DCP-Bio1
DCP-Bio1 is a targeting labeling reagent for cysteine sulfenic acid (cysteine sulfenic acid). DCP-Bio1 labels protein cysteine sulfenic acid in intact cells via in situ labeling, and also labels cellular proteins during cell lysis. DCP-Bio1 detects sulfenic acid modifications of FLT3 and FLT3ITD in cancer cells. DCP-Bio1 is applicable to research related to sulfenic acid labeling
For research use only. We do not sell to patients.
- CAS No.: 882853-94-3
- Formula: C19H28N2O5S
- Molecular Weight:396.50
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
DCP-Bio1 (1 mM; variable times) incorporates into sulfenic acid-containing papaya papain, Escherichia coli C84,94S fRMsr, and Salmonella typhimurium C165S AhpC with distinct first-order rates of 1.65 min−1, 0.13 min−1, and 0.003 min−1, respectively[1].
DCP-Bio1 (0.1-5 mM) incorporation into sulfenic acid-containing proteins in HEK293 cell lysates increases with decreasing protein concentration and increasing DCP-Bio1 concentration, with no difference observed between tumor necrosis factor alpha-treated and untreated cells[1].
DCP-Bio1 (1 mM; 2 h) labels sulfenic acid residues on wild-type FLT3 in RS4-11 cells and FLT3ITD in MV4-11 and HEK293 cells, with cysteines 695 and 790 of FLT3ITD contributing significantly to the labeling signal[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 882853-94-3
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Molecular Weight 396.50
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Formula C19H28N2O5S
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SMILES
O=C(CC(CC1)=O)C1CCCOC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Klomsiri C, et al.. Use of dimedone-based chemical probes for sulfenic acid detection evaluation of conditions affecting probe incorporation into redox-sensitive proteins. Methods in enzymology. 2010;473:77-94. [Content Brief]
[2]. Böhmer A, et al.. Modulation of FLT3 signal transduction through cytoplasmic cysteine residues indicates the potential for redox regulation. Redox biology. 2020 Jan;28:101325. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DCP-Bio1 Related Classifications
HY-D3473 Related Classifications
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