Cyanine3B azide
Cyanine3B azide for click chemistry conjugation with terminal alkynes via a copper-catalyzed click reaction or strained cyclooctynes via a copper-free click reaction. Cyanine3B is a yellow-emitting cyanine dye that is an improved version of the Cyanine3 fluorophore with significantly higher fluorescence quantum yield and photostability. Due to the fixed conformation, Cyanine3B has the highest emission quantum yield compared to other dyes of this wavelength. This is a non-sulfonated dye that requires an organic co-solvent (DMF, DMSO, or other) for efficient labeling in water.
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- CAS. Nr.: 1914113-85-1
- Formel: C34H38N6O5S
- Molecular Weight:642.77
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 1914113-85-1
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Molecular Weight 642.77
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Formel C34H38N6O5S
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SMILES
[N-]=[N+]=NCCCNC(CC1=CC(C(C)(C)C2=[N+]3CCC4OC5CCN(C6=CC=C(S(=O)([O-])=O)C=C6C7(C)C)C7=C5C=C24)=C3C=C1)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Reinheit & Dokumentation
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Cyanine3B azide Related Classifications
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