4-Azidophenol
Based on 1 Customer Validation
4-Azidophenol (p-Azidophenol) is a click chemistry reagent containing an azide group that can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. 4-Azidophenol can also react with molecules containing DBCO or BCN groups through strain-promoted azide-alkyne cycloaddition (SPAAC).
For research use only. We do not sell to patients.
- Purity : 99.83%
- CAS No.: 24541-43-3
- Formula: C6H5N3O
- Molecular Weight:135.12
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 24541-43-3
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Appearance Oil
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Molecular Weight 135.12
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Formula C6H5N3O
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Color Brown to black
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SMILES
C1=C(C=CC(=C1)O)N=[N+]=[N-]
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Synonyms
p-Azidophenol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro:
DMSO : 5 mg/mL (37.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (643 KB)
- English - EN (643 KB)
- Français - FR (643 KB)
- Deutsch - DE (643 KB)
- Norwegian - NO (643 KB)
- Español - ES (643 KB)
- Swedish - SV (643 KB)
- Italian - IT (643 KB)
- Korean - KR (643 KB)
- Portuguese - PT (643 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.4008 mL | 37.0041 mL | 74.0083 mL | 185.0207 mL |
| 5 mM | 1.4802 mL | 7.4008 mL | 14.8017 mL | 37.0041 mL | |
| 10 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | 18.5021 mL | |
| 15 mM | 0.4934 mL | 2.4669 mL | 4.9339 mL | 12.3347 mL | |
| 20 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL | 9.2510 mL | |
| 25 mM | 0.2960 mL | 1.4802 mL | 2.9603 mL | 7.4008 mL | |
| 30 mM | 0.2467 mL | 1.2335 mL | 2.4669 mL | 6.1674 mL |
4-Azidophenol Related Classifications
HY-W178441 Related Classifications
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