DBNBS
DBNBS is a radical scavenger. DBNBS undergoes non-radical nucleophilic addition to bind directly to cysteine (e.g., Cys-34 of BSA) to form a hydroxylamine, which is subsequently oxidized to produce an artifact spin adduct signal. DBNBS can be used for detecting biological free radicals.
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- No. CAS: 83016-63-1
- Fòrmula: C6H3Br2NO4S
- Peso molecular:344.97
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
DBNBS (2.5-5 mM; overnight) forms an artificial artifact nitroxide adduct with the Cys-34 thiol residue of BSA via non-radical nucleophilic addition followed by oxidation, whereas its tertiary carbon-centered radical adduct formed with photochemically oxidized BSA arises from genuine spin trapping[1].
DBNBS (2.5 mM; 1 min-2 h) rapidly and completely depletes the free Cys-34 thiol content of bovine serum albumin, with nearly complete depletion observable within 1 minute of incubation[1].
DBNBS (3.7 μM; 1 min-2 h) quenches the tryptophan fluorescence in bovine serum albumin through binding rather than chemical reactions, and exerts no effect on the fluorescence of free tryptophan under the tested conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 83016-63-1
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Peso molecular 344.97
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Fòrmula C6H3Br2NO4S
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SMILES
O=NC1=C(C=C(S(=O)(O)=O)C=C1Br)Br
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Silvester JA, et al. A study of photochemically-generated protein radical spin adducts on bovine serum albumin: the detection of genuine spin-trapping and artefactual, non-radical addition in the same molecule. Redox report : communications in free radical research. 1997 Aug;3(4):225-31. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DBNBS Related Classifications
HY-180497 Related Classifications
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