Driselase, Basidiomycetes sp
Based on 1 Customer Validation
Driselase, Basidiomycetes sp, a complex mixt. of wall-digesting enzymes, is a specific commercial fungal protoplasting enzyme preparation. Driselase can be used in combination with lyase to promote protoplast formation in fungi. Driselase is by far the most potent of the enzymes tested for polysaccharide digestion and greatly increases both tensile and indentation compliances, yet it does not induce wall creep, even after 6 h of digestion.
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- CAS No.: 85186-71-6
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Product Information Crashase is a cell wall degrading enzyme composed of cellulase, hemicellulase, pectinase, etc. It is a crude enzyme that can be used to remove plant cell walls to produce protoplasts. Instructions Soluble in water (1 mg/mL) and is a clear to slightly turbid liquid after dissolution. Or dissolve according to specific experimental references
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.2.1.23
CellUlase:≥100 U/mg soild Laminarinase:≥
Cellulase:One unit will liberate 1.0 μmol of glucose from cellulose in 1 h at pH 5.0 at 37°C. Laminarinase:One unit will liberate 1.0 mg of reducing sugar (as glucose) from laminarin per minute at pH 5.0 at 37°C. Xylanase:One unit will liberate 1.0 mg of reducing sugar (as glucose) from xylan per minute at pH 4.5 at 37°C
Chemical Information
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CAS No. 85186-71-6
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Appearance Solid
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Color White to yellow
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SMILES
[Driselase, Basidiomycetes sp]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (264 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Ramamoorthy V, et al. Combination of driselase and lysing enzyme in one molar potassium chloride is effective for the production of protoplasts from germinated conidia of Fusarium verticillioides. J Microbiol Methods. 2015 Apr;111:127-34. [Content Brief]
[2]. Ramamoorthy V, et al. Combination of driselase and lysing enzyme in one molar potassium chloride is effective for the production of protoplasts from germinated conidia of Fusarium verticillioides. J Microbiol Methods. 2015 Apr;111:127-34. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)