Diclofenac potassium
Based on 26 publication(s) in Google Scholar
Diclofenac potassium is a potent and nonselective anti-inflammatory agent, acts as a COX inhibitor, with IC50s of 4 and 1.3 nM for human COX-1 and COX-2 in CHO cells, and 5.1 and 0.84 μM for ovine COX-1 and COX-2, respectively. Diclofenac potassium induces apoptosis of neural stem cells (NSCs) via the activation of the caspase cascade.
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- Pureza : 99.85%
- No. CAS: 15307-81-0
- Fòrmula: C14H10Cl2KNO2
- Peso molecular:334.24
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Diclofenac potassium
More- Adv Funct Mater. 2026 Feb 3.
- J Hazard Mater. 2015 May 30:289:18-27. [Abstract]
- Biomater Res. 2025 Nov 7:29:0280. [Abstract]
- Int J Biol Macromol. 2026 Mar:352:151224. [Abstract]
- Emerg Contam. 2026 Feb 23.
- Cell Rep. 2025 Feb 7;44(2):115263. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119433. [Abstract]
- Gels. 2026 Jul 20;12(7):651.
- J Phys Chem Solids. 2017 October;109:117-123.
- Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]
- Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
- Chem Biol Interact. 2021 Apr 1:338:109425. [Abstract]
- ACS Infect Dis. 2024 Aug 9;10(8):2860-2869. [Abstract]
- Pharm Res. 2022 Oct;39(10):2515-2527. [Abstract]
- Biotechnol Bioeng. 2021 Dec;118(12):4687-4698. [Abstract]
- Drug Metab Dispos. 2026 Jul 14;54(8):100365.
- Arch Microbiol. 2024 Jun 7;206(7):289. [Abstract]
- J Pharm Biomed Anal. 2021 Jul 15:201:114105. [Abstract]
- Phys Chem Chem Phys. 2016 Jan 21;18(3):1526-36. [Abstract]
- Toxicol Mech Methods. 2019 Nov;29(9):654-664. [Abstract]
- Eur J Drug Metab Pharmacokinet. 2022 Sep;47(5):639-652. [Abstract]
- Thai Journal of Pharmaceutical Sciences. 2025 Dec.
- bioRxiv. 2024 Apr 7.
- Chemosphere. 2019 Jun:225:378-387. [Abstract]
- Federal University of Rio Grande do Sul. 2017 Nov.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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Flow Cytometry
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IF
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RT-PCR
Actividad biológica
Descripciòn
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Human COX-2 1.3 nM (IC50, in CHO cells) |
Human COX-1 4 nM (IC50, in CHO cells) |
Ovine COX-2 0.84 nM (IC50) |
Ovine COX-1 5.1 nM (IC50) |
In Vitro
Diclofenac effectively blocks COX-1 mediated prostanoid production from U937 cell microsomes, with an IC50 of 7±3 nM[1].
Diclofenac (1-60 μM; 1 day) induces neural stem cells (NSCs) death in a concentration-dependent manner[3].
Diclofenac (10-60 μM; 6 hours) increases the expression of cleaved (activated) caspase-3[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Neural stem cells (NSCs)
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Concentration:1, 3, 10, 30, 60 μM
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Incubation Time:1 day
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Result:Induction of cell death was concentration-dependent and the effect was not saturated at a concentration of up to 60 μM.
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Cell Line:Neural stem cells (NSCs)
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Concentration:10, 30 or 60 μM
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Incubation Time:6 hours
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Result:The activation of caspase-3 was increased in a concentration-dependent manner.
In Vivo
Diclofenac (10 mg/kg; administered via oral route just prior to induction of inflammation) shows in vivo anti-inflammatory activity in Wistar rats[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats (150±200 g)[1]
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Dosage:3 mg/kg
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Administration:Oral administration, b.i.d., for 5 days
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Result:Resulted in a significant increase in faecal 51Cr excretion.
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Animal Model:Wistar rats (150-175 g) bearing Formalin-induced rat foot paw edema model[2]
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Dosage:10 mg/kg
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Administration:Administered via oral route just prior to induction of inflammation
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Result:Showed in vivo anti-inflammatory activity (% edema inhibition=29.2, 1 h; 22.2, 3 h; 20, 6 h).
