DFBTA
Based on 1 Customer Validation
DFBTA is an orally active and potent ANO1 (Calcium-activated chloride channel anoctamin-1) inhibitor, with an IC50 of 24 nM. DFBTA shows analgesic efficacy for inflammatory pain.
For research use only. We do not sell to patients.
- Purity: 99.25%
- CAS No.: 2966044-07-3
- Formula: C18H10ClF2NO3S
- Molecular Weight:393.79
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
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Biological Activity
IC50: 0.024 ± 0.012 μM (ANO1), 8.7 ± 1.0 μM (ANO2)[1]
DFBTA shows very weak cytotoxicity and cardiotoxicity (HEK293 proliferation IC50 > 30 μM, hERG IC50 > 30 μM)[1]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
DFBTA (C57BL/6 mice, 1000 mg/kg, Orally, once) shows excellent pharmacokinetics properties with oral bioavailability > 75% and little brain penetration (<1.5% brain/plasma)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2966044-07-3
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Appearance Solid
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Molecular Weight 393.79
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Formula C18H10ClF2NO3S
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Color White to off-white
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SMILES
O=C(C1=C(SC=C1C2=CC=C(C=C2)Cl)NC(C3=C(C=CC(F)=C3)F)=O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (253.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5394 mL | 12.6971 mL | 25.3942 mL | 63.4856 mL |
| 5 mM | 0.5079 mL | 2.5394 mL | 5.0788 mL | 12.6971 mL | |
| 10 mM | 0.2539 mL | 1.2697 mL | 2.5394 mL | 6.3486 mL | |
| 15 mM | 0.1693 mL | 0.8465 mL | 1.6929 mL | 4.2324 mL | |
| 20 mM | 0.1270 mL | 0.6349 mL | 1.2697 mL | 3.1743 mL | |
| 25 mM | 0.1016 mL | 0.5079 mL | 1.0158 mL | 2.5394 mL | |
| 30 mM | 0.0846 mL | 0.4232 mL | 0.8465 mL | 2.1162 mL | |
| 40 mM | 0.0635 mL | 0.3174 mL | 0.6349 mL | 1.5871 mL | |
| 50 mM | 0.0508 mL | 0.2539 mL | 0.5079 mL | 1.2697 mL | |
| 60 mM | 0.0423 mL | 0.2116 mL | 0.4232 mL | 1.0581 mL | |
| 80 mM | 0.0317 mL | 0.1587 mL | 0.3174 mL | 0.7936 mL | |
| 100 mM | 0.0254 mL | 0.1270 mL | 0.2539 mL | 0.6349 mL |
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