Cat. No.: HY-B0166

L-Ascorbic acid

(Synonyms: L-Ascorbate; Vitamin C)

81 Cited Publications

Customer Review

Based on 81 publication(s) in Google Scholar

L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Ca_v3.2 channels with an IC₅₀ of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 50-81-7
Formula: C₆H₈O₆
Molecular Weight:176.12

Storage:

RT, protect from light.

In solvent -80°C, 1 year , -20°C, 6 months

L-Ascorbic acid Chemical Structure

CAS No.: 50-81-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (81)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Ca_v3.2 channels with an IC₅₀ of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor^[1]^[2]^[3]. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells^[4].

IC₅₀ & Target

T-type calcium channel

Microbial Metabolite

All Calcium Channel Isoforms :

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
CFPAC-1	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human CFPAC-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human CFPAC-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
Erythrocyte	EC₅₀	119.87 μg/mL Compound: Ascorbic acid	Antioxidant activity in erythrocytes assessed as inhibition of hemolysis Antioxidant activity in erythrocytes assessed as inhibition of hemolysis	[PMID: 22677320]
Erythrocyte	EC₅₀	96.625 μg/mL Compound: Ascorbic acid	Hemolysis of human erythrocytes after 30 mins by spectrophotometry Hemolysis of human erythrocytes after 30 mins by spectrophotometry	[PMID: 23811092]
HCT-116	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human HCT116cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT116cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
HEK293	EC₅₀	0.5 nM Compound: Ascorbic acid	Agonist activity at human adrenergic alpha2B receptor expressed in HEK293 cells coexpressing Gqi5 protein by FLIPR assay Agonist activity at human adrenergic alpha2B receptor expressed in HEK293 cells coexpressing Gqi5 protein by FLIPR assay	[PMID: 19243956]
HEK293	EC₅₀	156 μM Compound: 54670067	Substrate uptake and inhibition of the Na(+)/L-Ascorbic Acid Transporter 1 (SVCT1, SLC23A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC23A1 cells (PubChem AID: 1794810) Substrate uptake and inhibition of the Na(+)/L-Ascorbic Acid Transporter 1 (SVCT1, SLC23A1) as assessed by the fluorescent FLIPR membrane potential dye in HEK-293 JumpIN-SLC23A1 cells (PubChem AID: 1794810)	10.5281/zenodo.7360349
HeLa	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
HepG2	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
HFF	IC₅₀	>100 μM Compound: L-ASA	Cytotoxicity against human HFF cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HFF cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
HL-60	IC₅₀	0.01 μM Compound: Vitamin C	Antioxidant activity in human HL60 cells assessed as inhibition of relative oxygen species generation by DCFH-DA method Antioxidant activity in human HL60 cells assessed as inhibition of relative oxygen species generation by DCFH-DA method	[PMID: 19555121]
HL-60	IC₅₀	10.2 μM Compound: Vitamin C	Antioxidant activity in human HL60 cells assessed as inhibition of TPA-stimulated hydrogen peroxide induced DCFH-DA oxidation by fluorometric microplate assay Antioxidant activity in human HL60 cells assessed as inhibition of TPA-stimulated hydrogen peroxide induced DCFH-DA oxidation by fluorometric microplate assay	[PMID: 16562834]
HL-60	IC₅₀	9.7 μM Compound: Vitamin C	Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay Antioxidant activity against TPA-induced ROS production in human HL60 cells by 2',7'-dichlorodihydrofluorescein diacetate cellular-based assay	[PMID: 12762791]
J774.A1	IC₅₀	>100 μM Compound: AA	Antiinflammatory activity in mouse J774.A1 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis Antiinflammatory activity in mouse J774.A1 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis	[PMID: 33422907]
MCF7	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]
RD	EC₅₀	>2270 μM Compound: AA	Antiviral activity against Herpes simplex virus type 1 infected in human RD cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against Herpes simplex virus type 1 infected in human RD cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 22390834]
SW-620	IC₅₀	>100 μM Compound: L-ASA	Antiproliferative activity against human SW620 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human SW620 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31586832]

