huDMB5F3

huDMB5F3 (0.4-7 nM; 4-min association, 30-min dissociation) binds to recombinant MUC1-X protein with extremely high affinity (K_d = 5.89 pM), whereas the monovalent Fab fragment exhibits 100-fold lower affinity due to reduced avidity (K_d = 4.84 nM)^[1].
huDMB5F3 (62 ng/mL-4 μg/mL; 1-h primary antibody incubation, 45-min secondary antibody incubation) binds specifically to human breast cancer ZR75 cells expressing MUC1, and a binding signal is detectable at concentrations as low as 62 ng/mL (0.4 nM)^[1].
huDMB5F3 (incubated at 4°C or 37°C) is efficiently internalized into MUC1-expressing cells in a temperature-dependent manner, localizing to the cell membrane at 4°C and entering the intracellular space at 37°C^[1].
huDMB5F3 (0.001-1000 pM; incubated with cells following 5-h seeding) potently and selectively kills MUC1-expressing DA3-TM, T47D, Colo357 and ZR75 cancer cells, with an IC₅₀ value of approximately 16 pM in Colo357 cells and approximately 3 pM in ZR75 cells; soluble MUC1-Xex abrogates its cytotoxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.