DBCO2-SS2-PEG24 

DBCO2-SS2-PEG24 is a bivalent shielding agent containing dibenzocyclooctyne (DBCO) and PEG24 domains, synthesized for copper-free click reaction-mediated postmodification of azide-bearing siRNA lipo-polyplexes.DBCO2-SS2-PEG24 can be used for the research of cervical carcinoma, hepatocellular carcinoma^[1].

DBCO₂-SS2-PEG₂₄ (0.25-1 equiv (mol/mol) relative to oligomer; 4 h) modified 1216 siRNA polyplexes maintain stable spherical morphology, provide effective charge shielding, exhibit good serum stability, and mediate moderate gene silencing in EGFR-overexpressing KB/eGFPLuc and Huh7/eGFPLuc cells^[1].
DBCO₂-SS2-PEG₂₄ (0.75 equiv (mol/mol) relative to oligomer; 4 h) modified 1216 siRNA polyplexes exhibit lower cellular internalization efficiency in EGFR-overexpressing KB and Huh7 cells compared to GE11-targeted DBCO-modified polyplexes^[1].
DBCO₂-SS2-PEG₂₄ (0.75 equiv (mol/mol) relative to oligomer; 4 h) modified 1216 siRNA polyplexes mediate moderate EG5 gene silencing and antitumoral activity in EGFR-overexpressing KB and Huh7 cells, with lower efficacy than GE11-targeted DBCO-modified polyplexes^[1].
DBCO₂-SS2-PEG₂₄ (0.75 equiv (mol/mol) relative to oligomer; 4 h) modified 1216 siRNA polyplexes induce moderate EG5 silencing-related mitotic arrest and apoptosis in EGFR-overexpressing KB and Huh7 cells, with lower potency than GE11-targeted DBCO-modified polyplexes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2345.88

C₁₁₃H₁₇₃N₉O₃₅S₄

2304536-81-8

O=C(CCC(NCCSSCCCNC(CCC(N[C@H](C(NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(O)=O)=O)CCCCNC(CCC(NCCCSSCCNC(CCC(N1CC2=CC=CC=C2C#CC3=CC=CC=C31)=O)=O)=O)=O)=O)=O)=O)N4CC5=CC=CC=C5C#CC6=CC=CC=C64

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wang Y, et al. Double Click-Functionalized siRNA Polyplexes for Gene Silencing in Epidermal Growth Factor Receptor-Positive Tumor Cells. ACS Biomater Sci Eng. 2020;6(2):1074-1089.