DNA2 inhibitor C5

Name: DNA2 inhibitor C5
Brand: MedChemExpress
SKU: HY-128729
Rating: 5.0 (10 reviews)

Cat. No.: HY-128729 Purity: 98.51%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DNA2 inhibitor C5 is a potent, competitive, and specific DNA2 nuclease inhibitor with an IC₅₀ of 20 μM. DNA2 inhibitor C5 inhibits the nuclease, DNA-dependent ATPase, helicase, and DNA-binding activities of DNA2. DNA2 inhibitor C5 can be used in breast cancer and colorectal cancer research.

For research use only. We do not sell to patients.

CAS No. : 35973-25-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of DNA2 inhibitor C5:

DNA2 inhibitor C5 (Standard) In-stock

10 Publications Citing Use of MCE DNA2 inhibitor C5

Cell Imaging/Staining

Bio/Physico-chemical Assay

: DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 7. [Abstract]; DNA2 inhibitor C5 (20 μM; 24 h) significantly reduced ssDNA gap formation in EP300Mut cells.

: DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 25;16(1):7901. [Abstract]; DNA2 inhibitor C5 (100 μM; 30 min). Representative immunofluorescence images and quantification of prometaphase U2OS cells with MiDAS.

: DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Oct;634(8033):492-500. [Abstract]; DNA2 inhibitor C5 (DNA2i) (20 μM; 6 h) reduced DNA end resection in CtIP wild-type cells.

: DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Oct;634(8033):492-500. [Abstract]; Quantitation of the cell cycle distribution of the U2OS-derived cell lines treated with the DNA2 inhibitor C5 (DNA2i) (20 μM; 6 h).

: DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2021 Jul 15;81(14):3007-3017.e5. [Abstract]; The fork degradation caused by partial depletion of RADX siRNA was prevented by treatment with DNA2 inhibitor C5 (20 μM).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Helicase

Cellular Effect

Cell Line	Type	Value	Description	References
A549	CC₅₀	> 100 μM Compound: 2, NSC-15765	Cytotoxicity against human A549 cells after 48 hrs Cytotoxicity against human A549 cells after 48 hrs	[PMID: 20108931]
A549	EC₅₀	> 100 μM Compound: 2, NSC-15765	Antiviral activity against Dengue virus type 2 infected in human A549 cells assessed as decrease in viral E protein production by cell-based flavivirus immunodetection assay Antiviral activity against Dengue virus type 2 infected in human A549 cells assessed as decrease in viral E protein production by cell-based flavivirus immunodetection assay	[PMID: 20108931]

In Vitro

DNA2 inhibitor C5 (0-250 μM) inhibits the nuclease activity of DNA2^[1].
DNA2 inhibitor C5 (0-10 μM) has a strong synergistic effect in inhibiting MCF7 cell survival with MK4827, with IC₅₀ values of 0.8 μM and 1.9 μM respectively and a combination index of 0.13^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

DNA2 inhibitor C5 shows synthetic lethality with deficiencies in FEN1, EXO1, or MRE11 genes in the mouse model with HCT116^fox/- cells carrying a conditional genetic knockout of DNA2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

234.17

Formula

C₁₀H₆N₂O₅

CAS No.

35973-25-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=C(O)C2=CC=CC([N+]([O-])=O)=C2N=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (142.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2704 mL	21.3520 mL	42.7040 mL
5 mM	0.8541 mL	4.2704 mL	8.5408 mL
10 mM	0.4270 mL	2.1352 mL	4.2704 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.51%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (252 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Binghui Shen, et al. Dna2 inhibitors for cancer treatment. US20170226063A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2704 mL	21.3520 mL	42.7040 mL	106.7600 mL
	5 mM	0.8541 mL	4.2704 mL	8.5408 mL	21.3520 mL
	10 mM	0.4270 mL	2.1352 mL	4.2704 mL	10.6760 mL
	15 mM	0.2847 mL	1.4235 mL	2.8469 mL	7.1173 mL
	20 mM	0.2135 mL	1.0676 mL	2.1352 mL	5.3380 mL
	25 mM	0.1708 mL	0.8541 mL	1.7082 mL	4.2704 mL
	30 mM	0.1423 mL	0.7117 mL	1.4235 mL	3.5587 mL
	40 mM	0.1068 mL	0.5338 mL	1.0676 mL	2.6690 mL
	50 mM	0.0854 mL	0.4270 mL	0.8541 mL	2.1352 mL
	60 mM	0.0712 mL	0.3559 mL	0.7117 mL	1.7793 mL
	80 mM	0.0534 mL	0.2669 mL	0.5338 mL	1.3345 mL
	100 mM	0.0427 mL	0.2135 mL	0.4270 mL	1.0676 mL