Cy5-UTP 

Cy5-UTP is a fluorescently labeled ribonucleotide triphosphate that can be used as a substrate for terminal deoxynucleotidyl transferase (TdT). Cy5-UTP can be employed to label RNA probes generated in vitro (Ex/Em: 650/665 nm). Cy5-UTP can be applied in FISH, multi-color fluorescence analysis, especially in dual-color expression arrays that combine with Cy5-UTP^[1][2].

Cy5-UTP (100 μM; 1 h) shows minimal bias toward the base composition of immobilized hexamer oligonucleotide probes, making it well suited for quantitative analysis of microarray spots where probes are coupled via a 5' end linkage to the solid phase^[1].
Cy5-UTP (0.1-10% of total UTP pool) enables controllable, high-yield fluorescent co-transcriptional body labeling of FLuc mRNA via T7 RNA polymerase, with a linear relationship between Cy5-UTP input fraction and fluorophores per RNA, and 0.79% Cy5-UTP yielding 5 labels per mRNA^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1178.01

C₄₅H₅₈N₅O₂₂P₃S₂

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Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Guerra CE, et al. Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques. 2006;41(1):53-56.  [Content Brief]

  [2]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017;26(7):1363-1379.  [Content Brief]