In Vitro

The anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:

High SVCT-2 expressing cell lines Sw620, Sw480, LoVo, SNU-C4; Low SVCT-2 expressing cell lines HCT15, HCT116, DLD-1, CoLo-205
Concentration:

0, 0.1 μM, 1 μM, 10 μM, 0.1 mM, 0.5 mM, 1 mM, and 2 mM
Incubation Time:

24 hours
Result:

Some high SVCT-2 expressing cancer cells demonstrated a dramatic cell-autonomous inhibitory effect of L-ascorbic acid.
Low SVCT-2 expressing cell lines showed biphasic responses to L-ascorbic acid.

Western Blot Analysis^[4]

Cell Line:

Sw620, Sw480, LoVo, SNU-C4, HCT15, HCT116, DLD-1, CoLo-205 cell lines
Concentration:

1 mM
Incubation Time:
Result:

The cell lines showed different levels of SVCT-2 expression in western blot analyses: Sw620, Sw480, and Lovo expressed high levels of SVCT-2 whereas HCT116, HCT15, and DLD-1 expressed low levels.

In Vivo

L-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Normal rats:Albino rats of either sex weighing between 125-175 g^[5]
Dosage:

Group I received L-ascorbic acid 60 mg/kg, Group II received Tolbutamide 20 mg/kg and Group III was given L-ascorbic acid (60 mg/kg) prior to the administration of tolbutamide 20 mg/kg
Administration:

Administered orally
Result:

L-ascorbic acid at the dose of 60 mg/kg produced 50.91% blood glucose reduction at 0.5 h and 20 mg/kg body weight of Tolbutamide produced 33% at 4 h as peak effects. In the presence of L-ascorbic acid (60 mg/kg), the action of Tolbutamide was early in onset and maintained for 6 h.

Animal Model:

Diabetic rats:Albino rats of either sex weighing between 125 to 175 g were fasted overnight before injection with Alloxan^[5]
Dosage:

Group I received L-ascorbic acid 40 mg/kg and Group II received Tolbutamide 20 mg/kg while Group III was given L-ascorbic acid 40 mg/kg prior to Tolbutamide administration (20 mg/kg).
Administration:

Oral administration
Result:

L-ascorbic acid (40 mg/kg alone) produced 42.53% blood glucose reduction at 1.5 h and Tolbutamide 20 mg/kg produced 45.09 at 4 h. Administration of L-ascorbic acid 40 mg/kg body weight prior to Tolbutamide produced antidiabetic activity at 0.5 h and was maintained for 6 h.

Chemical Information

CAS No. 50-81-7
Appearance Solid
Molecular Weight 176.12
Formula C₆H₈O₆
Color White to off-white
SMILES

OC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O
Synonyms

L-Ascorbate; Vitamin C
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent	-80°C	1 year
	-20°C	6 months

Publications (81)

All 2D/3D Cell Culture and DifferentiationCell Proliferation/Viability AssayWBIHC

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Journal Impact Factor

Cancer Cell

PDGFRα+ITGA11+ fibroblasts foster early-stage cancer lymphovascular invasion and lymphatic metastasis via ITGA11-SELE interplay. [Abstract]

2024 Apr 8;42(4):682-700.e12. PMID: 38428409
Nat Immunol

Rebalancing TGFβ1/BMP signals in exhausted T cells unlocks responsiveness to immune checkpoint blockade therapy. [Abstract]

2023 Feb;24(2):280-294. PMID: 36543960
Adv Mater

Hydrolysis of 2D Nanosheets Reverses Rheumatoid Arthritis Through Anti-Inflammation and Osteogenesis. [Abstract]

