Schisandrin C
Based on 5 publication(s) in Google Scholar
Schisandrin C (Schizandrin-C) is a phytochemical lignan isolated from Schizandra chinensis. Schisandrin C has diverse biological activities, including anticancer, anti-inflammatory and antioxidant effects. Schisandrin C can be used for cancer, alzheimer’s disease, and liver diseases research. Schisandrin C induces cell apoptosis.
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- Pureza : 99.94%
- No. CAS: 61301-33-5
- Fòrmula: C22H24O6
- Peso molecular:384.42
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
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Help & FAQs
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Pinoresinol Diglucoside (Standard)Analytical Standard
Publications Citing Use of MedChemExpress (MCE) Schisandrin C
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Flow Cytometry
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Histological Imaging/Staining
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IHC
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ELISA
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human A549 cells after 72 hrs by SRB assay
Antiproliferative activity against human A549 cells after 72 hrs by SRB assay
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[PMID: 18063366] |
| HCT-15 | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human HCT15 cells after 72 hrs by SRB assay
Antiproliferative activity against human HCT15 cells after 72 hrs by SRB assay
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[PMID: 18063366] |
| HeLa | IC50 |
>100 μM
Compound: 3
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Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
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[PMID: 23237974] |
| HepG2 | IC50 |
3.85 μM
Compound: 26
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Inhibition of PCSK9 mRNA expression in human HepG2 cells after 24 hrs by SYBR Green I dye-based qRT-PCR analysis
Inhibition of PCSK9 mRNA expression in human HepG2 cells after 24 hrs by SYBR Green I dye-based qRT-PCR analysis
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[PMID: 30448414] |
| HL-60 | IC50 |
41.2 μM
Compound: 8
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Cytotoxicity against human HL60 cells by XTT assay
Cytotoxicity against human HL60 cells by XTT assay
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[PMID: 16562834] |
| K562 | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
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[PMID: 18063366] |
| Lewis lung carcinoma cell line | IC50 |
>100 μM
Compound: 3
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Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
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[PMID: 23237974] |
| MDA-MB-231 | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by SRB assay
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[PMID: 18063366] |
| SK-HEP1 | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human SK-HEP1 cells after 72 hrs by SRB assay
Antiproliferative activity against human SK-HEP1 cells after 72 hrs by SRB assay
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[PMID: 18063366] |
| SMMC-7721 | IC50 |
>100 μM
Compound: 3
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Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay
Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay
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[PMID: 23237974] |
| SNU-638 | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human SNU638 cells after 72 hrs by SRB assay
Antiproliferative activity against human SNU638 cells after 72 hrs by SRB assay
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[PMID: 18063366] |
| T47D | IC50 |
>100 μM
Compound: Wuweizisu C
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Antiproliferative activity against human T47D cells after 72 hrs by SRB assay
Antiproliferative activity against human T47D cells after 72 hrs by SRB assay
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[PMID: 18063366] |
| U-87MG ATCC | IC50 |
>100 μM
Compound: 3
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Cytotoxicity against human U87 cells after 72 hrs by MTT assay
Cytotoxicity against human U87 cells after 72 hrs by MTT assay
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[PMID: 23237974] |
In Vitro
Schisandrin C (25-100 μM; 48 hours) down-regulates expression levels of Bcl-2, Bcl-xL and a concomitant degradation of poly(ADP-ribose) polymerase (PARP). It also activates caspase-3 and -9 in U937 cells[2].Schisandrin C (25-100 μM; 48 hours) induces apoptosis in a dose-dependent manner. And the fragmentation of genomic DNA is also increased in U937 cells[1].Schisandrin C (25-100 μM; 72 hours) induces G1 arrest, it down-regulates cyclin D1, cyclin E, cyclin-dependent kinase (Cdk) 4 and E2Fs expression, and also exhibits inhibition of pRB, and up-regulation of the Cdk inhibitor p21(WAF1/CIP1)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:U937 cells
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Concentration:25 μM,50 μM,100 μM
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Incubation Time:48 hours
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Result:Decreased the expression of apoptosis related proteins.
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Cell Line:U937 cells
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Concentration:25 μM,50 μM,100 μM
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Incubation Time:48 hours
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Result:Induced cell apoptosis.
