Fluorescent Brightener 28
Based on 1 publication(s) in Google Scholar
Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 4193-55-9
- Formula: C40H42N12Na2O10S2
- Molecular Weight:960.95
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fluorescent Brightener 28
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Biological Activity
Fluorescent Brightener 28 (8.1%) stains nuclei in pupal wing epithelial tissues (blue)[2]. Fluorescent Brightener 28-coated Fe3O4 nanoparticles shows a strong fluorescence maximum (λ max) at 435 nm[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4193-55-9
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Appearance Solid
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Molecular Weight 960.95
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Formula C40H42N12Na2O10S2
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Color Light yellow to yellow
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SMILES
O=S(C1=CC(NC2=NC(NC3=CC=CC=C3)=NC(N(CCO)CCO)=N2)=CC=C1/C=C/C4=CC=C(NC5=NC(NC6=CC=CC=C6)=NC(N(CCO)CCO)=N5)C=C4S(=O)(O[Na])=O)(O[Na])=O
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Synonyms
Calcofluor White M2R
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Plant Physiol
Expansin-like A binds to pectin via electrostatic forces and remodels the plant cell wall. [Abstract]2026 Feb 6;200(2):kiag029. PMID: 41612931
Solvent & Solubility
DMSO : 100 mg/mL (104.06 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (52.03 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[2]. Nakazato Y,et al. Live Detection of Intracellular Chitin in Butterfly Wing Epithelial Cells In Vivo Using Fluorescent Brightener 28: Implications for the Development of Scales and Color Patterns. Insects. 2023 Sep 8;14(9):753. [Content Brief]
[3]. Xiaoling Zuo, et al. Fluorescent Brighteners as Visible LED-Light Sensitive Photoinitiators for Free Radical Photopolymerizations. Macromol Rapid Commun. 2016 May;37(10):840-4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.0406 mL | 5.2032 mL | 10.4064 mL | 26.0159 mL |
| 5 mM | 0.2081 mL | 1.0406 mL | 2.0813 mL | 5.2032 mL | |
| 10 mM | 0.1041 mL | 0.5203 mL | 1.0406 mL | 2.6016 mL | |
| 15 mM | 0.0694 mL | 0.3469 mL | 0.6938 mL | 1.7344 mL | |
| 20 mM | 0.0520 mL | 0.2602 mL | 0.5203 mL | 1.3008 mL | |
| 25 mM | 0.0416 mL | 0.2081 mL | 0.4163 mL | 1.0406 mL | |
| 30 mM | 0.0347 mL | 0.1734 mL | 0.3469 mL | 0.8672 mL | |
| 40 mM | 0.0260 mL | 0.1301 mL | 0.2602 mL | 0.6504 mL | |
| 50 mM | 0.0208 mL | 0.1041 mL | 0.2081 mL | 0.5203 mL | |
| DMSO | 60 mM | 0.0173 mL | 0.0867 mL | 0.1734 mL | 0.4336 mL |
| 80 mM | 0.0130 mL | 0.0650 mL | 0.1301 mL | 0.3252 mL | |
| 100 mM | 0.0104 mL | 0.0520 mL | 0.1041 mL | 0.2602 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Fluorescent Brightener 28 Related Classifications
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