Cucurbitacin IIb
Based on 2 publication(s) in Google Scholar
Cucurbitacin IIb is an active component isolated from Hemsleya amabilis, induces apoptosis with anti-inflammatory activity. Cucurbitacin IIb inhibits phosphorylation of STAT3, JNK and Erk1/2, enhances the phosphorylation of IκB and NF-κB (p65), blocks nuclear translocation of NF-κB (p65) and decreases mRNA levels of IκBα and TNF-α.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 50298-90-3
- Formula: C30H48O7
- Molecular Weight:520.70
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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NF-κB Signaling Compound Library
HY-L014
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Natural Product Library
HY-L021
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Terpenoids Library
HY-L056
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Traditional Chinese Medicine Active Compound Library
HY-L065
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Anti-inflammatory Traditional Chinese Medicine Active Compound Library
HY-L114
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Plant-Sourced Natural Product Library
HY-L115
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Cancer Stem Cells Compound Library
HY-L135
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Pain-Related Compound Library
HY-L139
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Natural Product Library Plus
HY-L021P
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Natural Product and Natural Product-Like Compound Library
HY-L021M
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Bioactive Compound Library
HY-L001
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Miao Ethnicity Medicine Compound Library
HY-L190
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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High-Throughput Natural Product Library
HY-L206
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Natural Product Diversity Scaffold Library
HY-L245
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Mass Spectrometry Natural Product Library
HY-L262
Publications Citing Use of MedChemExpress (MCE) Cucurbitacin IIb
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COLO 205 | IC50 |
0.64 μg/mL
Compound: 5
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Cytotoxicity against human COLO205 cells after 48 hrs by MTT assay
Cytotoxicity against human COLO205 cells after 48 hrs by MTT assay
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[PMID: 24717151] |
| HepG2 2.2.15 | CC50 |
60.6 μM
Compound: 2
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Cytotoxicity against human HepG2.2.15 cells
Cytotoxicity against human HepG2.2.15 cells
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[PMID: 23385212] |
| HepG2 2.2.15 | IC50 |
>60.6 μM
Compound: 2
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Antiviral activity against HBV infected in human HepG2.2.15 cells assessed as inhibition of viral e antigen production
Antiviral activity against HBV infected in human HepG2.2.15 cells assessed as inhibition of viral e antigen production
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[PMID: 23385212] |
| HepG2 2.2.15 | IC50 |
>60.6 μM
Compound: 2
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Antiviral activity against HBV infected in human HepG2.2.15 cells assessed as inhibition of viral surface antigen production
Antiviral activity against HBV infected in human HepG2.2.15 cells assessed as inhibition of viral surface antigen production
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[PMID: 23385212] |
| HepG2 2.2.15 | IC50 |
31.4 μM
Compound: 2
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Antiviral activity against HBV infected in human HepG2.2.15 cells assessed as inhibition of viral DNA replication
Antiviral activity against HBV infected in human HepG2.2.15 cells assessed as inhibition of viral DNA replication
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[PMID: 23385212] |
| KB | ED50 |
>50 μg/mL
Compound: 2
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 6549470] |
| NCI-H460 | IC50 |
7.09 μg/mL
Compound: 5
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Cytotoxicity against human H460 cells after 48 hrs by MTT assay
Cytotoxicity against human H460 cells after 48 hrs by MTT assay
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[PMID: 24717151] |
| P388 | ED50 |
>50 μg/mL
Compound: 2
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Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
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[PMID: 6549470] |
| SW-620 | IC50 |
1.03 μg/mL
Compound: 5
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Cytotoxicity against human SW620 cells after 48 hrs by MTT assay
Cytotoxicity against human SW620 cells after 48 hrs by MTT assay
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[PMID: 24717151] |
Chemical Information
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CAS No. 50298-90-3
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Appearance Solid
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Molecular Weight 520.70
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Formula C30H48O7
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Color White to off-white
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SMILES
C[C@@]([C@@](CC=C1[C@@]2([H])C[C@H](O)[C@@H](O)C1(C)C)([H])[C@@]2(C)C3=O)(C[C@@H](O)[C@]4([H])[C@@](C)(O)C(CCC(C)(O)C)=O)[C@@]4(C3)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
Cucurbitacin IIb mitigates concanavalin A-induced acute liver injury by suppressing M1 macrophage polarization. [Abstract]2025 Jan 3:147:113964. PMID: 39755110 -
Int Immunopharmacol
Recombinant fibroblast growth factor 4 ameliorates axonal regeneration and functional recovery in acute spinal cord injury through altering microglia/macrophage phenotype. [Abstract]2024 May 9:134:112188. PMID: 38728880
Solvent & Solubility
DMSO : 100 mg/mL (192.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9205 mL | 9.6025 mL | 19.2049 mL | 48.0123 mL |
| 5 mM | 0.3841 mL | 1.9205 mL | 3.8410 mL | 9.6025 mL | |
| 10 mM | 0.1920 mL | 0.9602 mL | 1.9205 mL | 4.8012 mL | |
| 15 mM | 0.1280 mL | 0.6402 mL | 1.2803 mL | 3.2008 mL | |
| 20 mM | 0.0960 mL | 0.4801 mL | 0.9602 mL | 2.4006 mL | |
| 25 mM | 0.0768 mL | 0.3841 mL | 0.7682 mL | 1.9205 mL | |
| 30 mM | 0.0640 mL | 0.3201 mL | 0.6402 mL | 1.6004 mL | |
| 40 mM | 0.0480 mL | 0.2401 mL | 0.4801 mL | 1.2003 mL | |
| 50 mM | 0.0384 mL | 0.1920 mL | 0.3841 mL | 0.9602 mL | |
| 60 mM | 0.0320 mL | 0.1600 mL | 0.3201 mL | 0.8002 mL | |
| 80 mM | 0.0240 mL | 0.1200 mL | 0.2401 mL | 0.6002 mL | |
| 100 mM | 0.0192 mL | 0.0960 mL | 0.1920 mL | 0.4801 mL |
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