2024 Dec 26:e2415543. PMID: 39726077
Mil Med Res

Direct conversion of human fibroblasts into dopaminergic neuron-like cells using small molecules and protein factors. [Abstract]

2020 Nov 1;7(1):52. PMID: 33129359
Cell Host Microbe

Roseburia intestinalis-derived butyrate alleviates neuropathic pain. [Abstract]

2024 Dec 12:S1931-3128(24)00444-X. PMID: 39706182
ACS Nano

Starfish-Inspired Synergistic Reinforced Hydrogel Wound Dressing: Dual Responsiveness and Enhanced Bioactive Compound Delivery for Advanced Skin Regeneration and Management. [Abstract]

2025 Mar 18;19(10):10180-10198. PMID: 40048360
Nat Commun

ATG8ylation-mediated tonoplast invagination mitigates vacuole damage. [Abstract]

2025 Jul 18;16(1):6621. PMID: 40681515
Bone Res

Enhanced SIRT3 expression restores mitochondrial quality control mechanism to reverse osteogenic impairment in type 2 diabetes mellitus. [Abstract]

2025 Mar 3;13(1):30. PMID: 40025004
J Adv Res

Antimycin A inhibits alpha-herpesvirus replication by disrupting the formation of pyrimidinosomes. [Abstract]

2025 May 10:S2090-1232(25)00309-1. PMID: 40354935
Biomaterials

Precision pore structure optimization of additive manufacturing porous tantalum scaffolds for bone regeneration: A proof-of-concept study. [Abstract]

2024 Aug 15:313:122756. PMID: 39182327

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Biomaterials. 2024 Aug 15:313:122756. [Abstract]

(A) Representative images of Alizarin Red staining on 14 and 21 days of BMSCs seeded on porous tantalum scaffolds and cultured in osteogenic induction medium containing 1 M β-glycerophosphate (β-Glycerophosphate disodium salt pentahydrate) and 10 mM ascorbic acid ( L-Ascorbic acid). (B) Quantitative analysis of dissolved calcium nodules after 14 days and 21 days (n = 4).
Redox Biol

Dissecting the process of human neutrophil lineage determination by using alpha-lipoic acid inducing neutrophil deficiency model. [Abstract]

2022 Aug;54:102392. PMID: 35797799
Mater Today Bio

A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis. [Abstract]

2025 Jun 21:33:102009. PMID: 40673132
J Neuroinflammation

Inhibition of Ephrin receptor signaling in the paraventricular nucleus attenuates psoriasis-like dermatitis via synaptic plasticity and immune homeostasis modulation. [Abstract]

2025 Sep 24;22(1):211. PMID: 40993793
Sci China Life Sci

Chemical conversion of human and mouse fibroblasts into motor neurons. [Abstract]

2018 Oct;61(10):1151-1167. PMID: 30159682
Acta Neuropathol

TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis. [Abstract]

2026 Mar 16;151(1):26. PMID: 41838122
Chin Herb Med

2026 Mar 1.
Virtual Phys Prototyp

2026 Mar 1;21.
Int J Biol Macromol

Trained immunity driven by Enterococcus faecalis ribosomal protein S11 enhances antigen presentation and boosts influenza vaccine efficacy via nanoparticle delivery. [Abstract]

2025 May 15:144179. PMID: 40381785
Int J Biol Macromol

Ligand "switching on" fluorescence of HIV-1 RNA-templated copper nanoclusters for ligand-RNA interaction assays. [Abstract]

2024 Jan;256(Pt 1):127779. PMID: 37981280
Free Radic Biol Med

Glsnf1-mediated metabolic rearrangement participates in coping with heat stress and influencing secondary metabolism in Ganoderma lucidum. [Abstract]

2020 Feb 1;147:220-230. PMID: 31883976
Mater Today Adv

2026 Feb 10.
Biomed Pharmacother

OAT3 mediates methotrexate resistance in the treatment of rheumatoid arthritis. [Abstract]

