VU0514009
Based on 1 Customer Validation
VU0514009 is a competitive chemokine-like receptor 1 (CMKLR1) antagonist (EC50=2 nM). VU0514009 prevents chemerin-9-induced arrestin recruitment and receptor internalization, significantly reducing Ca2+ flux response in HEK293 cells. VU0514009 is promising for research of inflammatory diseases and metabolic syndrome.
For research use only. We do not sell to patients.
- Purity: 99.53%
- CAS No.: 932973-77-8
- Formula: C21H20ClN3O5S2
- Molecular Weight:493.98
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Chemerin Receptor Isoforms
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Biological Activity
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ChemR23/CMKLR1 2 nM (EC50) |
Chemical Information
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CAS No. 932973-77-8
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Appearance Solid
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Molecular Weight 493.98
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Formula C21H20ClN3O5S2
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Color White to light yellow
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SMILES
O=C1C(S(C2=CC=C(C(Cl)=C2)C)(=O)=O)=CNC(SCC(NC3=CC(C)=CC=C3OC)=O)=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (101.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (10.12 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0244 mL | 10.1219 mL | 20.2437 mL | 50.6093 mL |
| 5 mM | 0.4049 mL | 2.0244 mL | 4.0487 mL | 10.1219 mL | |
| 10 mM | 0.2024 mL | 1.0122 mL | 2.0244 mL | 5.0609 mL | |
| 15 mM | 0.1350 mL | 0.6748 mL | 1.3496 mL | 3.3740 mL | |
| 20 mM | 0.1012 mL | 0.5061 mL | 1.0122 mL | 2.5305 mL | |
| 25 mM | 0.0810 mL | 0.4049 mL | 0.8097 mL | 2.0244 mL | |
| 30 mM | 0.0675 mL | 0.3374 mL | 0.6748 mL | 1.6870 mL | |
| 40 mM | 0.0506 mL | 0.2530 mL | 0.5061 mL | 1.2652 mL | |
| 50 mM | 0.0405 mL | 0.2024 mL | 0.4049 mL | 1.0122 mL | |
| 60 mM | 0.0337 mL | 0.1687 mL | 0.3374 mL | 0.8435 mL | |
| 80 mM | 0.0253 mL | 0.1265 mL | 0.2530 mL | 0.6326 mL | |
| 100 mM | 0.0202 mL | 0.1012 mL | 0.2024 mL | 0.5061 mL |
VU0514009 Related Classifications
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