Gymnin
Gymnin is a defensin-like antifungal peptide. Gymnin has significant antifungal activities against phytopathogenic and mycotoxigenic fungi. Gymnin inhibits HIV-1 reverse transcriptase with an IC50 of 200 μM and the proliferation of tumor cells. Gymnin has a weak mitogenic activity toward murine splenocytes. Gymnin can be used for plant disease treatments research.
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- No. CAS: 1427515-26-1
- Fòrmula: C48H74N12O20S
- Peso molecular:1171.23
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Chemical Information
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No. CAS 1427515-26-1
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Peso molecular 1171.23
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Fòrmula C48H74N12O20S
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Sequence
Lys-Thr-Cys-Glu-Asn-Leu-Ala-Asp-Asp-Tyr
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Sequence Shortening
KTCENLADDY
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
Referencias
[1]. Leannec-Rialland V, et al. Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins. J Fungi (Basel). 2022 Feb 25;8(3):229. [Content Brief]
[2]. Wong JH, et al. Gymnin, a potent defensin-like antifungal peptide from the Yunnan bean (Gymnocladus chinensis Baill). Peptides. 2003 Jul;24(7):963-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Gymnin Related Classifications
HY-P11158 Related Classifications
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