Dnp-PLGLWA-DArg-NH2 TFA
Based on 1 Customer Validation
Dnp-PLGLWA-DArg-NH2 TFA is a fluorogenic substrate for MMP-1 and MMP-9. Dnp-PLGLWA-DArg-NH2 TFA can be used to quantify the activity of MMPs (Ex=280 nm, Em=360 nm).
For research use only. We do not sell to patients.
- Purity: 99.78%
- Formula: C47H65F3N14O13
- Molecular Weight:1091.10
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
MMP-1 |
MMP-9 |
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Enzyme activity assays are performed in 50 mM Tris-HCl buffer, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% TNC buffer containing 0.05% Brij 35 and 50 μM ZnSO4.
2. Dnp-PLGLWA-DArg-NH2 TFA. Each fraction is incubated with 1 μM substrate at 37°C for 20 h.
3. Stop the reaction by the addition of 3% acetic acid.
4. Measure the fluorescence using wavelengths of 280 nm (excitation) and 360 nm (emission) with a fluorescence reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
Appearance Solid
-
Molecular Weight 1091.10
-
Formula C47H65F3N14O13
-
Color Light yellow to yellow
-
Sequence
{Dnp}-Pro-Leu-Gly-Leu-Trp-Ala-{dArg}-NH2
-
Sequence Shortening
{Dnp}-PLGLWA-{dArg}-NH2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (91.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. G M McGeehan, et al. Characterization of the peptide substrate specificities of interstitial collagenase and 92-kDa gelatinase. Implications for substrate optimization. J Biol Chem. 1994 Dec 30;269(52):32814-20. [Content Brief]
[2]. Ken-ichi Shimokawa Ki, et al. Matrix metalloproteinase (MMP)-2 and MMP-9 activities in human seminal plasma. Mol Hum Reprod. 2002 Jan;8(1):32-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9165 mL | 4.5825 mL | 9.1651 mL | 22.9127 mL |
| 5 mM | 0.1833 mL | 0.9165 mL | 1.8330 mL | 4.5825 mL | |
| 10 mM | 0.0917 mL | 0.4583 mL | 0.9165 mL | 2.2913 mL | |
| 15 mM | 0.0611 mL | 0.3055 mL | 0.6110 mL | 1.5275 mL | |
| 20 mM | 0.0458 mL | 0.2291 mL | 0.4583 mL | 1.1456 mL | |
| 25 mM | 0.0367 mL | 0.1833 mL | 0.3666 mL | 0.9165 mL | |
| 30 mM | 0.0306 mL | 0.1528 mL | 0.3055 mL | 0.7638 mL | |
| 40 mM | 0.0229 mL | 0.1146 mL | 0.2291 mL | 0.5728 mL | |
| 50 mM | 0.0183 mL | 0.0917 mL | 0.1833 mL | 0.4583 mL | |
| 60 mM | 0.0153 mL | 0.0764 mL | 0.1528 mL | 0.3819 mL | |
| 80 mM | 0.0115 mL | 0.0573 mL | 0.1146 mL | 0.2864 mL |
Dnp-PLGLWA-DArg-NH2 TFA Related Classifications
HY-P3484 Related Classifications
Powered by Bioz