Azedarachin B
Azedarachin B is a tetranortriterpenoid limonoid analog that is isolated and extracted from the root bark of Melia toosendan and the roots of Melia azedarach. Azedarachin B exhibits potent insecticidal activity and tumor cytotoxicity. Azedarachin B can be used in research on cancer and biopesticides.
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- No. CAS: 197457-99-1
- Fòrmula: C32H42O11
- Peso molecular:602.67
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB | IC50 |
4.9 nM
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Cytotoxicity against human KB cells.
Cytotoxicity against human KB cells.
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16880676 |
In Vitro
Azedarachin B shows highly potent lethality in the brine shrimp lethality test with an LC50 of 0.0098 µM[1].
Azedarachin B demonstrates potent cytotoxic activity against KB cells with an IC50 of 0.0049 µM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Spodoptera littoralis (Boisduval) (third-instar larvae)[2]
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Dosage:50, 100, 150, 200, 300, 400, 500 ppm
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Administration:Topical; leaf disk; 2-24 h
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Result:Exhibited antifeedant activity with a minimum inhibitory concentration of 200 ppm.
Chemical Information
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No. CAS 197457-99-1
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Peso molecular 602.67
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Fòrmula C32H42O11
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SMILES
C[C@]12[C@]34[C@]([C@H](C5=COC=C5)C[C@@]3([H])O4)([C@H](C([C@]1([H])[C@@]67[C@@]([C@@]([C@@H](OC6)OC(C(C)C)=O)([C@@H](C[C@@H]7O)OC(C)=O)C)([H])C[C@H]2O)=O)O)C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Azedarachin B Related Classifications
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