2022 Sep:153:113558. PMID: 36076621
Redox Rep

Inhibition of SLC40A1 represses osteoblast formation via inducing iron accumulation and activating the PERK/ATF4/CHOP pathway mediated oxidative stress. [Abstract]

2024 Dec;29(1):2428147. PMID: 39607819
Antioxidants (Basel)

Vitamin C Alleviates the Negative Effects of Heat Stress on Reproductive Processes by Regulating Amino Acid Metabolism in Granulosa Cells. [Abstract]

2024 May 27;13(6):653. PMID: 38929092

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 May 27;13(6):653. [Abstract]

L-Ascorbic acid (Vitamin C) (50-500 μM; 6 h) significantly increases the viability of heat-stressed gastric cancer cells at concentrations of 200 μM and 300 μM.
Ind Crops Prod

2025 Oct 18;237:122143.
J Agric Food Chem

Sodium Benzoate Inhibits Osteoblast Differentiation and Accelerates Bone Loss by Regulating the FGF2/p38/RUNX2 Pathway. [Abstract]

2025 Jun 4;73(22):13891-13901. PMID: 40404584
Antioxid Redox Signal

Cysteine Deprivation Targets Ovarian Clear Cell Carcinoma Via Oxidative Stress and Iron-Sulfur Cluster Biogenesis Deficit. [Abstract]

2020 Dec 10;33(17):1191-1208. PMID: 32336105

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Antioxid Redox Signal. 2020 Dec 10;33(17):1191-1208. [Abstract]

L-Ascorbic acid (Vitamin C) (250 μM, 1 h) significantly alleviates the growth suppression induced by combined SAS and PAG treatment only in OVISE cells, but shows no effect in KOC-7C cells.
Cancer Cell Int

Metabolomics reveals ascorbic acid inhibits ferroptosis in hepatocytes and boosts the effectiveness of anti-PD1 immunotherapy in hepatocellular carcinoma. [Abstract]

2024 May 31;24(1):192. PMID: 38822322

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 May 31;24(1):192. [Abstract]

L-Ascorbic acid (AA) (150 μL of 1.5 M; i.p.; every two days) enhances the expression of GPX4 and SLC7A11 proteins in mouse liver.

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 May 31;24(1):192. [Abstract]

L-Ascorbic acid (AA) (150ul of 1.5 M; i.p.; every two days) significantly decreases the expression of Ki67 in C57BL/6 mice.
Food Biosci

2025 Sep 18;73:107628.
Eur J Med Chem

Discovery of novel diphenylbutene derivative ferroptosis inhibitors as neuroprotective agents. [Abstract]

2022 Mar 5:231:114151. PMID: 35123296
Cell Biol Toxicol

CaMKII and CaV3.2 T-type calcium channel mediate Connexin-43-dependent inflammation by activating astrocytes in vincristine-induced neuropathic pain. [Abstract]

2023 Jun;39(3):679-702. PMID: 34286406

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2023 Jun;39(3):679-702. [Abstract]

L-Ascorbic acid (300 μM, 10 μL intrathecal injection) reduces the levels of CaV3.2, p-CaMKII, and Cx43 in mice.
J Ethnopharmacol

Serum metabolomics and 16S rRNA amplicon sequencing reveal the role of puerarin in alleviating bone loss aggravated by antidiabetic agent pioglitazone in type 2 diabetic mice. [Abstract]

2024 Nov 29:340:119128. PMID: 39617084
Food Funct

Vitamin C enhances the ex vivo proliferation of porcine muscle stem cells for cultured meat production. [Abstract]

2022 May 10;13(9):5089-5101. PMID: 35411884

L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Food Funct. 2022 May 10;13(9):5089-5101. [Abstract]

L-Ascorbic acid (Vitamin C) (100 μM; 10 min-4 h) upregulates the protein expression levels of IGF-1, p-IGF-1R, and SVCT2 in a time-dependent manner.
Int J Mol Sci

