Euphomianol A
Euphomianol A is a modified ent-rosane diterpenoid with a 10,19-oxygen bridge that can be found in the aerial parts of Euphorbia milii. Euphomianol A shows no inhibitory effect on RANKL-induced osteoclastogenesis in macrophages.
For research use only. We do not sell to patients.
- CAS No.: 2077182-81-9
- Formula: C20H32O3
- Molecular Weight:320.47
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Euphomianol A is inactive as an inhibitor of RANKL-induced osteoclast formation in RAW 264.7 cells at the concentrations tested[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2077182-81-9
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Molecular Weight 320.47
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Formula C20H32O3
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SMILES
O[C@@]12[C@@]3([C@]4(C)[C@@](CC1)(C[C@](C=C)(C)CC4)[H])OC[C@]2(C)[C@@H](O)CC3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Osteoclast differentiation from monocyte/macrophage precursors
Osteoclast differentiation is an in vitro induction assay in which monocyte/macrophage-lineage precursors are exposed to macrophage colony-stimulating factor (M-CSF) and receptor activator of NF-κB ligand (RANKL), generating multinucleated osteoclasts that are commonly identified by tartrate-resistant acid phosphatase (TRAP) staining and functionally confirmed by resorption pits on dentin, bone, or mineralized substrates. M-CSF supports survival and expansion of osteoclast precursors, while RANKL binding to RANK drives osteoclast commitment, fusion, maturation, and resorptive function; osteoprotegerin inhibits this pathway by binding RANKL and preventing RANK activation. The main readouts are the number of TRAP-positive multinucleated cells, formation of F-actin rings, and resorbed surface area; TRAP-positive multinucleated cells indicate osteoclast differentiation, whereas pit formation on dentin, bone, or mineralized coating indicates functional bone-resorbing activity.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Euphomianol A Related Classifications
HY-N21566 Related Classifications
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