FLT3-IN-32 TFA
FLT3-IN-32 TFA is a potent FLT3 inhibitor with an IC50s of 2.40 nM and 3.83 nM against FLT3-ITD and FLT3-D835Y. FLT3-IN-32 TFA inhibits proliferation/survival of human MV4-11 cells with an IC50 of 0.07 nM. FLT3-IN-32 TFA can be used for the study of acute myeloid leukemia (AML).
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- No. CAS: 3047195-49-0
- Fòrmula: C30H30F3N5O7
- Peso molecular:629.58
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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- Pureza y Documentación
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Help & FAQs
Actividad biológica
Descripciòn
In Vitro
FLT3-IN-32 TFA (Compound 29b) exhibits antiproliferative activity against EOL-1 (IC50 = 0.02 nM), MOLM-13 (IC50 = 0.20 nM), MONO-MAC-1 (IC50 = 100 nM) and MONO-MAC-6 (IC50 = 0.65 nM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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No. CAS 3047195-49-0
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Peso molecular 629.58
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Fòrmula C30H30F3N5O7
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SMILES
O=C(NC1=CC2=C(OC(C(C3=CC4=C(CN(C)C)C(O)=CC=C4N3)=O)=C2)C=C1)NC5=NOC(C(C)(C)C)=C5.O=C(O)C(F)(F)F
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
FLT3-IN-32 TFA Related Classifications
HY-174437A Related Classifications
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