1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis
  3. Direct Black 38

Direct Black 38  (Synonyms: Chlorazol Black E; Ferristatin II disodium; C.I. 30235)

Cat. No.: HY-D0256
COA Handling Instructions

Direct Black 38 (Chlorazol Black E) is an azo dye. Direct Black 38 induces unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats in vivo.

For research use only. We do not sell to patients.

Direct Black 38 Chemical Structure

Direct Black 38 Chemical Structure

CAS No. : 1937-37-7

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Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Direct Black 38:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

Direct Black 38 (Chlorazol Black E) is an azo dye. Direct Black 38 induces unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats in vivo[1].

Molecular Weight

781.73

Appearance

Solid

Formula

C34H25N9Na2O7S2

CAS No.
SMILES

O=S(C1=C(/N=N/C2=CC=C(C3=CC=C(/N=N/C4=CC=C(N)C=C4N)C=C3)C=C2)C(N)=C5C(O)=C(/N=N/C6=CC=CC=C6)C(S(=O)(O[Na])=O)=CC5=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (31.98 mM; Need ultrasonic)

H2O : 5 mg/mL (6.40 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2792 mL 6.3961 mL 12.7921 mL
5 mM 0.2558 mL 1.2792 mL 2.5584 mL
10 mM 0.1279 mL 0.6396 mL 1.2792 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 3.85 mg/mL (4.92 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Direct Black 38
Cat. No.:
HY-D0256
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