Other Indicator Probes

Copper Fluor-4 (solution) (CF4 (solution)) is a Cu⁺-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 solution has high copper selectivity with a K_d value of 2.9×10^?13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 solution is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).
Solvent and concentration: DMSO: 5 mM

Methylthymol blue tetrasodium is a chelating agent and metal chromogenic indicator that exhibits a significant color change upon reaction with certain metal cations. Methylthymol blue tetrasodium can be used for the determination of metal ions such as sulfate and calcium ions.

Ctrl-BODIPYS1 is a BODIPY-based control probe that shows no response to copper ions (exhibiting two emission peaks at 575 nm and 708 nm; the maximum excitation wavelength is at 627 nm). Ctrl-BODIPYS1 has no fluorescent response to Cu⁺.

BODIPYS1 is a near-infrared ratiometric fluorescent sensor and labile copper detection probe. BODIPYS1 possesses excitation-ratiometric copper-sensing capability, with the ratio of near-infrared emission under 600 nm excitation (710 nm) to that under 660 nm excitation, F_ex600/F_ex660, increasing from 2.8 to 10.7. BODIPYS1 is capable of tracking labile copper ions in mice.

DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λ_ex=480 nm, λ_em=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group.

FLCS1 is a BODIPY-based Fluorescent probe and selective copper (I) ion binder (λ_ex = 630 nm, λ_emis = 660 nm). Binding of FLCS1 to copper (I) ions induces fluorescence turn-on. Addition of copper (I) to a methanolic solution of FLCS1 causes a slight red shift (4 nm) in the maximum absorption wavelength (λ_max) in the UV-Vis spectrum.

NIR-Cu (Ⅱ) is a near-infrared fluorescent Cu²⁺ probe. NIR-Cu (Ⅱ) enables fluorescence imaging of Cu²⁺ in living mice. NIR-Cu (Ⅱ) reduces self-absorption and exhibits minimal photobleaching, thus supporting in vivo imaging applications.

Mito-Cu (II) is a mitochondria-targeted fluorescent probe (Ex/Em = 370/450 nM). Mito-Cu (II) specifically accumulates in mitochondria of living cells and enables real-time detection of exogenous Cu²⁺ within mitochondria of living cells. Mito-Cu (II) achieves "on-off-on" fluorescence switching through sequential exposure to Cu²⁺ and ethylenediaminetetraacetic acid (EDTA) (HY-Y0682). Its fluorescence is quenched after forming a 1:1 complex with Cu²⁺, and the fluorescence recovers when Cu²⁺ is chelated by EDTA.

Cobalt (II) plant dye (Compound 3) is a Cobalt (II) probe. Cobalt (II) plant dye is used for the selective detection of bioaccumulated Cobalt (II) ions in root and stem tissues of Hybanthus enneaspermus plants.

CSF1 is a silicon-rhodamine-based, fluorescence-activatable probe with selectivity for Cu⁺. CSF1 colocalizes with the endoplasmic reticulum in live cells.

Ratio-Coppersensor-1 is a selective ratiometric copper ion Fluorescent reporter (excitation wavelength: 480 nm; emission peak central wavelengths at 505 nm and 570 nm, respectively). Ratio-Coppersensor-1 exhibits extremely high selectivity for Cu⁺ at cellular concentrations, outperforming other competing metal ions, and shows an approximately 20-fold change in fluorescence ratio upon Cu⁺ binding. Ratio-Coppersensor-1 is capable of reflecting changes in Cu levels.

ER-Cu (II) is an endoplasmic reticulum-selective fluorescent Cu²⁺ probe. ER-Cu (II) localizes specifically to the endoplasmic reticulum of hepatocytes.

RDDB is a rhodamine-based turn-on fluorescent and colorimetric chemosensor selective for Mn²⁺, with a limit of detection of 5×10^-8 M (excitation wavelength: 480-550 nm; fluorescence signal collection range: >590 nm). In the presence of Mn²⁺, RDDB undergoes a spirolactam ring-opening reaction of its rhodamine hydrazide moiety, resulting in turn-on fluorescence and a color change. RDDB can be used for intracellular Mn²⁺ imaging.

Ctrl-CPF1 is a control dye for Copper Phosphorus Fluor 1 (CPF1), which exhibits no metal-dependent fluorescence response and shows no reactivity toward Cu⁺ .

DHUCu-1 is a lysosome-targeted NIR divalent copper ion probe with high water solubility. DHUCu-1 serves as a tool for investigating the physiological functions of Cu²⁺ and related diseases, such as Menke syndrome, Wilson's disease, and Alzheimer's disease.