Dipotassium glycyrrhizinate
Based on 59 publication(s) in Google Scholar
Dipotassium glycyrrhizinate is a HMGB1 inhibitor, inhibits atopic dermatitis-related gene expression with anti-anti-inflammatory activity.
For research use only. We do not sell to patients.
- Purity: 99.68%
- CAS No.: 68797-35-3
- Formula: C42H60K2O16
- Molecular Weight:899.11
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications Citing Use of MedChemExpress (MCE) Dipotassium glycyrrhizinate
More- Nat Nanotechnol. 2025 May 19. [Abstract]
- Adv Sci (Weinh). 2024 Aug 5:e2404408. [Abstract]
- Chem Eng J. 2025 May 1.
- J Exp Clin Cancer Res. 2024 Apr 4;43(1):105. [Abstract]
- Mater Today Bio. 2025 Dec 8:36:102657. [Abstract]
- Mater Today Bio. 2025 Apr 17:32:101778. [Abstract]
- Adv Healthc Mater. 2024 Jan;13(2):e2301808. [Abstract]
- Cell Commun Signal. 2023 May 1;21(1):86. [Abstract]
- Phytomedicine. 2025 Sep:145:157081. [Abstract]
- Phytomedicine. 2025 Jul:142:156713. [Abstract]
- Phytomedicine. 2024 Dec:135:156059. [Abstract]
- Food Res Int. 2025 Dec;222(Pt 1):117620. [Abstract]
- Redox Rep. 2025 Dec;30(1):2585221. [Abstract]
- Cell Death Discov. 2024 Jan 17;10(1):34. [Abstract]
- EMBO Rep. 2025 Nov 17. [Abstract]
- Cell Prolif. 2024 Nov 20:e13774. [Abstract]
- Cell Prolif. 2020 Jun;53(6):e12829. [Abstract]
- Drug Des Devel Ther. 2025 Apr 2:19:2503-2517. [Abstract]
- CNS Neurosci Ther. 2025 May;31(5):e70406. [Abstract]
- Pharmaceuticals (Basel). 2022 Jan 31;15(2):179. [Abstract]
- Pharm Biol. 2020 Dec;58(1):898-904. [Abstract]
- Eur J Pharmacol. 2025 Oct 5:1004:177971. [Abstract]
- Int Immunopharmacol. 2025 Mar 26:150:114294. [Abstract]
- Int Immunopharmacol. 2025 Feb 3:149:114192. [Abstract]
- Neuropharmacology. 2025 Nov 15:279:110617. [Abstract]
- Hepatol Commun. 2023 Dec 22;8(1):e0350. [Abstract]
- Rheumatology (Oxford). 2025 Aug 13:keaf437. [Abstract]
- Mol Neurobiol. 2025 May;62(5):6070-6087. [Abstract]
- Front Cell Dev Biol. 2020 Aug 11;8:713. [Abstract]
- J Cell Mol Med. 2025 Nov;29(21):e70944. [Abstract]
- Mediators Inflamm. 2025 Jul 11:2025:2395557. [Abstract]
- J Inflamm Res. 2025 May 25:18:6735-6748. [Abstract]
- J Funct Foods. 2021, 104584.
- Sci Rep. 2025 Nov 12;15(1):39708. [Abstract]
- Fish Shellfish Immunol. 2024 Jan:144:109261. [Abstract]
- ACS Chem Neurosci. 2020 Mar 18;11(6):979-993. [Abstract]
- Aging (Albany NY). 2019 Jul 7;11(13):4323-4337. [Abstract]
- Cell Signal. 2024 Mar:115:111036. [Abstract]
- Eur J Med Res. 2025 Oct 9;30(1):943. [Abstract]
- Cardiovasc Drugs Ther. 2026 Jan 24. [Abstract]
- 3 Biotech. 2020 Aug;10(8):369. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Toxicol In Vitro. 2025 Oct:108:106104. [Abstract]
- BMC Immunol. 2021 May 27;22(1):34. [Abstract]
- Adv Biol (Weinh). 2025 Jun 4:e00761. [Abstract]
- Behav Brain Res. 2025 May 24:115662. [Abstract]
- Biochem Biophys Res Commun. 2021 Feb 12:540:22-28. [Abstract]
- World Neurosurg. 2022 Feb:158:e856-e864. [Abstract]
- Planta Med. 2021 Jun;87(7):538-549. [Abstract]
- Rev Bras Farmacogn. 2023 Jun 28.