Chemical Information
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No. CAS 15307-81-0
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Appearance Solid
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Peso molecular 334.24
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Fòrmula C14H10Cl2KNO2
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Color White to off-white
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SMILES
O=C(O[K])CC1=CC=CC=C1NC2=C(Cl)C=CC=C2Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (26)
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Journal Impact Factor
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Most Recent
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Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
Cumulative release of DS (Diclofenac Sodium; 50 μg/mL; 0-24 h) from HDPCK hydrogels. DS showed rapid initial release, with approximately 80% released within half a day, followed by gradual release associated with hydrogel degradation.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
Intracellular ROS was visualized in chondrocytes stimulated with IL-1β and then co-cultured for 1 day with HDPC or HDPCK hydrogels containing DS (Diclofenac Sodium; 50 μg/mL); “+” groups received 808 nm NIR irradiation (0.75 W/cm²; 7 min). DS-containing hydrogels reduced intracellular ROS compared with the inflamed control group.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
Chondrocyte apoptosis was analyzed by flow cytometry after IL-1β-stimulated chondrocytes were co-cultured for 1 day with HDPC or HDPCK hydrogels containing DS (Diclofenac Sodium; 50 μg/mL). DS-containing hydrogels reduced both early and late apoptosis, with the strongest effects observed in the NIR-assisted groups.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
Immunofluorescence staining and semi-quantification of Col II, ACAN, and MMP13 were performed in IL-1β-stimulated chondrocytes after exposure to HDPC or HDPCK hydrogels containing DS (Diclofenac Sodium; 50 μg/mL; 1 day). DS-containing hydrogels restored Col II and ACAN expression while MMP13 was suppressed, with the most pronounced changes in the HDPCK+ group.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
RT-qPCR analysis of Col II, Acan, and Mmp13 mRNA expression was performed in IL-1β-stimulated chondrocytes after exposure to HDPC or HDPCK hydrogels containing DS (Diclofenac Sodium; 50 μg/mL; 1 day). Hydrogel exposure upregulated Col II and Acan and downregulated Mmp13, with the most significant changes in the HDPCK+ group.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
Western blotting was used to analyze proteins involved in inflammation-, cell metabolism-, and growth-related pathways in chondrocytes under IL-1β-induced inflammatory conditions following exposure to HDPCK or HDPCK+ hydrogels containing DS (Diclofenac Sodium; 50 μg/mL; 1 day).
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
H&E and Safranin O/Fast Green (SO/FG) staining of articular cartilage was performed in ACLT-induced osteoarthritis rats after intra-articular administration of HDPC or HDPCK hydrogels containing DS (Diclofenac Sodium; 50 μg/mL; i.a.; once every 2 weeks for 6 weeks). The results of H&E staining and SO/FG staining showed that the PBS group exhibited severe cartilage degeneration with significant loss of proteoglycans, whereas the treatment groups displayed effective cartilage protection.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2026 Feb 3.
Immunohistochemical staining and semi-quantification of Col II and ACAN were performed in ACLT-induced osteoarthritis rats after intra-articular administration of HDPC or HDPCK hydrogels containing DS (Diclofenac Sodium; 50 μg/mL; i.a.; once every 2 weeks for 6 weeks). After the incorporation of photothermal therapy and DS, articular cartilage was effectively protected.
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J Hazard Mater
Microwave-assisted activated carbon from cocoa shell as adsorbent for removal of sodium diclofenac and nimesulide from aqueous effluents. [Abstract]2015 May 30:289:18-27. PMID: 25702636 -
Biomater Res
Distinct Roles of Matrigel Enabled the Production of Expandable Hepatoblast and Polarized Hepatocyte Organoids from Human Embryonic Stem Cells under 3-Dimensional Suspension Conditions. [Abstract]2025 Nov 7:29:0280. PMID: 41209312 -
Int J Biol Macromol
Reticuline isomerase AKR1B1 with aldo-keto reductase activity and detoxification function from the insect Blaps rhynchopetera. [Abstract]2026 Mar:352:151224. PMID: 41791543 -
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Cell Rep
Spatial proteomics identifies a CRTC-dependent viral signaling pathway that stimulates production of interleukin-11. [Abstract]2025 Feb 7;44(2):115263. PMID: 39921859 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Ecotoxicol Environ Saf
PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]2025 Nov 15:307:119433. PMID: 41273832 -
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Cell Rep Methods
RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]2023 Oct 23;3(10):100599. PMID: 37797618 -
Int J Mol Sci
Diclofenac Sensitizes Signet Ring Cell Gastric Carcinoma Cells to Cisplatin by Activating Autophagy and Inhibition of Survival Signal Pathways. [Abstract]2022 Oct 11;23(20):12066. PMID: 36292923
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
KATOIII and KATO/DDP were treated with different concentration of Diclofenac (DCF) (0–1000 µM) for 48 h, and cell viability was assessed.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
The KATO/DDP cells were treated with Cisplatin alone or cisplatin in combination with Diclofenac (DCF) at 10 or 20 µM for 24 h, at 37 °C. The apoptosis cells were detected by using annexin V and PI staining and analyzed by flow cytometer.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
The combination effect of Diclofenac (DCF) (0, 5, 10, 20 μM) and Cisplatin on the expression of cleaved PARP in KATO/DDP cells was examined by Western blot analysis.
