Fumigatin
Fumigatin has anti-Gram-positive bacteria, negative bacteria (weak) and vibrio cholerae activities.
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- CAS No.: 484-89-9
- Formule: C8H8O4
- Masse moléculaire:168.15
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
Chemical Information
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CAS No. 484-89-9
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Masse moléculaire 168.15
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Formule C8H8O4
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SMILES
O=C1C=C(C)C(C(O)=C1OC)=O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Pureté et documentation
Références
[1]. W K Anslow, et al. Studies in the biochemistry of micro-organisms: Fumigatin (3-hydroxy-4-methoxy-2:5-toluquinone), and spinulosin (3:6-dihydroxy-4-methoxy-2:5-toluquinone), metabolic products respectively of Aspergillus fumigatus Fresenius and Penicillium spinulosum Thom. Biochem J. 1938 Apr;32(4):687-96. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)