Fomivirsen
Based on 2 publication(s) in Google Scholar
Fomivirsen (ISIS-2922) is an antisense phosphorothioate oligonucleotide. Fomivirsen is an antiviral reagent used in research on cytomegalovirus (CMV) retinitis. Fomivirsen binds to and degrades the mRNA of CMV immediate-early 2 protein, thereby inhibiting viral proliferation. Fomivirsen can be used in research related to cytomegalovirus retinitis and cytomegalovirus diseases.
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- Pureza: 99.47%
- No. CAS: 144245-52-3
- Fòrmula: C204H263N63O114P20S20
- Peso molecular:6682.40
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
- Pureza y Documentación
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Publications Citing Use of MedChemExpress (MCE) Fomivirsen
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Actividad biológica
Fomivirsen (0.1-10 μM; overnight) potently inhibits the replication of the HCMV strain AD169 in NHDF cells, with a mean EC50 of 0.37 μM[1].
Fomivirsen (1.2-10 μM; overnight) reduces the total infectious HCMV titer by 2 log units at a concentration of 2.2 μM, and reduces the intracellular titer by >3 log units at 10 μM, in NHDF cells[1].
Fomivirsen (0.1-1 μM; overnight pre-treatment) inhibits HCMV plaque formation in NHDF cells; for the AD169 strain, its IC70 is 0.1 μM, and for the Towne strain, its EC50 is 0.3 μM[1].
Fomivirsen (0.25-8 μM; 15-24 h) reduces the protein expression of HCMV IE1 and IE2 in NHDF cells in a dose-dependent manner[1].
Fomivirsen inhibits HCMV with an EC50 of 0.1 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NHDF cells infected with HCMV (MOI 0.5 PFU per cell)
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Concentration:0.25-8 μM
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Incubation Time:Overnight
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Result:Reduced steady-state levels of both 72 kDa and 86 kDa IE polypeptides in a dose-dependent manner.
Significantly reduced polypeptide levels at 0.5 μM.
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Cell Line:NHDF cells infected with HCMV (MOI 3 PFU per cell)
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Concentration:1 μM
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Incubation Time:15-20 h
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Result:Reduced the proportion of HCMV-infected cells exhibiting nuclear immunofluorescence to <10% (from >70% in untreated cells).
Reduced the intensity of fluorescence in positive cells.
| Species | Dose | Route | T1/2 |
|---|---|---|---|
| Rabbit[2] | 66 μg | i.v.t | 62 h |
Chemical Information
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No. CAS 144245-52-3
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Appearance Solid
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Peso molecular 6682.40
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Fòrmula C204H263N63O114P20S20
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Color White to off-white
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SMILES
[Fomivirsen]
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Synonyms
ISIS-2922
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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J Mass Spectrom
Characterisation of Antisense Oligonucleotides by Ion-Pair Reversed-Phase UHPLC-HRMS: Method development using Design of Experiments. [Abstract]2026 Apr;61(4):e70049. PMID: 41856548 -
Pureza y Documentación
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Ficha de datos (289 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2242 KB)
Referencias
[1]. Azad RF, et al. Antiviral activity of a phosphorothioate oligonucleotide complementary to RNA of the human cytomegalovirus major immediate-early region. Antimicrob Agents Chemother. 1993;37(9):1945-1954. [Content Brief]
[2]. Leeds JM, et al. Pharmacokinetics of a potential human cytomegalovirus therapeutic, a phosphorothioate oligonucleotide, after intravitreal injection in the rabbit. Drug Metab Dispos. 1997 Aug;25(8):921-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fomivirsen Related Classifications
HY-112958 Related Classifications
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