Synergistic Effects of a Novel Combination of Natural Compounds Prevent H2O2-Induced Oxidative Stress in Red Blood Cells. [Abstract]

2025 Feb 5;26(3):1334. PMID: 39941102
Bioorg Chem

Discovery of potent antiosteoporotic cyclic depsipeptides with an unusual nitrile hydroxy acid from Microascus croci. [Abstract]

2025 Jan 5:155:108133. PMID: 39798453
Bioorg Chem

Identifying eleven new ferroptosis inhibitors as neuroprotective agents from FDA-approved drugs. [Abstract]

2024 May:146:107261. PMID: 38460336
Bioorg Chem

A new ferroptosis inhibitor, isolated from Ajuga nipponensis, protects neuronal cells via activating NRF2-antioxidant response elements (AREs) pathway. [Abstract]

2021 Oct:115:105177. PMID: 34303035
Biosci Rep

Autologous decellularized extracellular matrix protects against H2O2-induced senescence and aging in adipose-derived stem cells and stimulates proliferation in vitro. [Abstract]

2019 May 21;39(5):BSR20182137. PMID: 31048361
Front Cell Dev Biol

Blockade of GCH1/BH4 Axis Activates Ferritinophagy to Mitigate the Resistance of Colorectal Cancer to Erastin-Induced Ferroptosis. [Abstract]

2022 Feb 10:10:810327. PMID: 35223839
Environ Toxicol Pharmacol

Assessment of the toxicity of acrolein on an alveolus microphysiological system. [Abstract]

2026 Mar:122:104954. PMID: 41644025
FASEB J

Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter. [Abstract]

2020 Sep;34(9):11474-11487. PMID: 32729950
J Cell Mol Med

Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability or immune function. [Abstract]

2020 Apr;24(7):4212-4222. PMID: 32119193
iScience

Modeling the precise interaction of glioblastoma with human brain region-specific organoids. [Abstract]

2024 Feb 5;27(3):109111. PMID: 38390494
J Cell Physiol

Dynamic changes of endothelial and stromal cilia are required for the maintenance of corneal homeostasis. [Abstract]

2024 May;239(5):e31215. PMID: 38308657
Hematol Oncol

TET2 regulates extranodal NK/T cell lymphoma progression through regulation of DNA methylation. [Abstract]

2024 Jul;42(4):e3295. PMID: 38979860
J Biol Chem

c-Src regulates δ-secretase activation and truncated Tau production by phosphorylating the E3 ligase Traf6. [Abstract]

2023 Dec;299(12):105462. PMID: 37977223
J Nat Prod

Ferroptosis Inhibitory Aromatic Abietane Diterpenoids from Ajuga decumbens and Structural Revision of Two 3,4-Epoxy Group-Containing Abietanes. [Abstract]

2022 Jul 22;85(7):1808-1815. PMID: 35796002
Mol Med Rep

Exendin‑4 promotes osteogenic differentiation of adipose‑derived stem cells and facilitates bone repair. [Abstract]

2019 Dec; 20(6): 4933–4942. PMID: 31661134
Cell Cycle

METTL3-mediated long non-coding RNA MIR99AHG methylation targets miR-4660 to promote bone marrow mesenchymal stem cell osteogenic differentiation. [Abstract]

2023 Feb;22(4):476-493. PMID: 36369887
J Neurosci Res

Enhanced mitochondrial inhibition by 3,4-dihydroxyphenyl-acetaldehyde (DOPAL)-oligomerized α-synuclein. [Abstract]

2019 Dec;97(12):1689-1705. PMID: 31420910
J Sex Med

Changes in Male Rat Sexual Behavior and Brain Activity Revealed by Functional Magnetic Resonance Imaging in Response to Chronic Mild Stress. [Abstract]