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Cell Line:U937 cells
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Concentration:25 μM,50 μM,100 μM
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Incubation Time:48 hours
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Result:Induced growth inhibition and G1 arrest of U937 cells.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Aβ1-42-induced Alzheimer’s disease mice[3]
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Dosage:15-150 μg/kg
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Administration:Lateral ventricle injection (i.c.v.); 15-150 μg/kg; 5 days
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Result:Exhibited potent neuroprotective effects linking to anti-ChEtotal activities and anti-oxidative mechanisms in Aβ1-42-induced amnestic mice.
Chemical Information
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No. CAS 61301-33-5
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Appearance Solid
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Peso molecular 384.42
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Fòrmula C22H24O6
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Color White to off-white
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SMILES
[H][C@@]([C@](C)([H])C1)(C)CC2=CC3=C(C(OC)=C2C4=C1C=C5OCOC5=C4OC)OCO3
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Synonyms
Schizandrin-C; Wuweizisu-C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
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Chin Med
The combination of Schisandrin C and Luteolin synergistically attenuates hepatitis B virus infection via repressing HBV replication and promoting cGAS-STING pathway activation in macrophages. [Abstract]2024 Mar 18;19(1):48. PMID: 38500179 -
J Ethnopharmacol
2023 Jul 15:311:116427. PMID: 37001770 -
Eur J Pharmacol
Schizandrin A enhances the efficacy of gefitinib by suppressing IKKβ/NF-κB signaling in non-small cell lung cancer. [Abstract]2019 Jul 15:855:10-19. PMID: 31028739 -
Front Cell Dev Biol
Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress. [Abstract]2021 Nov 10;9:763864. PMID: 34858986
Schisandrin C purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Nov 10;9:763864. [Abstract]
The Cell viability of L02 cells treated with different concentrations of Schisandrin C (Sin C) (0, 5, 10, 20, 40, 60, 80, 100, 120 μM) and Curdione was determined using CCK-8 kit.
Schisandrin C purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Nov 10;9:763864. [Abstract]
L02 cells were treated with Schisandrin C (SinC) (10, 20, and 40 μM) for 18 h without any treatment and the percentage of ROS positive cells were detected with MitoSox staining and analyzed by FACS.
Schisandrin C purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Nov 10;9:763864. [Abstract]
The mice were pretreated with Schisandrin C (SinC) (200 mg/kg), Curdione or combination of SinC and Curdione vehicle by gavage and fed with MCD feed for 6 weeks. Histopathological changes in the liver.
Schisandrin C purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Nov 10;9:763864. [Abstract]
Immunohistochemical staining of α-SMA and collagen I pretreated with Schisandrin C (SinC) (200 mg/kg), Curdione or combination of SinC and Curdione vehicle.
Schisandrin C purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2021 Nov 10;9:763864. [Abstract]
The amount of glutathione (GSH) in liver of mice were measured using commercial ELISA kits treated with Schisandrin C (Sin C) (200 mg/kg).
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607
Solvente y solubilidad
In Vitro:
DMSO : 8.33 mg/mL (21.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution
This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Pureza y Documentación
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Ficha de datos (289 KB)
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SDS (537 KB)
- English - EN (537 KB)
- Français - FR (537 KB)
- Deutsch - DE (537 KB)
- Norwegian - NO (537 KB)
- Español - ES (537 KB)
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- Italian - IT (537 KB)
- Korean - KR (537 KB)
- Portuguese - PT (537 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Park C, et al. Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells. Int J Mol Med. 2009 Oct;24(4):495-502. [Content Brief]
[2]. Oh SY, et al. Anti-inflammatory effects of gomisin N, gomisin J, and schisandrin C isolated from the fruit of Schisandra chinensis. Biosci Biotechnol Biochem. 2010;74(2):285-91. [Content Brief]
[3]. Xin Mao, et al.Schisandrin C Ameliorates Learning and Memory Deficits by Aβ 1-42 -induced Oxidative Stress and Neurotoxicity in Mice. Phytother Res. 2015 Sep;29(9):1373-1380. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6013 mL | 13.0066 mL | 26.0132 mL | 65.0330 mL |
| 5 mM | 0.5203 mL | 2.6013 mL | 5.2026 mL | 13.0066 mL | |
| 10 mM | 0.2601 mL | 1.3007 mL | 2.6013 mL | 6.5033 mL | |
| 15 mM | 0.1734 mL | 0.8671 mL | 1.7342 mL | 4.3355 mL | |
| 20 mM | 0.1301 mL | 0.6503 mL | 1.3007 mL | 3.2517 mL |
Schisandrin C Related Classifications
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