2. Others
  3. Biochemical Assay Reagents
  4. Direct Black 38

Direct Black 38  (Synonyms: Chlorazol Black E; Ferristatin II disodium; C.I. 30235)

Cat. No.: HY-D0256
COA
SDS
Handling Instructions Technical Support

Direct Black 38 (Chlorazol Black E) is a benzidine-based azo dye, a substrate of Enterococcus gallinarum, and is mainly used for textile dyeing. Direct Black 38 is biodegradable after being reduced by microbial enzymes (such as Enterococcus gallinarum azoreductase), breaking the azo bonds and releasing benzidine and 4-aminobiphenyl. Direct Black 38 can be used in environmental toxicology, wastewater treatment and carcinogenicity studies, but its metabolite benzidine is listed as a human carcinogen.

For research use only. We do not sell to patients.

Direct Black 38

Direct Black 38 Chemical Structure

CAS No. : 1937-37-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 g In-stock
5 g In-stock
10 g In-stock
25 g In-stock
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Direct Black 38:

Direct Black 38 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Direct Black 38 (Chlorazol Black E) is a benzidine-based azo dye, a substrate of Enterococcus gallinarum, and is mainly used for textile dyeing. Direct Black 38 is biodegradable after being reduced by microbial enzymes (such as Enterococcus gallinarum azoreductase), breaking the azo bonds and releasing benzidine and 4-aminobiphenyl. Direct Black 38 can be used in environmental toxicology, wastewater treatment and carcinogenicity studies, but its metabolite benzidine is listed as a human carcinogen[1].

In Vitro

In the basal medium experiment, Direct Black 38 (25-100 mg/L; 24 h) is decolorized by 53-63% by Enterococcus gallinarum, releasing benzidine and 4-aminobiphenyl[1].
In the Luria broth experiment, Direct Black 38 (20-250 mg/L; 48 h) achieves a decolorization rate of 71-85%, with higher biomass efficiency, in line with Monod kinetics (Vmax=12.8 mg·L-1·h-1), Ks=490.6 mg·L-1)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Direct Black 38 Related Antibodies
Molecular Weight

781.73

Formula

C34H25N9Na2O7S2
CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

O=S(C1=C(/N=N/C2=CC=C(C3=CC=C(/N=N/C4=CC=C(N)C=C4N)C=C3)C=C2)C(N)=C5C(O)=C(/N=N/C6=CC=CC=C6)C(S(=O)(O[Na])=O)=CC5=C1)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (31.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (6.40 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2792 mL 6.3961 mL 12.7921 mL
5 mM 0.2558 mL 1.2792 mL 2.5584 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

SDS (419 KB)

COA (247 KB) IR (176 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2792 mL 6.3961 mL 12.7921 mL 31.9804 mL
5 mM 0.2558 mL 1.2792 mL 2.5584 mL 6.3961 mL
DMSO 10 mM 0.1279 mL 0.6396 mL 1.2792 mL 3.1980 mL
15 mM 0.0853 mL 0.4264 mL 0.8528 mL 2.1320 mL
20 mM 0.0640 mL 0.3198 mL 0.6396 mL 1.5990 mL
25 mM 0.0512 mL 0.2558 mL 0.5117 mL 1.2792 mL
30 mM 0.0426 mL 0.2132 mL 0.4264 mL 1.0660 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Direct Black 38 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Direct Black 381937-37-7Chlorazol Black E Ferristatin II disodium C.I. 30235Direct Black38Direct Black-38Biochemical Assay ReagentsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Direct Black 38
Cat. No.:
HY-D0256
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (419 KB) Français - FR (419 KB) Deutsch - DE (419 KB) Norwegian - NO (419 KB) Español - ES (419 KB) Swedish - SV (419 KB) Italian - IT (419 KB) Korean - KR (419 KB) Portuguese - PT (419 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.