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Fluorescent Brightener 28  (Synonyms: Calcofluor White M2R)

Cat. No.: HY-D0367 Purity: 93.20%
COA
SDS
Handling Instructions Technical Support

Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations.

For research use only. We do not sell to patients.

Fluorescent Brightener 28

Fluorescent Brightener 28 Chemical Structure

CAS No. : 4193-55-9

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Fluorescent Brightener 28:

Fluorescent Brightener 28 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Fluorescent Brightener 28

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Fluorescent brightener 28 is a fluorescent whitening agent commonly used in the padding process of the textile industry. Fluorescent brightener 28 is capable of staining polysaccharides such as cellulose, and when the plasma membrane ruptures, it also weakly stains the cytoplasm and strongly stains the cell nucleus. Additionally, Fluorescent brightener 28 can be utilized to detect intracellular chitin in living cells. Fluorescent Brightener 28 also is a visible light emitting diode (LED)-light sensitive photoinitiator for free radical photopolymerizations[1][2][3].

In Vitro

Fluorescent Brightener 28 (8.1%) stains nuclei in pupal wing epithelial tissues (blue)[2].
Fluorescent Brightener 28-coated Fe3O4 nanoparticles shows a strong fluorescence maximum (λ max) at 435 nm[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescent Brightener 28 Related Antibodies
Molecular Weight

960.95

Formula

C40H42N12Na2O10S2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC(NC2=NC(NC3=CC=CC=C3)=NC(N(CCO)CCO)=N2)=CC=C1/C=C/C4=CC=C(NC5=NC(NC6=CC=CC=C6)=NC(N(CCO)CCO)=N5)C=C4S(=O)(O[Na])=O)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (156.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (52.03 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0406 mL 5.2032 mL 10.4064 mL
5 mM 0.2081 mL 1.0406 mL 2.0813 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 93.20%

SDS (393 KB)

COA (251 KB) HNMR (381 KB) RP-HPLC (250 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.0406 mL 5.2032 mL 10.4064 mL 26.0159 mL
5 mM 0.2081 mL 1.0406 mL 2.0813 mL 5.2032 mL
10 mM 0.1041 mL 0.5203 mL 1.0406 mL 2.6016 mL
15 mM 0.0694 mL 0.3469 mL 0.6938 mL 1.7344 mL
20 mM 0.0520 mL 0.2602 mL 0.5203 mL 1.3008 mL
25 mM 0.0416 mL 0.2081 mL 0.4163 mL 1.0406 mL
30 mM 0.0347 mL 0.1734 mL 0.3469 mL 0.8672 mL
40 mM 0.0260 mL 0.1301 mL 0.2602 mL 0.6504 mL
50 mM 0.0208 mL 0.1041 mL 0.2081 mL 0.5203 mL
DMSO 60 mM 0.0173 mL 0.0867 mL 0.1734 mL 0.4336 mL
80 mM 0.0130 mL 0.0650 mL 0.1301 mL 0.3252 mL
100 mM 0.0104 mL 0.0520 mL 0.1041 mL 0.2602 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Fluorescent Brightener 28 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescent Brightener 284193-55-9Calcofluor White M2RFluorescent Brightener28Fluorescent Brightener-28Fluorescent Dyefree radical photopolymerizationlight emitting diodeLEDphotoinitiatorpadding processchitindyeingtextile industryInhibitorinhibitorinhibit

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