2. Cytoskeleton
  3. Gap Junction Protein
  4. Gap19 TFA

Gap19 TFA, a peptide derived from nine amino acids of the Cx43 cytoplasmic loop (CL), is a potent and selective connexin 43 (Cx43) hemichannel blocker. Gap19 TFA inhibits hemichannels caused by preventing intramolecular interactions of the C-terminus (CT) with the CL. Gap19 TFA is not blocking GJ channels or Cx40/pannexin-1 hemichannels. Gap19 TFA has protective effects against myocardial.

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Custom Peptide Synthesis

Gap19 TFA Chemical Structure

Gap19 TFA Chemical Structure

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1 mg USD 50 In-stock
5 mg USD 115 In-stock
10 mg USD 175 In-stock
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Based on 3 publication(s) in Google Scholar

Other Forms of Gap19 TFA:

Gap19 TFA Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Gap19 TFA, a peptide derived from nine amino acids of the Cx43 cytoplasmic loop (CL), is a potent and selective connexin 43 (Cx43) hemichannel blocker. Gap19 TFA inhibits hemichannels caused by preventing intramolecular interactions of the C-terminus (CT) with the CL. Gap19 TFA is not blocking GJ channels or Cx40/pannexin-1 hemichannels. Gap19 TFA has protective effects against myocardial[1][2].

IC50 & Target

Cx43 Hemichannel[1]
In Vitro

Gap19 TFA (250 μM; for 30 min ) decreases mitochondrial potassium uptake[1].
Gap19 TFA (400 μM) inhibits unitary hemichannel currents in HeLa-Cx43 cells[2].
Gap19 TFA (100 μM) inhibits hemichannel unitary currents in ventricular cardiomyocytes[2].
Gap19 TFA (250 μM, 30 min) protects against myocardial ischemia/reperfusion injury in vitro and in vivo[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Gap19 TFA Related Antibodies
In Vivo

Gap19 TFA (iv; 25 mg/kg; 10 min before ligation) significantly reduces the infarct size by approximately one-fifth[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57/BL6 mice[2]
Dosage: 25 mg/kg
Administration: IV; 10 min before ligation
Result: Significantly reduced the infarct size by approximately one-fifth.
Molecular Weight

1275.46

Formula

C57H97F3N14O15
Appearance

Solid

Color

White to off-white

Sequence Shortening

KQIEIKKFK
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (78.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (78.40 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7840 mL 3.9202 mL 7.8403 mL
5 mM 0.1568 mL 0.7840 mL 1.5681 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (78.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 0.7840 mL 3.9202 mL 7.8403 mL 19.6008 mL
5 mM 0.1568 mL 0.7840 mL 1.5681 mL 3.9202 mL
10 mM 0.0784 mL 0.3920 mL 0.7840 mL 1.9601 mL
15 mM 0.0523 mL 0.2613 mL 0.5227 mL 1.3067 mL
20 mM 0.0392 mL 0.1960 mL 0.3920 mL 0.9800 mL
25 mM 0.0314 mL 0.1568 mL 0.3136 mL 0.7840 mL
30 mM 0.0261 mL 0.1307 mL 0.2613 mL 0.6534 mL
40 mM 0.0196 mL 0.0980 mL 0.1960 mL 0.4900 mL
50 mM 0.0157 mL 0.0784 mL 0.1568 mL 0.3920 mL
60 mM 0.0131 mL 0.0653 mL 0.1307 mL 0.3267 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Gap19 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gap19Gap 19Gap-19Gap Junction ProteinCx43connexinhemichannelC-terminusCTcytoplasmicloopCLGJchannelsCx40/pannexin-1myocardialInhibitorinhibitorinhibit

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