FLT3-IN-24
FLT3-IN-24 (compound 24) is a potent and selective inhibitor of FLT3 kinase with an IC50 of 7.94 nM. FLT3-IN-24 has cell antiproliferative effects.
For research use only. We do not sell to patients.
- Formula: C23H20N6O2S
- Molecular Weight:444.51
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
>1000 nM
Compound: 24
|
Cytotoxicity against HEK293 cells lacking FLT3 activating mutations assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
Cytotoxicity against HEK293 cells lacking FLT3 activating mutations assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
|
[PMID: 38056299] |
| K562 | IC50 |
>1000 nM
Compound: 24
|
Antiproliferative activity against human K562 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
Antiproliferative activity against human K562 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
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[PMID: 38056299] |
| MDA-MB-231 | IC50 |
>1000 nM
Compound: 24
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Antiproliferative activity against human MDA-MB-231 cells lacking FLT3 activating mutations assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
Antiproliferative activity against human MDA-MB-231 cells lacking FLT3 activating mutations assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
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[PMID: 38056299] |
| MOLM-14 | IC50 |
3.16 nM
Compound: 24
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Antiproliferative activity against human MOLM-14 cells harboring FLT3-D835Y mutant assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
Antiproliferative activity against human MOLM-14 cells harboring FLT3-D835Y mutant assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
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[PMID: 38056299] |
| MOLM-14 | IC50 |
4.55 nM
Compound: 24
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Antiproliferative activity against human MOLM-14 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
Antiproliferative activity against human MOLM-14 cells assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
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[PMID: 38056299] |
| MOLM-14 | IC50 |
8.34 nM
Compound: 24
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Antiproliferative activity against human MOLM-14 cells harboring FLT3-F691L mutant assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
Antiproliferative activity against human MOLM-14 cells harboring FLT3-F691L mutant assessed as inhibition of cell growth incubated for 72 hrs by resazurin dye based assay
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[PMID: 38056299] |
Chemical Information
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Molecular Weight 444.51
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Formula C23H20N6O2S
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SMILES
CS(=O)(C1=CC=C(C=C1)NC2=CC=CC(C3=CN=C4C=C(C=CN34)C5=CN(C)N=C5)=N2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
FLT3-IN-24 Related Classifications
HY-163068 Related Classifications
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