2. GPCR/G Protein
  3. Taste Receptor
  4. FEMA 4774

FEMA 4774 is a positive allosteric modulator of taste receptors T1R2 and T1R3, two subunits of the human sweet taste receptor. FEMA 4774 is also used as a sucrose sweetness enhancer.

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FEMA 4774 Chemical Structure

FEMA 4774 Chemical Structure

CAS No. : 1359963-68-0

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Based on 1 publication(s) in Google Scholar

FEMA 4774 Related Antibodies

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Description

FEMA 4774 is a positive allosteric modulator of taste receptors T1R2 and T1R3, two subunits of the human sweet taste receptor. FEMA 4774 is also used as a sucrose sweetness enhancer[1].

In Vitro

FEMA 4774 (0-50 μM) induces a dose-dependent decrease in the EC50 value of sucrose and significantly enhances the affinity of sucrose for its binding site with an α (effect of modulator on agonist affinity) value of 30 in the human sweet receptor cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FEMA 4774 Related Antibodies
In Vivo

FEMA 4774 (S9632) (oral gavage, 500-2000 mg/kg) does not cause a dose-dependent increase in polychromatic erythrocytes and induces micronuclei formation at dose levels up to 2000 mg/kg in Swiss albino (CD-1) mice[2].
The pharmacokinetic parameters of FEMA 4774 (S9632) in Male and Female Sprague-Dawley Rats

Route Day Dose (mg/kg) Sex Cmax (ng/mL)±SD Tmax (hr) t1/2 (hr) AUC0last(ng•hr/mL) AUC0last/dose(ng•hr/mL/mg/kg) %F
iv 1 1.0 M 2036.7±281.9 0.03 0.19 330.6±58 330.6 -
F 2625.8±679.9 0.03 0.27 411.9±116.3 411.9 -
Oral gavage 1 10 M 12.9±3.2 0.25 1.21 17.9±2.3 1.79 0.54
F 34.0±3.9 0.25 1.17 49.2±7.8 4.92 1.19
30 M 90.0±23.9 0.33 0.88 90.8±11.0 3.03 0.92
F 62.4±25.9 0.42 1.06 95.0±20.4 3.17 0.77
100 M 147.2±86.3 0.33 0.71 176.2±73.8 1.76 0.53
F 268.5±90.7 0.25 0.99 341.1±81.0 3.41 0.83
Oral gavage 7 10 M 16.2±3.6 0.50 0.84 48.1±33.2 4.81 -
F 32.7±9.7 0.33 1.17 58.9±25.4 5.89 -
30 M 74.5±16.2 0.33 0.94 86.0±8.7 2.87 -
F 77.1±41.7 0.25 1.39 106.8±8.7 3.56 -
100 M 117.9±15.3 0.25 0.72 185.3±15.3 1.85 -
F 189.7±79.5 0.25 0.89 278.9±79.0 2.79 -

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

359.42

Formula

C19H25N3O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C(C2=C(OCC(C)(C)C(NC(C)C)=O)C=CC=C2N=C1C)N)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 41.67 mg/mL (115.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7823 mL 13.9113 mL 27.8226 mL
5 mM 0.5565 mL 2.7823 mL 5.5645 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7823 mL 13.9113 mL 27.8226 mL 69.5565 mL
5 mM 0.5565 mL 2.7823 mL 5.5645 mL 13.9113 mL
10 mM 0.2782 mL 1.3911 mL 2.7823 mL 6.9557 mL
15 mM 0.1855 mL 0.9274 mL 1.8548 mL 4.6371 mL
20 mM 0.1391 mL 0.6956 mL 1.3911 mL 3.4778 mL
25 mM 0.1113 mL 0.5565 mL 1.1129 mL 2.7823 mL
30 mM 0.0927 mL 0.4637 mL 0.9274 mL 2.3186 mL
40 mM 0.0696 mL 0.3478 mL 0.6956 mL 1.7389 mL
50 mM 0.0556 mL 0.2782 mL 0.5565 mL 1.3911 mL
60 mM 0.0464 mL 0.2319 mL 0.4637 mL 1.1593 mL
80 mM 0.0348 mL 0.1739 mL 0.3478 mL 0.8695 mL
100 mM 0.0278 mL 0.1391 mL 0.2782 mL 0.6956 mL
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FEMA 4774 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FEMA 47741359963-68-0FEMA4774FEMA-4774Taste Receptorweetness enhancerpositive allosteric modulatorT1R2T1R3sucroseInhibitorinhibitorinhibit

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