Art. -Nr.: HY-128729

DNA2 inhibitor C5

Name: DNA2 inhibitor C5
Brand: MedChemExpress
SKU: HY-128729
Price: 71.00 USD
Availability: InStock
Rating: 5 (11 reviews)

11 Cited Publications

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Based on 11 publication(s) in Google Scholar

DNA2 inhibitor C5 is a potent, competitive, and specific DNA2 nuclease inhibitor with an IC₅₀ of 20 μM. DNA2 inhibitor C5 inhibits the nuclease, DNA-dependent ATPase, helicase, and DNA-binding activities of DNA2. DNA2 inhibitor C5 can be used in breast cancer and colorectal cancer research.

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Reinheit : 98.51%
CAS. Nr.: 35973-25-2
Formel: C₁₀H₆N₂O₅
Molecular Weight:234.17

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DNA2 inhibitor C5 Chemical Structure

CAS. Nr.: 35973-25-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll (2)
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Helicase

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	CC₅₀	>100 μM Compound: 2, NSC-15765	Cytotoxicity against human A549 cells after 48 hrs Cytotoxicity against human A549 cells after 48 hrs	[PMID: 20108931]
A549	EC₅₀	>100 μM Compound: 2, NSC-15765	Antiviral activity against Dengue virus type 2 infected in human A549 cells assessed as decrease in viral E protein production by cell-based flavivirus immunodetection assay Antiviral activity against Dengue virus type 2 infected in human A549 cells assessed as decrease in viral E protein production by cell-based flavivirus immunodetection assay	[PMID: 20108931]

In Vitro

DNA2 inhibitor C5 (0-250 μM) inhibits the nuclease activity of DNA2^[1].
DNA2 inhibitor C5 (0-10 μM) has a strong synergistic effect in inhibiting MCF7 cell survival with MK4827, with IC₅₀ values of 0.8 μM and 1.9 μM respectively and a combination index of 0.13^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

DNA2 inhibitor C5 shows synthetic lethality with deficiencies in FEN1, EXO1, or MRE11 genes in the mouse model with HCT116^fox/- cells carrying a conditional genetic knockout of DNA2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 35973-25-2
Appearance Solid
Molecular Weight 234.17
Formel C₁₀H₆N₂O₅
Color Light yellow to yellow
SMILES

O=C(C1=C(O)C2=CC=CC([N+]([O-])=O)=C2N=C1)O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (11)

All Cell Imaging/StainingBio/Physico-chemical AssayIF

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Journal Impact Factor

Nature

Mechanism of BRCA1-BARD1 function in DNA end resection and DNA protection. [Abstract]

2024 Oct;634(8033):492-500. PMID: 39261728

DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Oct;634(8033):492-500. [Abstract]

DNA2 inhibitor C5 (DNA2i) (20 μM; 6 h) reduced DNA end resection in CtIP wild-type cells.

DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Oct;634(8033):492-500. [Abstract]

Quantitation of the cell cycle distribution of the U2OS-derived cell lines treated with the DNA2 inhibitor C5 (DNA2i) (20 μM; 6 h).
Nat Commun

EP300 deficiency leads to chronic replication stress mediated by defective replication fork protection. [Abstract]

2025 Dec 7. PMID: 41354653

DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 7. [Abstract]

DNA2 inhibitor C5 (20 μM; 24 h) significantly reduced ssDNA gap formation in EP300Mut cells.
Nat Commun

DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells. [Abstract]

2025 Aug 25;16(1):7901. PMID: 40854910

DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 25;16(1):7901. [Abstract]

DNA2 inhibitor C5 (100 μM; 30 min). Representative immunofluorescence images and quantification of prometaphase U2OS cells with MiDAS.
Nat Commun

WRN helicase safeguards deprotected replication forks in BRCA2-mutated cancer cells. [Abstract]

2021 Nov 12;12(1):6561. PMID: 34772932
Mol Cell

RADX prevents genome instability by confining replication fork reversal to stalled forks. [Abstract]

2021 Jul 15;81(14):3007-3017.e5. PMID: 34107305

DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2021 Jul 15;81(14):3007-3017.e5. [Abstract]

The fork degradation caused by partial depletion of RADX siRNA was prevented by treatment with DNA2 inhibitor C5 (20 μM).
Nat Chem Biol

Synergistic protection of nascent DNA at stalled forks by MSANTD4 and BRCA1/2-RAD51. [Abstract]

2025 Aug;21(8):1182-1193. PMID: 39809895
Nucleic Acids Res

SNF2L suppresses nascent DNA gap formation to promote DNA synthesis. [Abstract]

2024 Nov 27;52(21):13003-13018. PMID: 39413208
Nucleic Acids Res

Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair. [Abstract]

2024 Oct 14;52(18):10999-11013. PMID: 39180395
Sci Adv

53BP1 orchestrates sequence feature of RAG targets to balance DNA repair outcomes during V(D)J recombination. [Abstract]

2026 May 22;12(21):eaec2231. PMID: 42172328
bioRxiv

ATM promotes reversed fork processing during DNA interstrand cross-link repair. [Abstract]

2025 Oct 9:2025.10.08.680157. PMID: 41279244
bioRxiv

2024 Apr 25.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 33.33 mg/mL (142.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.2704 mL

21.3520 mL

42.7040 mL

5 mM

0.8541 mL

4.2704 mL

8.5408 mL

10 mM

0.4270 mL

2.1352 mL

4.2704 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

All Cell Biology Animals and Diseases

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Binghui Shen, et al. Dna2 inhibitors for cancer treatment. US20170226063A1.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.2704 mL	21.3520 mL	42.7040 mL	106.7600 mL
	5 mM	0.8541 mL	4.2704 mL	8.5408 mL	21.3520 mL
	10 mM	0.4270 mL	2.1352 mL	4.2704 mL	10.6760 mL
	15 mM	0.2847 mL	1.4235 mL	2.8469 mL	7.1173 mL
	20 mM	0.2135 mL	1.0676 mL	2.1352 mL	5.3380 mL
	25 mM	0.1708 mL	0.8541 mL	1.7082 mL	4.2704 mL
	30 mM	0.1423 mL	0.7117 mL	1.4235 mL	3.5587 mL
	40 mM	0.1068 mL	0.5338 mL	1.0676 mL	2.6690 mL
	50 mM	0.0854 mL	0.4270 mL	0.8541 mL	2.1352 mL
	60 mM	0.0712 mL	0.3559 mL	0.7117 mL	1.7793 mL
	80 mM	0.0534 mL	0.2669 mL	0.5338 mL	1.3345 mL
	100 mM	0.0427 mL	0.2135 mL	0.4270 mL	1.0676 mL

Bioz

Keywords

DNA2 inhibitor C5

11 Cited Publications

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DNA2 inhibitor C5

Publications Citing Use of MedChemExpress (MCE) DNA2 inhibitor C5

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