TTX-P
TTX-P is a fluorescent probe. TTX-P responds in situ to the overexpressed alkaline phosphatase (ALP) in liver, imaging of diabetic liver injury in the near-infrared second-window (NIR-II) region.
For research use only. We do not sell to patients.
- Formula: C46H35N4O5PS
- Molecular Weight:786.83
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
TTX-P (10 μM) is responsive to alkaline phosphatase (ALP) and correspondingly emits NIR-II fluorescent signals which are suitable for imaging (at 920 nm)[1].
TTX-P (10 μM; HepG2, 4T1, and LO2 cells) can detect and monitor the activity of ALP in cancer cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/C nude mice with 4T1 cells xenografts (female, 4-5 weeks old)[1]
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Dosage:5 mg/kg
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Administration:Intratumoral injection
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Result:Enhanced NIR-II fluorescence signals in a time-dependent manner.
Chemical Information
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Molecular Weight 786.83
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Formula C46H35N4O5PS
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SMILES
CC1=CC=C(C=C1)N(C2=CC=C(C=C2)C3=N/C(S/C3=C\C4=C5OC6=C(C=C5CCC4)C=CC(C7=CC=C(C=C7)OP(O)(O)=O)=C6)=C(C#N)/C#N)C8=CC=C(C=C8)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TTX-P Related Classifications
HY-149835 Related Classifications
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