Fluo-4 potassium
Fluo-4 potassium is a cell-impermeable Ca2+ (calcium ion) indicator (λex=494 nm, λem=516 nm). Fluo-4 potassium can be introduced into cells using methods such as patch clamping and microinjection.
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- CAS. Nr.: 1199351-32-0
- Formel: C36H25F2K5N2O13
- Molecular Weight:927.08
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 1199351-32-0
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Molecular Weight 927.08
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Formel C36H25F2K5N2O13
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SMILES
O=C1C=C2OC3=C(C(C4=CC(OCCOC5=CC(C)=CC=C5N(CC(O[K])=O)CC(O[K])=O)=C(N(CC(O[K])=O)CC(O[K])=O)C=C4)=C2C=C1F)C=C(F)C(O[K])=C3
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)