Coumarin 6H
Based on 1 Customer Validation
Coumarin 6H, a Coumarin (HY-N0709) derivative, is a laser dye. The fluorescence emission of Coumarin 6H can be enhanced by hydrogen bonding interactions.
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- Pureza : 99.05%
- No. CAS: 58336-35-9
- Fòrmula: C15H15NO2
- Peso molecular:241.29
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Actividad biológica
Descripciòn
In Vitro
The Coumarin 6H with twistblocked and stronger electron-donating julolidine group exhibits higher quantum yield, better biocompatibility, as well as less background fluorescence when suppressed by a fluorescence quenching group. Thus, the Coumarin 6H in probe LH-1 may help to reduce the background fluorescence. In probe LH-1, the fluorescence of Coumarin 6H fluorophore not only can be quenched by the rotation of N-N group, but also can be quenched by the 2,4-dinitrobenzenze group via donor-excited photoinduced electron transfer (d-PET) mechanism[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Emission (Em)
454
Excitation (Ex)
344
Chemical Information
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No. CAS 58336-35-9
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Appearance Solid
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Peso molecular 241.29
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Fòrmula C15H15NO2
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Color Yellow to brown
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SMILES
O=C1C=CC2=CC3=C4N(CCCC4=C2O1)CCC3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
In Vitro:
DMSO : 12.5 mg/mL (51.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. P M French, et al. Passively mode-locked cw Coumarin 6 ring dye laser. Opt Lett. 1989 Feb 15;14(4):217-8. [Content Brief]
[2]. Zhixiang Han, et al. A novel fluorescent probe with extremely low background fluorescence for sensing hypochlorite in zebrafish. Anal Biochem. 2020 Aug 1;602:113795. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1444 mL | 20.7220 mL | 41.4439 mL | 103.6098 mL |
| 5 mM | 0.8289 mL | 4.1444 mL | 8.2888 mL | 20.7220 mL | |
| 10 mM | 0.4144 mL | 2.0722 mL | 4.1444 mL | 10.3610 mL | |
| 15 mM | 0.2763 mL | 1.3815 mL | 2.7629 mL | 6.9073 mL | |
| 20 mM | 0.2072 mL | 1.0361 mL | 2.0722 mL | 5.1805 mL | |
| 25 mM | 0.1658 mL | 0.8289 mL | 1.6578 mL | 4.1444 mL | |
| 30 mM | 0.1381 mL | 0.6907 mL | 1.3815 mL | 3.4537 mL | |
| 40 mM | 0.1036 mL | 0.5180 mL | 1.0361 mL | 2.5902 mL | |
| 50 mM | 0.0829 mL | 0.4144 mL | 0.8289 mL | 2.0722 mL |