1. GPCR/G Protein
    Neuronal Signaling
    Metabolic Enzyme/Protease
  2. mAChR
    Dopamine Receptor
    Drug Metabolite
  3. Clozapine N-oxide dihydrochloride

Clozapine N-oxide dihydrochloride 

Cat. No.: HY-17366A Purity: 98.17%
Handling Instructions

Clozapine N-oxide dihydrochloride is a major metabolite of Clozapine and a human muscarinic designer receptors (DREADDs) agonist. Clozapine N-oxide dihydrochloride specifically activates the DREADD receptor hM3Dq. Clozapine N-oxide dihydrochloride can cross the blood-brain barrier. Clozapine is a potent dopamine antagonist and also a potent and selective muscarinic M4 receptor (EC50=11 nM) agonist.

For research use only. We do not sell to patients.

Clozapine N-oxide dihydrochloride Chemical Structure

Clozapine N-oxide dihydrochloride Chemical Structure

CAS No. : 2250025-93-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 83 In-stock
Estimated Time of Arrival: December 31
5 mg USD 75 In-stock
Estimated Time of Arrival: December 31
10 mg USD 110 In-stock
Estimated Time of Arrival: December 31
50 mg USD 420 In-stock
Estimated Time of Arrival: December 31
100 mg USD 650 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of Clozapine N-oxide dihydrochloride:

Top Publications Citing Use of Products

    Clozapine N-oxide dihydrochloride purchased from MCE. Usage Cited in: Biol Psychiatry. 2017 May 1;81(9):737-747.

    c-fos immunoreactivity in the VTA 90 minutes after injections of 1 mg/kg Clozapine N-oxide (CNO).
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Clozapine N-oxide dihydrochloride is a major metabolite of Clozapine and a human muscarinic designer receptors (DREADDs) agonist. Clozapine N-oxide dihydrochloride specifically activates the DREADD receptor hM3Dq. Clozapine N-oxide dihydrochloride can cross the blood-brain barrier[1][2][3]. Clozapine is a potent dopamine antagonist and also a potent and selective muscarinic M4 receptor (EC50=11 nM) agonist[4][5].

    IC50 & Target

    Human muscarinic designer receptors (DREADDs)[1]

    In Vitro

    Clozapine N-oxide (CNO) can bind to non-DREADD receptors at concentrations required for DREADD activation, and undergoes reverse-metabolism to its parent compound clozapine, an atypical antipsychotic that acts at a variety of pharmacological targets and produces numerous physiological and behavioral effects[2].

    In Vivo

    After a single intraperitoneal (i.p.) injection of Clozapine N-oxide (1 mg/kg) into mice, Clozapine N-oxide (CNO) plasma levels peak at 15 min and are very low after 2 h. Despite the short plasma half-life of CNO in mice, the biological effects that have been described after acute treatment of DREADD-expressing experimental animals are usually much longer (6-10 h)[1].

    Molecular Weight

    415.74

    Formula

    C₁₈H₂₁Cl₃N₄O

    CAS No.

    2250025-93-3

    SMILES

    C[N+]1([O-])CCN(C2=NC3=CC(Cl)=CC=C3NC4=CC=CC=C42)CC1.[H]Cl.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 300 mg/mL (721.60 mM; Need ultrasonic)

    H2O : 100 mg/mL (240.53 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4053 mL 12.0267 mL 24.0535 mL
    5 mM 0.4811 mL 2.4053 mL 4.8107 mL
    10 mM 0.2405 mL 1.2027 mL 2.4053 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 7.5 mg/mL (18.04 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 7.5 mg/mL (18.04 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Clozapine N-oxidemAChRDopamine ReceptorDrug MetaboliteMuscarinic acetylcholine receptorhM3DqBBBDREADDsClozapinemetaboliteG-proteinmuscarinic-M4Inhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email address *

    Phone number *

     

    Organization name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Clozapine N-oxide dihydrochloride
    Cat. No.:
    HY-17366A
    Quantity:
    MCE Japan Authorized Agent: