DNA2 inhibitor C5
Based on 11 publication(s) in Google Scholar
DNA2 inhibitor C5 is a potent, competitive, and specific DNA2 nuclease inhibitor with an IC50 of 20 μM. DNA2 inhibitor C5 inhibits the nuclease, DNA-dependent ATPase, helicase, and DNA-binding activities of DNA2. DNA2 inhibitor C5 can be used in breast cancer and colorectal cancer research.
For research use only. We do not sell to patients.
- Purity: 98.51%
- CAS No.: 35973-25-2
- Formula: C10H6N2O5
- Molecular Weight:234.17
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Anti-Breast Cancer Compound Library
HY-L074
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Targeted Diversity Library
HY-L099
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Anti-Colorectal Cancer Compound Library
HY-L103
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Heterocyclic Compound Library
HY-L138
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) DNA2 inhibitor C5
More- Nature. 2024 Oct;634(8033):492-500. [Abstract]
- Nat Commun. 2025 Dec 7. [Abstract]
- Nat Commun. 2025 Aug 25;16(1):7901. [Abstract]
- Nat Commun. 2021 Nov 12;12(1):6561. [Abstract]
- Mol Cell. 2021 Jul 15;81(14):3007-3017.e5. [Abstract]
- Nat Chem Biol. 2025 Aug;21(8):1182-1193. [Abstract]
- Nucleic Acids Res. 2024 Nov 27;52(21):13003-13018. [Abstract]
- Nucleic Acids Res. 2024 Aug 24:gkae721. [Abstract]
- Sci Adv. 2026 May 22;12(21):eaec2231. [Abstract]
- bioRxiv. 2025 Oct 9:2025.10.08.680157. [Abstract]
- bioRxiv. 2024 Apr 25.
Customer Validation & Images
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Bio/Physico-chemical Assay
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IF
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All DNA/RNA Synthesis Isoforms
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Biological Activity
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Helicase |
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Cell Line
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Type | Value | Description | References |
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| A549 | CC50 |
>100 μM
Compound: 2, NSC-15765
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Cytotoxicity against human A549 cells after 48 hrs
Cytotoxicity against human A549 cells after 48 hrs
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[PMID: 20108931] |
| A549 | EC50 |
>100 μM
Compound: 2, NSC-15765
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Antiviral activity against Dengue virus type 2 infected in human A549 cells assessed as decrease in viral E protein production by cell-based flavivirus immunodetection assay
Antiviral activity against Dengue virus type 2 infected in human A549 cells assessed as decrease in viral E protein production by cell-based flavivirus immunodetection assay
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[PMID: 20108931] |
DNA2 inhibitor C5 (0-250 μM) inhibits the nuclease activity of DNA2[1].
DNA2 inhibitor C5 (0-10 μM) has a strong synergistic effect in inhibiting MCF7 cell survival with MK4827, with IC50 values of 0.8 μM and 1.9 μM respectively and a combination index of 0.13[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 35973-25-2
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Appearance Solid
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Molecular Weight 234.17
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Formula C10H6N2O5
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Color Light yellow to yellow
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SMILES
O=C(C1=C(O)C2=CC=CC([N+]([O-])=O)=C2N=C1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (11)
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Journal Impact Factor
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Most Recent
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Nature
2024 Oct;634(8033):492-500. PMID: 39261728
DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Oct;634(8033):492-500. [Abstract]
DNA2 inhibitor C5 (DNA2i) (20 μM; 6 h) reduced DNA end resection in CtIP wild-type cells.
DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Oct;634(8033):492-500. [Abstract]
Quantitation of the cell cycle distribution of the U2OS-derived cell lines treated with the DNA2 inhibitor C5 (DNA2i) (20 μM; 6 h).
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Nat Commun
EP300 deficiency leads to chronic replication stress mediated by defective replication fork protection. [Abstract]2025 Dec 7. PMID: 41354653
DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Dec 7. [Abstract]
DNA2 inhibitor C5 (20 μM; 24 h) significantly reduced ssDNA gap formation in EP300Mut cells.
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Nat Commun
DNA double-strand break end resection factors and WRN facilitate mitotic DNA synthesis in human cells. [Abstract]2025 Aug 25;16(1):7901. PMID: 40854910
DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 25;16(1):7901. [Abstract]
DNA2 inhibitor C5 (100 μM; 30 min). Representative immunofluorescence images and quantification of prometaphase U2OS cells with MiDAS.
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Nat Commun
2021 Nov 12;12(1):6561. PMID: 34772932 -
Mol Cell
RADX prevents genome instability by confining replication fork reversal to stalled forks. [Abstract]2021 Jul 15;81(14):3007-3017.e5. PMID: 34107305
DNA2 inhibitor C5 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2021 Jul 15;81(14):3007-3017.e5. [Abstract]
The fork degradation caused by partial depletion of RADX siRNA was prevented by treatment with DNA2 inhibitor C5 (20 μM).
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Nat Chem Biol
2025 Aug;21(8):1182-1193. PMID: 39809895 -
Nucleic Acids Res
2024 Nov 27;52(21):13003-13018. PMID: 39413208 -
Nucleic Acids Res
Replication fork stalling in late S-phase elicits nascent strand degradation by DNA mismatch repair. [Abstract]2024 Aug 24:gkae721. PMID: 39180395 -
Sci Adv
53BP1 orchestrates sequence feature of RAG targets to balance DNA repair outcomes during V(D)J recombination. [Abstract]2026 May 22;12(21):eaec2231. PMID: 42172328 -
bioRxiv
2025 Oct 9:2025.10.08.680157. PMID: 41279244 -
Solvent & Solubility
DMSO : 33.33 mg/mL (142.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.2704 mL | 21.3520 mL | 42.7040 mL | 106.7600 mL |
| 5 mM | 0.8541 mL | 4.2704 mL | 8.5408 mL | 21.3520 mL | |
| 10 mM | 0.4270 mL | 2.1352 mL | 4.2704 mL | 10.6760 mL | |
| 15 mM | 0.2847 mL | 1.4235 mL | 2.8469 mL | 7.1173 mL | |
| 20 mM | 0.2135 mL | 1.0676 mL | 2.1352 mL | 5.3380 mL | |
| 25 mM | 0.1708 mL | 0.8541 mL | 1.7082 mL | 4.2704 mL | |
| 30 mM | 0.1423 mL | 0.7117 mL | 1.4235 mL | 3.5587 mL | |
| 40 mM | 0.1068 mL | 0.5338 mL | 1.0676 mL | 2.6690 mL | |
| 50 mM | 0.0854 mL | 0.4270 mL | 0.8541 mL | 2.1352 mL | |
| 60 mM | 0.0712 mL | 0.3559 mL | 0.7117 mL | 1.7793 mL | |
| 80 mM | 0.0534 mL | 0.2669 mL | 0.5338 mL | 1.3345 mL | |
| 100 mM | 0.0427 mL | 0.2135 mL | 0.4270 mL | 1.0676 mL |
DNA2 inhibitor C5 Related Classifications
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