DB772

Cat. No.: HY-114621: FAQs
Data Sheet Handling Instructions

Molarity Calculator

DB772 hydrate is a bovine viral diarrhoea virus (BVDV) inhibitor. DB772 also has anti-prion activity.

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (DB772 hydrate) usually boasts enhanced water solubility and stability.

For research use only. We do not sell to patients.

DB772 Chemical Structure

CAS No. : 1451058-50-6

Size	Stock
5 mg	Get quote	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
50 mg	Get quote	Estimated Time of Arrival: December 31
100 mg	Get quote	Estimated Time of Arrival: December 31

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Other Forms of DB772:

DB772 hydrate In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

DB772 hydrate is a bovine viral diarrhoea virus (BVDV) inhibitor. DB772 also has anti-prion activity^[1].

In Vitro

DB772 hydrate (0-100 μM, 96 hours) can inhibit replication of the viruses BVDV2 and BDV at concentrations greater than 0.2 μM, Bungowannah virus replication at concentrations greater than 0.01 μM, and HoBi virus replication greater than 1.65 μM^[2].
DB772 hydrate has antiprion activity with CC₅₀ (50% cytotoxic concentration) value of 3.8 μM, TC-EC₅₀ (50% tissue culture effective concentration) value of 0. 8 μM and inhibits PrP^Sc accumulation in sheep microglia^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	PrP^Sc-positive and -negative cell lines of human telomerase-immortalized (hTERT) ovine microglia
Concentration:	1 μM
Incubation Time:	4 days
Result:	Showed no significant effect on cell viability with 96.4% cell viability. Significantly reduced PrP^Sc-positive spots by 65.8% and completely inhibited PrP^Sc accumulation in scrapie-infected primary ovine microglia at 4 μM.

Molecular Weight

401.29

Formula

C₂₀H₁₈Cl₂N₄O

CAS No.

1451058-50-6

SMILES

[H]Cl.[H]Cl.C1(C2=CC=C(C3=CC=C(C4=NCCN4)C=C3)O2)=NC5=CC=CC=C5N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (24.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4920 mL	12.4598 mL	24.9196 mL
5 mM	0.4984 mL	2.4920 mL	4.9839 mL
10 mM	0.2492 mL	1.2460 mL	2.4920 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. James B Stanton, et al. Discovery of a novel, monocationic, small-molecule inhibitor of scrapie prion accumulation in cultured sheep microglia and Rov cells. PLoS One. 2012;7(11):e51173. [Content Brief]

[2]. Benjamin W Newcomer, et al. Efficacy of an antiviral compound to inhibit replication of multiple pestivirus species. Antiviral Res. 2012 Nov;96(2):127-9. [Content Brief]

[3]. Kelcey D Dinkel, et al. Antiprion Activity of DB772 and Related Monothiophene- and Furan-Based Analogs in a Persistently Infected Ovine Microglia Culture System. Antimicrob Agents Chemother. 2016 Aug 22;60(9):5467-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4920 mL	12.4598 mL	24.9196 mL	62.2991 mL
	5 mM	0.4984 mL	2.4920 mL	4.9839 mL	12.4598 mL
	10 mM	0.2492 mL	1.2460 mL	2.4920 mL	6.2299 mL
	15 mM	0.1661 mL	0.8307 mL	1.6613 mL	4.1533 mL
	20 mM	0.1246 mL	0.6230 mL	1.2460 mL	3.1150 mL

DB772 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DB7721451058-50-6DB 772DB-772OthersbovineviraldiarrheavirusInhibitorinhibitorinhibit

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