country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-D2443

Copy Link Print Information

AF594 DBCO

AF594 DBCO is an AlexaFluor 594-conjugated DBCO click chemistry probe for fluorescent labeling of azido-modified cholesterol probes. DBCO is a commonly used chemical biomarker group. AF594 DBCO (Excitation wavelength about 590 nm, emission wavelength about 617 nm) can be used to label proteins, cells and other biomolecules for fluorescence imaging and flow cytometry detection.

For research use only. We do not sell to patients.

Formula: C₅₃H₄₈N₄O₁₁S₂
Molecular Weight:981.10

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (278 KB)

AF594 DBCO Chemical Structure

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

AF594 DBCO efficiently click-labels azido-functionalized cholesterol probe in fixed COS-7 cells, with fluorescence intensity varying inversely with FBS concentration in the cholesterol probe treatment medium^[1].
AF 594-DBCO enables nanoscale visualization of azido-functionalized cholesterol probe in COS-7 cells via TREx combined with Airyscan SR or SIM, resolving cholesterol aggregates as small as 36.5 nm and their colocalization with late endosomes/lysosomes^[1].
AF 594-DBCO enables tracking of azido-functionalized cholesterol probe localization in COS-7 cells via 4× ExM, showing time-dependent colocalization with LAMP1-positive late endosomes/lysosomes and minimal colocalization with clathrin-coated pits^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 981.10
Formula C₅₃H₄₈N₄O₁₁S₂
SMILES

C[N+]1=C2C(C(CS(=O)([O-])=O)=CC1(C)C)=CC3=C(C4=CC=CC=C4C(O)=O)C5=CC6=C(N(C)C(C)(C)C=C6CS(=O)(O)=O)C=C5OC3=C2.CC(NCCC(N7CC8=C(C=CC=C8)C#CC9=C7C=CC=C9)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Gang Wen, et al. A Multifunctional Probe for Visualization of the Nanoscale Distribution of Cholesterol in Cells by Expansion Microscopy. Aggregate, 2025; 6:e70104.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote