EB1 peptide  (Synonyms: Penetrating analog)

EB1 peptide (Penetrating analog) is an endosomolytic agent and siRNA delivery agent. EB1 peptide forms an amphipathic alpha helix upon protonation in early-late endosomes, drives endosomal membrane permeabilization, and enables endocytosed siRNA escape into the cytosol. EB1 peptide facilitates biologically active siRNA cellular uptake and targeted gene silencing. EB1 peptide forms complexes with siRNA. EB1 peptide can be used for drug delivery research^[1].

EB1 peptide (10:1-25:1 molar ratio of EB1 peptide: siRNA; 1 h) delivers significantly more siRNA into HeLa cells at low molar ratios, with 10-fold higher uptake at 10:1 and 2.5-fold higher uptake at 25:1 compared to Penetratin (HY-P2529)^[1].
EB1 peptide (25:1 molar ratio of EB1 peptide: siRNA; 36 h) delivers biologically active siRNA to transiently transfected HeLa cells, significantly reducing luciferase expression to ~50% of control at 100 nM siRNA in both serum-free and serum-containing conditions, and to ~70% of control at 10 nM siRNA in serum-free conditions^[1].
EB1 peptide (25:1 molar ratio of EB1 peptide: siRNA; 48 h) delivers biologically active siRNA to stably transfected HepG2 cells when preincubated in a large volume, significantly reducing luciferase expression to ~65% of control at 100 nM siRNA after 48 h^[1].
EB1 peptide (25:1 molar ratio of EB1 peptide: siRNA; 36 h) promotes more effective endosomal escape of siRNA in transiently transfected HeLa cells than HA2-penetratin-augmented Penetratin, resulting in greater luciferase gene silencing at a 25:1 peptide: siRNA ratio after 36 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3099.78

C₁₅₀H₂₃₆N₄₆O₂₆

952754-66-4

Solid

White to off-white

Leu-Ile-Arg-Leu-Trp-Ser-His-Leu-Ile-His-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Leu-Lys-Trp-Lys-Lys-Lys-NH2

LIRLWSHLIHIWFQNRRLKWKKK-NH2

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Lundberg P, et al. Delivery of short interfering RNA using endosomolytic cell-penetrating peptides. FASEB J. 2007 Sep;21(11):2664-71.