- SSRN. 2025 Nov 20.
- Res Sq. 2025 Oct 28.
- SSRN. 2025 Jul 8.
- bioRxiv. 2025 Feb 19.
- SSRN. 2025 Jan 15.
- Research Square Preprint. 2023 Apr 27.
- Research Square Print. August 24th, 2022.
- Research Square Preprint. 2022 May.
- Evid Based Complement Alternat Med. 2020 May 31;2020:8653783. [Abstract]
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WB
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In Vivo Efficacy Study
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RT-PCR
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Histological Imaging/Staining
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Biological Activity
Dipotassium glycyrrhizinate (0-400 μM, 4 days) inhibits mRNA level of atopic dermatitis related genes (NELL2, CA2, AQP3, and HAS3 gene level) in IL‑4‑ and IL‑13‑induced keratinocytes[1].
Dipotassium glycyrrhizinate (0-400 μM, 4 days) partially repaired the atopic dermatitis-like phenotype (the spongiosis like intercellular spaces) in the IL-4/IL-13-induced AD-like skin equivalent model[1].
Dipotassium glycyrrhizinate (0-100 μM, 24 h) inhibits Sunitinib-induced autophagy, cardiomyocyte death in CCC-HEH-2 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Dipotassium glycyrrhizinate (50 mg/kg, i.p., once daily for 4 weeks) inhibits thyroiditis in the Iodine-induced murine model of autoimmune thyroiditis[3].
Dipotassium glycyrrhizinate (50-200 mg/kg, i.p.,) inhibits Bleomycin (HY-108345)-induced pulmonary fibrosis IN rats[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Iodine-induced murine model of autoimmune thyroiditis[3]
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Dosage:50 mg/kg
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Administration:i.p., once daily for 4 weeks
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Result:Reduced serum levels of TgAb, HMGB1, TNF-α, IL-6, IL-1β.
Decreased The prevalence of thyroiditis and the infiltration of lymphocytes, and attenuated the severity of thyroiditis.
Chemical Information
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CAS No. 68797-35-3
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Appearance Solid
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Molecular Weight 899.11
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Formula C42H60K2O16
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Color White to off-white
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SMILES
C[C@]12[C@@](C(C=C3[C@]2(CC[C@]4(C)[C@@]3([H])C[C@](C(O[K])=O)(C)CC4)C)=O)([H])[C@@]5([C@@](C(C)([C@@H](O[C@]6([H])[C@@H]([C@H]([C@H](O)[C@@H](C(O)=O)O6)O)O[C@]7([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]7O)C(O[K])=O)CC5)C)([H])CC1)C
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Synonyms
Glycyrrhizic acid dipotassium; Dipotassium glycyrrhizate
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (59)
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Journal Impact Factor
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Most Recent
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Nat Nanotechnol
Tumour-derived microparticles obtained through microwave irradiation induce immunogenic cell death in lung adenocarcinoma. [Abstract]2025 May 19. PMID: 40389640 -
Adv Sci (Weinh)
Generation of Anti-Mastitis Gene-Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2-TnpB System. [Abstract]2024 Aug 5:e2404408. PMID: 39099401 -
Dipotassium glycyrrhizinate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 May 1.
The protein expression of P21, P16, Tom20, HMGB1, cGAS, and STING were assessed via western blotting with β-tubulin as a loading control. Data were expressed as mean ± SEM (n = 3). The results showed that Glycyrrhizic acid (10 μM; 24 h) significantly enhanced the anti-senescent effect of exosomes and inhibited the HMGB1/cGAS-STING axis.
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J Exp Clin Cancer Res
GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression. [Abstract]2024 Apr 4;43(1):105. PMID: 38576043
Dipotassium glycyrrhizinate purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2024 Apr 4;43(1):105. [Abstract]
Glycyrrhizin (1 nM/kg; i.c.v.; administered every 3 days starting from day 14 after modeling). A, In a subcutaneous xenograft tumor model, the tumor volumes were measured and monitored every 7 days from each group of mice. (n = 4/group). B, The pictures of corresponding subcutaneous xenograft tumors dissected from each group mice at 42 days post-injection. C Tumor weights from each group at 42 days post-injection.
