DCP-Bio1 

DCP-Bio1 is a targeting labeling reagent for cysteine sulfenic acid (cysteine sulfenic acid). DCP-Bio1 labels protein cysteine sulfenic acid in intact cells via in situ labeling, and also labels cellular proteins during cell lysis. DCP-Bio1 detects sulfenic acid modifications of FLT3 and FLT3ITD in cancer cells. DCP-Bio1 is applicable to research related to sulfenic acid labeling^[1][2]

DCP-Bio1 (1 mM; variable times) incorporates into sulfenic acid-containing papaya papain, Escherichia coli C84,94S fRMsr, and Salmonella typhimurium C165S AhpC with distinct first-order rates of 1.65 min⁻¹, 0.13 min⁻¹, and 0.003 min⁻¹, respectively^[1].
DCP-Bio1 (0.1-5 mM) incorporation into sulfenic acid-containing proteins in HEK293 cell lysates increases with decreasing protein concentration and increasing DCP-Bio1 concentration, with no difference observed between tumor necrosis factor alpha-treated and untreated cells^[1].
DCP-Bio1 (1 mM; 2 h) labels sulfenic acid residues on wild-type FLT3 in RS4-11 cells and FLT3^ITD in MV4-11 and HEK293 cells, with cysteines 695 and 790 of FLT3^ITD contributing significantly to the labeling signal^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

396.50

C₁₉H₂₈N₂O₅S

882853-94-3

O=C(CC(CC1)=O)C1CCCOC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Klomsiri C, et al.. Use of dimedone-based chemical probes for sulfenic acid detection evaluation of conditions affecting probe incorporation into redox-sensitive proteins. Methods in enzymology. 2010;473:77-94.  [Content Brief]

  [2]. Böhmer A, et al.. Modulation of FLT3 signal transduction through cytoplasmic cysteine residues indicates the potential for redox regulation. Redox biology. 2020 Jan;28:101325.  [Content Brief]