DAF-2DA
Based on 2 publication(s) in Google Scholar
DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 95.70%
- No. CAS: 205391-02-2
- Fòrmula: C24H18N2O7
- Peso molecular:446.41
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Almacenamiento:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Publications Citing Use of MedChemExpress (MCE) DAF-2DA
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Actividad biológica
Descripciòn
In Vitro
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
538
Excitation (Ex)
485
Chemical Information
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No. CAS 205391-02-2
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Appearance Solid
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Peso molecular 446.41
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Fòrmula C24H18N2O7
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Color Light yellow to yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(N)C(N)=C5
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Synonyms
5,6-Diaminofluorescein diacetat
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Metab
Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]2025 Jan 7;37(1):291-304.e9. PMID: 39413790 -
Cell Commun Signal
Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1. [Abstract]2025 Jan 13;23(1):21. PMID: 39800699
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DAF-2DA Related Classifications
HY-D0032 Related Classifications
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