a potent pyruvate carboxylase (PC) inhibitor with an IC₅₀ of 1.74 nM. CIB-Q22 suppresses hepatocellular carcinoma (HCC) cell proliferation, migration, and invasion. CIB-Q22 induces apoptosis and ferroptosis, promotes mitochondrial oxidative stress and dysfunction, inhibits glycolysis and anaplerotic flux, and reverses epithelial-mesenchymal transition (EMT). CIB-Q22 can be used for the study of hepatocellular carcinoma.

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CAS No.: 2963747-43-3
Formule: C₁₈H₂₂FNO₄
Masse moléculaire:335.37

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

CIB-Q22 Chemical Structure

CAS No.: 2963747-43-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Parmacokinetics
- In Vivo
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
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Activité biologique

Description

IC₅₀ & Target^[1]

Bax

Bcl-2

Caspase 3

GPX4

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GPX1
GPX4

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC50	213.5 nM	Exhibits potent cytotoxicity against A549 cells for 48 h. Exhibits potent cytotoxicity against A549 cells for 48 h.	42286802
Huh-7	IC50	78.86 nM	Exhibits potent cytotoxicity against Huh-7 cells for 48 h. Exhibits potent cytotoxicity against Huh-7 cells for 48 h.	42286802
MHCC97L	IC50	153.3 nM	Exhibits potent cytotoxicity against MHCC97L cells for 48h. Exhibits potent cytotoxicity against MHCC97L cells for 48h.	42286802
HCCLM3	IC50	25.18 nM	Exhibits potent cytotoxicity against HCCLM3 cells for 48 h. Exhibits potent cytotoxicity against HCCLM3 cells for 48 h.	42286802

In Vitro

CIB-Q22 (48 h) exhibits potent cytotoxicity against HCCLM3, Huh-7, MHCC97L, and A549 cells, with IC₅₀ values of 25.18 nM, 78.86 nM, 153.3 nM, and 213.5 nM, respectively^[1].
CIB-Q22 (48 h) shows minimal cytotoxicity against SK-Hep-1 cells, which express low levels of PC, as well as against normal RPE-1 cells^[1].
CIB-Q22 shows no significant cardiotoxicity in a hERG potassium channel inhibition assay compared to the positive control Terfenadine (HY-B1193)^[1].
CIB-Q22 (10-40 nM; 21 days) suppresses the proliferative capacity of HCCLM3 cells ^[1].
CIB-Q22 (2-10 nM; 24 h) decreases the migration and invasion of HCCLM3 cells, upregulates E-cadherin, and downregulates N-cadherin and fibronectin^[1].
CIB-Q22 (40-160 nM; 48 h) induces apoptosis in HCCLM3 cells^[1].
CIB-Q22 (50-1600 nM; 52 °C) stabilizes PC thermal stability in HCCLM3 cells in a dose-dependent manner^[1].
CIB-Q22 (40-160 nM; 0-48 h) activates Caspase-3 and induces PARP cleavage in HCCLM3 cells in time- and concentration-dependent manners^[1].
CIB-Q22 (40-160 nM; 48 h) induces mitochondrial dysfunction in HCCLM3 cells^[1].
CIB-Q22 (40-160 nM; 48 h) increases the levels of lipid peroxidation in HCCLM3 cells^[1].
CIB-Q22 (40-160 nM; 48 h) induces ferroptosis in HCC cells in a PC-dependent manner^[1].
CIB-Q22 (40 nM; 1-6 h) induces a significant increase in intracellular ROS production in HCCLM3 cells^[1].
CIB-Q22 (40 nM; 24 h) induces oxidative stress in HCCLM3 cells, as evidenced by reduced NADH/NAD⁺ and GSH/GSSG ratios, decreased ATP levels, and suppressed glucose consumption and lactate production^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HCCLM3 cells
Concentration:

2, 5, or 10 nM
Incubation Time:

24 h
Result:

Upregulated E-cadherin and downregulated N-cadherin and fibronectin in HCCLM3 cells.

Western Blot Analysis^[1]

Cell Line:

HCCLM3 cells
Concentration:

40, 80, or 160 nM
Incubation Time:

0, 2, 4, 12, 24 or 48 h
Result:

Activated Caspase-3 and induced PARP cleavage in a time-dependent manner (160 nM; (0, 2, 4, 12, 24 or 48 h). Activated Caspase-3 and induced PARP cleavage in a concentration-dependent manner (40, 80, or 160 nM; 48 h).

Western Blot Analysis^[1]

Cell Line:

HCCLM3 cells
Concentration:

40, 80, or 160 nM
Incubation Time:

48 h
Result:

Downregulated GPX4 and SLC7A11/xCT expression; induced ferroptosis in a PC-dependent manner.

Cell Proliferation Assay^[1]

Cell Line:

HCCLM3 cells
Concentration:

10, 20, or 40 nM
Incubation Time:

21 days
Result:

Suppressed the proliferative capacity of HCCLM3 cells in a concentration-dependent manner.

Cell Invasion Assay^[1]

Cell Line:

HCCLM3 cells
Concentration:

2, 5, or 10 nM
Incubation Time:

24 h
Result:

Decreased the number of invading HCCLM3 cells.

Apoptosis Analysis^[1]

Cell Line:

HCCLM3 cells
Concentration:

40, 80, or 160 nM
Incubation Time:

48 h
Result:

Induced apoptosis in HCCLM3 cells in a concentration-dependent manner.

Immunofluorescence^[1]

Cell Line:

HCCLM3 cells
Concentration:

40 nM
Incubation Time:

0, 1, 3, or 6 h
Result:

Induced a significant increase in intracellular ROS production.

Parmacokinetics

Species	Dose	Route	T_1/2	T_max	C_max	AUC_0-∞	AUC_0-t	MRT_0-t	MRT_0-∞	C₀	V_ss	CL	V_z	F
Mice^[1]	10 mg/kg	i.v.	1.21 h	0.083 h	5793.9 ng/mL	3062.96 ng·h/mL	3073.17 ng·h/mL	0.69 h	0.72 h	8129.43 ng/mL	239.02 mL/kg	337.77 mL/h/kg	599.15 mL/kg	/
Mice^[1]	25 mg/kg	p.o.	1.79 h	0.33 h	508.67 ng/mL	1060.10 ng·h/mL	1069.27 ng·h/mL	2.49 h	2.62 h	/	/	/	/	13.84 %

In Vivo

CIB-Q22 (15-50 mg/kg ; i.p.; every 2 days; 21 days) suppresses tumor growth in an orthotopic HCC mouse model (MHCC97L-luciferase cells in SCID mice) without causing significant body weight changes, exhibiting comparable antitumor efficacy but improved safety compared to Sorafenib (HY-10201) ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Five-week-old male SCID mice are orthotopically injected with 50 μL of 1 × 10⁶ MHCC97L-luciferase cells into the left liver lobe ^[1]
Dosage:

15 mg/kg, 30 mg/kg, 50 mg/kg
Administration:

Intraperitoneal injection (i.p.); every 2 days; 21 days (starting 7 days after tumor implantation)
Result:

Significantly inhibited tumor growth in a dose-dependent manner. Did not cause significant body weight changes at any dose, indicating minimal systemic toxicity. H&E staining of major organs revealed no evident pathological abnormalities, suggesting favorable long-term safety. Immunohistochemical (IHC) analysis of tumor tissues showed significantly reduced GPX4 expression and increased 4-HNE level, a key marker of ferroptosis. TUNEL staining demonstrated increased apoptotic cell death within tumor tissues.

Chemical Information

CAS No. 2963747-43-3
Masse moléculaire 335.37
Formule C₁₈H₂₂FNO₄
SMILES

NC1=C(F)C(CCC2=CC(OC)=C(OC)C(OC)=C2)=CC=C1OC
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Qiao Z, et al. Design, Synthesis, and Evaluation of Bibenzyl Analogues against Hepatocellular Carcinoma by Targeting Pyruvate Carboxylase. J. Med. Chem. 2026 Jun 25;69(12):14333-14364. [Content Brief]

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Activité biologique
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- In Vitro
- In Vivo
Chemical Information
Pureté et documentation
Références

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CIB-Q22

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Chemical Information

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