Cy5 Phosphoramidite
Cy5 Phosphoramidite is a fluorescent labeling reagent. Cy5 Phosphoramidite serves as a fluorescent tag for 3' terminal labeling of single-stranded DNA, enabling fluorescence-based nucleic acid detection, monitoring, quantification, and in vitro study.
For research use only. We do not sell to patients.
- CAS No.: 182873-67-2
- Formula: C60H72ClN4O4P
- Molecular Weight:979.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Cy5 Phosphoramidite-labeled 3'-terminal single-stranded DNA oligonucleotides has sequence-dependent fluorescence intensity[2].
Cy5 Phosphoramidite derivatives can be internally incorporated into DNA sequences via automated synthesis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 182873-67-2
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Molecular Weight 979.66
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Formula C60H72ClN4O4P
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SMILES
N#CCCOP(N(C(C)C)C(C)C)OCCCN1C2=C(C(C)(C)/C1=C\C=C\C=C\C3=[N+](CCCOC(C4=CC=CC=C4)(C5=CC=C(C=C5)OC)C6=CC=CC=C6)C7=C(C3(C)C)C=CC=C7)C=CC=C2.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Meares A, et al. Synthesis of Substituted Cy5 Phosphoramidite Derivatives and Their Incorporation into Oligonucleotides Using Automated DNA Synthesis. ACS Omega. 2022;7(13):11002-11016. Published 2022 Mar 22. [Content Brief]
[2]. Kekić T, et al. Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA. Sci Rep. 2022;12(1):14803. Published 2022 Aug 31. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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