2018 Feb;15(2):136-147. PMID: 29275048
IUBMB Life

Bencaosome [16:0 Lyso PA+XLGB28-sRNA] improves osteoporosis by simultaneously promoting osteogenesis and inhibiting osteoclastogenesis in mice. [Abstract]

2024 Oct;76(10):832-844. PMID: 39012196
Environ Toxicol

Dichloroacetate attenuates the stemness of colorectal cancer cells via trigerring ferroptosis through sequestering iron in lysosomes. [Abstract]

2021 Apr;36(4):520-529. PMID: 33166055
Mol Pharmacol

LZ-106 inhibits anchorage-independent growth of non-small cell lung cancer cells by a positive feedback between reactive oxygen species induction and autophagy flux blockage. [Abstract]

2025 Nov;107(11):100078. PMID: 41110219
J Orthop Surg Res

RNA-sequencing-based elucidation of the mechanism underlying aFGF mediated regulation of BMSCs via the PI3K-AKT pathway and its implications for rotator cuff injury repair. [Abstract]

2025 Nov 14;20(1):997. PMID: 41239461
J Orthop Surg Res

Functional mechanism and clinical implications of miR-455-5p in delayed fracture healing. [Abstract]

2025 Oct 29;20(1):943. PMID: 41163026
J Orthop Surg Res

Investigation on the role of Icariin in tendon injury repair: focusing on tendon stem cell differentiation. [Abstract]

2025 Apr 15;20(1):379. PMID: 40234966
Genes (Basel)

CHIR99021 and Brdu Are Critical in Chicken iPSC Reprogramming via Small-Molecule Screening. [Abstract]

2024 Sep 13;15(9):1206. PMID: 39336797
Mol Biol Rep

ILK inhibition reduces osteophyte formation through suppression of osteogenesis in BMSCs via Akt/GSK-3β/β-catenin pathway. [Abstract]

2024 Mar 14;51(1):421. PMID: 38483756
Fitoterapia

Discovery of phloroglucinols from Hypericum japonicum as ferroptosis inhibitors. [Abstract]

2021 Sep:153:104984. PMID: 34216691
Clin Exp Pharmacol Physiol

AEBP1 restores osteoblastic differentiation under dexamethasone treatment by activating PI3K/AKT signalling. [Abstract]

2024 Nov;51(11):e13923. PMID: 39358837
J Mol Histol

KLF9 regulates osteogenic differentiation of mesenchymal stem cells. [Abstract]

2024 Aug;55(4):503-512. PMID: 38801643
Biochem Biophys Res Commun

Exploring the impact of high-altitude de-acclimatization on renal function: The roles of oxidative and endoplasmic reticulum stress in rat models. [Abstract]

2024 May 14:708:149770. PMID: 38518722
Drug Chem Toxicol

Amelioration of metabolic disorders in H9C2 cardiomyocytes induced by PM2.5 treated with vitamin C. [Abstract]

2024 May;47(3):347-355. PMID: 36815321
Anim Biotechnol

Vitamin C enhances the in vitro development of early porcine embryos by improving mitochondrial function. [Abstract]

2024 Nov;35(1):2404043. PMID: 39306701
J Pharmacol Toxicol Methods

Establishment of drug screening in human embryonic stem cells based on a high-content screening system. [Abstract]

Nov-Dec 2020;106:106913. PMID: 32822830
Biol Pharm Bull

Anti-osteoporosis Activity of Sodium Glucuronate in Preosteo-blast MC3T3-E1 Cells and an Ovariectomized Rat Model. [Abstract]

2025;48(6):895-907. PMID: 40571633
Cytotechnology

Substrate coated with autologous decellularized extracellular matrix facilitates in vitro spreading of spheroid from adipose-derived stem cells through regulating ERK1/2-MMP2/9 pathway. [Abstract]

2021 Dec;73(6):787-800. PMID: 34776629
Biol Methods Protoc

Optimizing drug sensitivity assays in patient-derived tumor organoids: a comparison of IC50 estimation methods and experimental parameters. [Abstract]