Diclofenac potassium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
The formation of autophagic vacuoles in KATO/DDP cells increased from 27% to 39% and 45% in cotreatments involving cisplatin and Diclofenac (DCF) at 10 and 20 μM, respectively, when compared with treatment of cisplatin alone.
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Chem Biol Interact
Regulatory effects of non-steroidal anti-inflammatory drugs on cardiac ion channels Nav1.5 and Kv11.1. [Abstract]2021 Apr 1:338:109425. PMID: 33617802 -
ACS Infect Dis
Diclofenac Sodium Restores the Sensitivity of Colistin-Resistant Gram-Negative Bacteria to Colistin. [Abstract]2024 Aug 9;10(8):2860-2869. PMID: 39073350 -
Pharm Res
Effect of Salt Form on Gelation and Drug Delivery Properties of Diclofenac-Loaded Poloxamer Gels for Delivery to Impaired Skin. [Abstract]2022 Oct;39(10):2515-2527. PMID: 36002613 -
Biotechnol Bioeng
An integrated biomimetic array chip for establishment of collagen-based 3D primary human hepatocyte model for prediction of clinical drug-induced liver injury. [Abstract]2021 Dec;118(12):4687-4698. PMID: 34478150 -
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Arch Microbiol
Diclofenac sodium effectively inhibits the biofilm formation of Staphylococcus epidermidis. [Abstract]2024 Jun 7;206(7):289. PMID: 38847838 -
J Pharm Biomed Anal
A validated UHPLC-MS/MS method for simultaneous determination of lumiracoxib and its hydroxylation and acyl glucuronidation metabolites in rat plasma: Application to a pharmacokinetic study. [Abstract]2021 Jul 15:201:114105. PMID: 33991809 -
Phys Chem Chem Phys
Adsorption of sodium diclofenac on graphene: a combined experimental and theoretical study. [Abstract]2016 Jan 21;18(3):1526-36. PMID: 26671178 -
Toxicol Mech Methods
Evaluation of 3D re-cellularized tissue engineering: a drug-induced hepatotoxicity model for hepatoprotectant research. [Abstract]2019 Nov;29(9):654-664. PMID: 31345115 -
Eur J Drug Metab Pharmacokinet
Evaluation of an Ussing Chamber System Equipped with Rat Intestinal Tissues to Predict Intestinal Absorption and Metabolism in Humans. [Abstract]2022 Sep;47(5):639-652. PMID: 35733077 -
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Chemosphere
Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]2019 Jun:225:378-387. PMID: 30884299 -
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (299.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 14.29 mg/mL (42.75 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Korean - KR (597 KB)
- Portuguese - PT (597 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Riendeau D, et al. Biochemical and pharmacological profile of a tetrasubstituted furanone as a highly selective COX-2 inhibitor. Br J Pharmacol. 1997 May;121(1):105-17. [Content Brief]
[2]. Labib MB, et al. Design, synthesis of novel isoindoline hybrids as COX-2 inhibitors: Anti-inflammatory, analgesic activities and docking study. Bioorg Chem. 2018 Oct;80:70-80. [Content Brief]
[3]. Chiho Kudo, et al. Diclofenac Inhibits Proliferation and Differentiation of Neural Stem Cells. Biochem Pharmacol. 2003 Jul 15;66(2):289-95. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.9919 mL | 14.9593 mL | 29.9186 mL | 74.7966 mL |
| 5 mM | 0.5984 mL | 2.9919 mL | 5.9837 mL | 14.9593 mL | |
| 10 mM | 0.2992 mL | 1.4959 mL | 2.9919 mL | 7.4797 mL | |
| 15 mM | 0.1995 mL | 0.9973 mL | 1.9946 mL | 4.9864 mL | |
| 20 mM | 0.1496 mL | 0.7480 mL | 1.4959 mL | 3.7398 mL | |
| 25 mM | 0.1197 mL | 0.5984 mL | 1.1967 mL | 2.9919 mL | |
| 30 mM | 0.0997 mL | 0.4986 mL | 0.9973 mL | 2.4932 mL | |
| 40 mM | 0.0748 mL | 0.3740 mL | 0.7480 mL | 1.8699 mL | |
| DMSO | 50 mM | 0.0598 mL | 0.2992 mL | 0.5984 mL | 1.4959 mL |
| 60 mM | 0.0499 mL | 0.2493 mL | 0.4986 mL | 1.2466 mL | |
| 80 mM | 0.0374 mL | 0.1870 mL | 0.3740 mL | 0.9350 mL | |
| 100 mM | 0.0299 mL | 0.1496 mL | 0.2992 mL | 0.7480 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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