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Mater Today Bio
Glycyrrhizic acid-loaded biomimetic hybrid liposomes targeting inflammatory cascades and PD-1/PD-L1 pathway to reverse neuroinflammation-driven cognitive decline. [Abstract]2025 Dec 8:36:102657. PMID: 41476753 -
Mater Today Bio
Membrane biomimetic nanoenzyme-incorporated hybrid glycyrrhizic acid hydrogel for precise mitochondrial ROS scavenging for osteoarthritis treatment. [Abstract]2025 Apr 17:32:101778. PMID: 40290887 -
Adv Healthc Mater
Fn-HMGB1 Adsorption Behavior Initiates Early Immune Recognition and Subsequent Osteoinduction of Biomaterials. [Abstract]2024 Jan;13(2):e2301808. PMID: 37602504
Dipotassium glycyrrhizinate purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2024 Jan;13(2):e2301808. [Abstract]
Osteoinduction was hindered in Glycyrrhizic acid (GA, 10 mM; 30min) group.
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Cell Commun Signal
Neutrophil extracellular traps induced by the hypoxic microenvironment in gastric cancer augment tumour growth. [Abstract]2023 May 1;21(1):86. PMID: 37127629 -
Phytomedicine
Amoebicidal action of isoliquiritigenin and glabridin from Glycyrrhiza species: Mechanisms and effects against Acanthamoeba castellanii. [Abstract]2025 Sep:145:157081. PMID: 40701128 -
Phytomedicine
Bioactive equivalent combinatorial components of Xiao-Xu-Ming decoction inhibit the calmodulin-mediated MLCK/MLC axis to attenuate coronary artery spasm. [Abstract]2025 Jul:142:156713. PMID: 40349422 -
Phytomedicine
Glycyrrhizin alleviates the toxicity of hydroxychloroquine in treating oral lichen planus by occupying heat shock protein 90 alpha. [Abstract]2024 Dec:135:156059. PMID: 39550918
Dipotassium glycyrrhizinate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156059. [Abstract]
qRT-PCR analysis of HSP90AB1 and HSP90AA1 in NOK cells after stimulation with LPS and HCQ (10 µM) or Glycyrrhiz (GL, 100 μg/mL) in for 24 h.
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Food Res Int
Glycyrrhiza uralensis Fisch: A novel source of analgesic activity through NaV1.8 sodium channel modulation. [Abstract]2025 Dec;222(Pt 1):117620. PMID: 41267240 -
Redox Rep
Monoammonium glycyrrhizinate ameliorates mitochondrial dysfunction-mediated oxidative stress and neuroinflammation via the NRF2/NQO1 axis after spinal cord injury. [Abstract]2025 Dec;30(1):2585221. PMID: 41240391 -
Cell Death Discov
2024 Jan 17;10(1):34. PMID: 38233385 -
EMBO Rep
Aspergillus fumigatus promotes tumor angiogenesis via SLC7A11 on myeloid-derived suppressor cells. [Abstract]2025 Nov 17. PMID: 41249582 -
Cell Prolif
2024 Nov 20:e13774. PMID: 39566909 -
Cell Prolif
TNF-α/HMGB1 inflammation signalling pathway regulates pyroptosis during liver failure and acute kidney injury. [Abstract]2020 Jun;53(6):e12829. PMID: 32419317
Dipotassium glycyrrhizinate purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2020 Jun;53(6):e12829. [Abstract]
Protein levels of pro-caspase-1 + p10 + p12, GADMD, IL-1β, IL-18 and NLRP3 are detected by Western blotting. Glycyrrhizin inhibits pyroptosis in TNF-α-induced M1 macrophages.