2025 Feb 13;10(1):bpaf012. PMID: 40060949
Pak J Pharm Sci

Syringaresinol inhibits ferroptosis and ameliorates glucocorticoid-induced MC3T3-E1 osteoporosis by modulating the Nrf2/SLC7A11/GPX4 pathway. [Abstract]

2026 Jan;39(1):102-116. PMID: 41482780
bioRxiv

2026 May 19.
SSRN

2026 Mar 20.
Res Sq

2025 Oct 27.
bioRxiv

2025 August 25.
Res Sq

2025 Jul 21.
SSRN

2025 Jan 14.
Med Rev (2021)

MST1 interactomes profiling across cell death in esophageal squamous cell carcinoma. [Abstract]

2024 Jun 14;4(6):531-543. PMID: 39664081
Research Square Print

2022 Aug.
Oxid Med Cell Longev

miR-154-5p Affects the TGF β 1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1. [Abstract]

2022 Jan 27:2022:7502632. PMID: 35126820
J Afr Ass Physiol Sci

7(1): 30-37, July 2019.
Nigerian Journal of Scientific Research

18 (3): 2019.

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (567.79 mM)

DMSO : 100 mg/mL (567.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.6779 mL

28.3897 mL

56.7795 mL

5 mM

1.1356 mL

5.6779 mL

11.3559 mL

10 mM

0.5678 mL

2.8390 mL

5.6779 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (28.39 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (28.39 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (28.39 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (567.79 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sebastian J Padayatty, et al. Vitamin C as an antioxidant: evaluation of its role in disease prevention. J Am Coll Nutr. 2003 Feb;22(1):18-35. [Content Brief]

[2]. Michael T Nelson, et al. Molecular mechanisms of subtype-specific inhibition of neuronal T-type calcium channels by ascorbate. J Neurosci. 2007 Nov 14;27(46):12577-83. [Content Brief]

[3]. Aleksander Hinek, et al. Sodium L-ascorbate enhances elastic fibers deposition by fibroblasts from normal and pathologic human skin. J Dermatol Sci. 2014 Sep;75(3):173-82. [Content Brief]

[4]. Sungrae Cho, et al. Hormetic dose response to L-ascorbic acid as an anti-cancer drug in colorectal cancer cell lines according to SVCT-2 expression. Sci Rep. 2018 Jul 27;8(1):11372. [Content Brief]

[5]. Satyanarayana Sreemantula, et al. Influence of antioxidant (L- ascorbic acid) on tolbutamide induced hypoglycaemia/antihyperglycaemia in normal and diabetic rats. BMC Endocr Disord. 2005 Mar 3;5(1):2. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	5.6779 mL	28.3897 mL	56.7795 mL	141.9487 mL
	5 mM	1.1356 mL	5.6779 mL	11.3559 mL	28.3897 mL
	10 mM	0.5678 mL	2.8390 mL	5.6779 mL	14.1949 mL
	15 mM	0.3785 mL	1.8926 mL	3.7853 mL	9.4632 mL
	20 mM	0.2839 mL	1.4195 mL	2.8390 mL	7.0974 mL
	25 mM	0.2271 mL	1.1356 mL	2.2712 mL	5.6779 mL
	30 mM	0.1893 mL	0.9463 mL	1.8926 mL	4.7316 mL
	40 mM	0.1419 mL	0.7097 mL	1.4195 mL	3.5487 mL
	50 mM	0.1136 mL	0.5678 mL	1.1356 mL	2.8390 mL
	60 mM	0.0946 mL	0.4732 mL	0.9463 mL	2.3658 mL
	80 mM	0.0710 mL	0.3549 mL	0.7097 mL	1.7744 mL
	100 mM	0.0568 mL	0.2839 mL	0.5678 mL	1.4195 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

L-Ascorbic acid

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L-Ascorbic acid

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