Cy3-PEG2-exo-BCN
Cy3-PEG2-exo-BCN is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) containing 2 PEG units. Cy3-PEG2-exo-BCN contains the lyophilic bidentate macrocyclic ligand BCN, which can further synthesize macrocyclic complexes. In click chemistry, exo-BCN can react with molecules containing azide groups to form stable triazoles in the absence of catalysts.
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- CAS No.: 1663449-60-2
- Formula: C47H63ClN4O5
- Molecular Weight:799.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 1663449-60-2
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Molecular Weight 799.48
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Formula C47H63ClN4O5
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SMILES
CC1(C)C2=CC=CC=C2[N+](CCCCCC(NCCOCCOCCNC(OC[C@@H]3[C@]4([H])[C@@]3([H])CCC#CCC4)=O)=O)=C1/C=C/C=C5N(C6=CC=CC=C6C\5(C)C)C.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cy3-PEG2-exo-BCN Related Classifications
HY-155327A Related Classifications
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