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Drug Des Devel Ther
The Jieduan-Niwan Formula Reduces Inflammatory Responses in Acute-on-Chronic Liver Failure Rats by Inhibiting HMGB1-Induced Hepatocyte Pyroptosis. [Abstract]2025 Apr 2:19:2503-2517. PMID: 40190810 -
CNS Neurosci Ther
11,12-Diacetyl-Carnosol Ameliorates Depression-Like Behaviors and Memory Dysfunction in CUMS Mouse Model via Inhibiting HMGB1-Mediated Neuroinflammation. [Abstract]2025 May;31(5):e70406. PMID: 40406924 -
Pharmaceuticals (Basel)
Bruceine D Identified as a Drug Candidate against Breast Cancer by a Novel Drug Selection Pipeline and Cell Viability Assay. [Abstract]2022 Jan 31;15(2):179. PMID: 35215292 -
Pharm Biol
Sesamin attenuates intestinal injury in sepsis via the HMGB1/TLR4/IL-33 signalling pathway. [Abstract]2020 Dec;58(1):898-904. PMID: 32893702 -
Eur J Pharmacol
HBHP ameliorates CCL4-induced liver fibrosis in rats by inhibiting HMGB1-mediated inflammatory response through binding to the HMGB1-A box. [Abstract]2025 Oct 5:1004:177971. PMID: 40683441 -
Int Immunopharmacol
Glycyrrhizin alleviates brain injury in necrotizing enterocolitis model mice by suppressing HMGB1/TLR4 pathway. [Abstract]2025 Mar 26:150:114294. PMID: 39970710 -
Int Immunopharmacol
Inhibiting HMGB1/AGER/NF-κB pathway prevents pro-inflammatory microglia polarization and protect photoreceptors in retinitis pigmentosa. [Abstract]2025 Feb 3:149:114192. PMID: 39904032 -
Neuropharmacology
Zinc induces neuronal autophagy via HMGB1 nuclear translocation in acute cerebral ischemia. [Abstract]2025 Nov 15:279:110617. PMID: 40752584 -
Hepatol Commun
Aging aggravates liver fibrosis through downregulated hepatocyte SIRT1-induced liver sinusoidal endothelial cell dysfunction. [Abstract]2023 Dec 22;8(1):e0350. PMID: 38126919 -
Rheumatology (Oxford)
Autophagy inhibitors block pathogenic NET release in immune-mediated inflammatory disease without impairing host defence. [Abstract]2025 Aug 13:keaf437. PMID: 40802538 -
Mol Neurobiol
Glycyrrhizin Attenuates White Matter Injury by Inhibiting Neuroinflammation through the HMGB1/TLR4 Pathway. [Abstract]2025 May;62(5):6070-6087. PMID: 39707121 -
Front Cell Dev Biol
Nucleotide-Binding Oligomerization Domain-Like Receptor 3 Deficiency Attenuated Isoproterenol-Induced Cardiac Fibrosis via Reactive Oxygen Species/High Mobility Group Box 1 Protein Axis. [Abstract]2020 Aug 11;8:713. PMID: 32850832 -
J Cell Mol Med
Jinbei Decoction Attenuates LPS-Induced Acute Lung Injury via Suppression of TRAF6-Dependent Inflammatory Response in Macrophage. [Abstract]2025 Nov;29(21):e70944. PMID: 41230791 -
Mediators Inflamm
HMGB1 Exacerbates Intestinal Barrier Damage by Inducing Ferroptosis Through the TLR4/NF-κB/GPX4 Pathway in Ulcerative Colitis. [Abstract]2025 Jul 11:2025:2395557. PMID: 40689397 -
J Inflamm Res
HMGB1 Inhibition Alleviates Chronic Nonbacterial Prostatitis by Suppressing M1 Polarization of Macrophages. [Abstract]2025 May 25:18:6735-6748. PMID: 40453963 -
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Sci Rep
Xiaoyao San against depression via glia-mediated neuroinflammation using network pharmacology and experimental validation. [Abstract]2025 Nov 12;15(1):39708. PMID: 41225027 -
Fish Shellfish Immunol
Grass carp Il-2 promotes neutrophil extracellular traps formation via inducing ROS production and autophagy in vitro. [Abstract]2024 Jan:144:109261. PMID: 38040137 -
ACS Chem Neurosci
Alarmin HMGB1 Plays a Detrimental Role in Hippocampal Dysfunction Caused by Hypoxia-Ischemia Insult in Neonatal Mice: Evidence from the Application of the HMGB1 Inhibitor Glycyrrhizin. [Abstract]2020 Mar 18;11(6):979-993. PMID: 32073822 -
Aging (Albany NY)
2019 Jul 7;11(13):4323-4337. PMID: 31284269 -
Cell Signal
Glycyrrhizic acid alleviated MI/R-induced injuries by inhibiting Hippo/YAP signaling pathways. [Abstract]2024 Mar:115:111036. PMID: 38185229 -
Eur J Med Res
