CSN5-IN-1
CSN5-IN-1 (compound Ac-11) is an inhibitor of CSN5, with IC50 values of 12.56 μM and 19 μM as measured by FP assay and fluorescence assay, respectively. CSN5-IN-1 can also downregulate the expression of PD-L1 and upregulate the expression of NEDD8-Cul1 in cells.
For research use only. We do not sell to patients.
- Formula: C17H22N2O2S
- Molecular Weight:318.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
IC50: 12.56 μM (CSN5; FP assay); 19 μM (CSN5; Fluor assay)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
24.1 μM
Compound: AC-11
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Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
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[PMID: 39129245] |
| HepG2 | IC50 |
39.14 μM
Compound: AC-11
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Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
|
[PMID: 39129245] |
| MDA-MB-231 | IC50 |
22.8 μM
Compound: AC-11
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Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation measured after 3 days by MTT assay
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[PMID: 39129245] |
Chemical Information
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Molecular Weight 318.43
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Formula C17H22N2O2S
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SMILES
O=C(C1=C(C(C)CC2=CC=C(C(C)(C)C)C=C2)SC(N)=N1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CSN5-IN-1 Related Classifications
HY-159601 Related Classifications
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