HMGB1 regulates autophagy via TLR4/NF-κB signaling pathway in myocardial injury induced by coronary microembolization. [Abstract]2025 Oct 9;30(1):943. PMID: 41068984 -
Cardiovasc Drugs Ther
High Mobility Group Protein B1 Mediates the Role of the Neutrophil Extracellular Traps in the Progression of Acute Myocardial Infarction. [Abstract]2026 Jan 24. PMID: 41579229 -
3 Biotech
Lycorine attenuates lipopolysaccharide-induced acute lung injury through the HMGB1/TLRs/NF-κB pathway. [Abstract]2020 Aug;10(8):369. PMID: 32818131 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Toxicol In Vitro
The protective mechanisms of glycyrrhizic acid for deoxynivalenol-induced toxicity in rabbit corneal stroma. [Abstract]2025 Oct:108:106104. PMID: 40617304 -
BMC Immunol
Glycyrrhizin improves the pathogenesis of psoriasis partially through IL-17A and the SIRT1-STAT3 axis. [Abstract]2021 May 27;22(1):34. PMID: 34044769 -
Adv Biol (Weinh)
HMGB1 Derived from the Pyroptotic Microenvironment Promotes Macrophage Extracellular Traps in Hirschsprung-Associated Enterocolitis. [Abstract]2025 Jun 4:e00761. PMID: 40464207 -
Behav Brain Res
HMGB1/RAGE signaling mediates the activation of microglia and participates in depressive-like behaviors and cognitive deficits in rats after ischemia-reperfusion. [Abstract]2025 May 24:115662. PMID: 40419119 -
Biochem Biophys Res Commun
Uric acid inhibits HMGB1-TLR4-NF-κB signaling to alleviate oxygen-glucose deprivation/reoxygenation injury of microglia. [Abstract]2021 Feb 12:540:22-28. PMID: 33429196 -
World Neurosurg
Glycyrrhizic Acid Attenuates the Inflammatory Response After Spinal Cord Injury by Inhibiting High Mobility Group Box-1 Protein Through the p38/Jun N-Terminal Kinase Signaling Pathway. [Abstract]2022 Feb:158:e856-e864. PMID: 34838764 -
Planta Med
Compound Cocktail Inhibits Influenza Viral Pneumonia via Phospholipase Cγ1 Phosphorylation-Related Necroptosis and Partial Autophagy in Natural Killer Cells. [Abstract]2021 Jun;87(7):538-549. PMID: 33545719 -
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Evid Based Complement Alternat Med
Glycyrrhizin Ameliorates Radiation Enteritis in Mice Accompanied by the Regulation of the HMGB1/TLR4 Pathway. [Abstract]2020 May 31;2020:8653783. PMID: 32595744
Solvent & Solubility
H2O : 50 mg/mL (55.61 mM; Need ultrasonic)
DMSO : 17.5 mg/mL (19.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 16.67 mg/mL (18.54 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Lee SH, et al. Ameliorating effect of dipotassium glycyrrhizinate on an IL-4- and IL-13-induced atopic dermatitis-like skin-equivalent model. Arch Dermatol Res. 2019 Mar;311(2):131-140. [Content Brief]
[2]. Wang LY, et al. Glycyrrhizic acid increases glucagon like peptide-1 secretion via TGR5 activation in type 1-like diabetic rats. Biomed Pharmacother. 2017 Nov;95:599-604. [Content Brief]
[3]. Li C, et al. Glycyrrhizin, a Direct HMGB1 Antagonist, Ameliorates Inflammatory Infiltration in a Model of Autoimmune Thyroiditis via Inhibition of TLR2-HMGB1 Signaling. Thyroid. 2017 May;27(5):722-731. [Content Brief]
[4]. Xu Z, et al. Autophagic degradation of CCN2 (cellular communication network factor 2) causes cardiotoxicity of sunitinib. Autophagy. 2022 May;18(5):1152-1173. [Content Brief]
[5]. Gao L, et al. Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats. Front Pharmacol. 2015 Oct 1;6:215. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.1122 mL | 5.5611 mL | 11.1221 mL | 27.8053 mL |
| 5 mM | 0.2224 mL | 1.1122 mL | 2.2244 mL | 5.5611 mL | |
| 10 mM | 0.1112 mL | 0.5561 mL | 1.1122 mL | 2.7805 mL | |
| 15 mM | 0.0741 mL | 0.3707 mL | 0.7415 mL | 1.8537 mL | |
| H2O | 20 mM | 0.0556 mL | 0.2781 mL | 0.5561 mL | 1.3903 mL |
| 25 mM | 0.0445 mL | 0.2224 mL | 0.4449 mL | 1.1122 mL | |
| 30 mM | 0.0371 mL | 0.1854 mL | 0.3707 mL | 0.9268 mL | |
| 40 mM | 0.0278 mL | 0.1390 mL | 0.2781 mL | 0.6951 mL | |
| 50 mM | 0.0222 mL | 0.1112 mL | 0.2224 mL | 0.5561 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.