Hadacidin
Based on 1 Customer Validation
Hadacidin is a competitive inhibitor of adenylate synthase, possessing antitumor, plant growth inhibitory, and other activities. Hadacidin competitively inhibits aspartate binding, blocking the formation of adenylosuccinate, the reamination of IMP, and de novo adenine nucleotide synthesis. Hadacidin inhibits mitosis and plant stem/root growth. Hadacidin induces mesenchymal/epithelial cell death at high concentrations. Hadacidin can be used in plant growth regulation and cancer-related research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 96.27%
- No. CAS: 689-13-4
- Fòrmula: C3H5NO4
- Peso molecular:119.08
-
Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
In Vitro
Hadacidin (2-20 mg/mL; 2-4 days) inhibits morphogenesis and mitosis in organ cultures of mandibular molar tooth germs of C57BL/6J mice; the effect is reversible after transfer to control medium; administration of adenine (0.05 mg/mL) or adenosine (0.1 mg/mL) partially prevents this effect[1].
Hadacidin competitively inhibits D. discoideum Ax-3 AMPS synthetase, producing approximately 50% inhibition at 100 μM and >95% inhibition at approximately 250 μM, with a Ki of 1.5 × 10-6 M[5].
Hadacidin competitively inhibits adenylosuccinate synthetase by competing with L-aspartate, blocks adenylosuccinate formation, and inhibits de novo biosynthesis of adenylate/deoxyadenylate in in vitro assays[6].
Hadacidin (5 mg/mL; 12-18 hours) reduces aspartate and thymidine uptake in D. discoideum Ax-3 by at least 75%; increases osmotic stability of D. discoideum Ax-3[5].
Hadacidin prevents the growth of D. discoideum Ax-3 in FM and HL-5, and growth resumes after washing out[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Hadacidin inhibits stem and root growth of Alaska pea plants grown in nutrient solution, and 16 days after foliar spraying, the average stem length decreases from 352 mm at 0 mg/L to 96 mm at 8000 mg/L[2].
Hadacidin inhibits the unfolding of the first trifoliolate leaf of pinto bean; when applied to the primary leaves of pinto bean, it is transported to the young trifoliolate leaves and inhibits their growth. In the bean leaf bioassay, the inhibition is counteracted by riboflavin, but not by glycine, whereas aspartic acid, asparagine, glutathione, and calcium pantothenate partially reverse it[2].
Hadacidin applied to tomato roots in nutrient solution completely stops root growth at 50 and 250 mg/L, and inhibits shoot growth by 40% at 50 mg/L and completely at 250 mg/L[2].
Hadacidin (100 mg/kg; i.v.; 30 min before stimulation) effectively blocks IMP (HY-108213) reamination in fast muscle of recovered rats; it also increases IMP accumulation in fast-twitch white gastrocnemius muscle, with no significant effect on IMP levels in fast-twitch red gastrocnemius muscle[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Sprague-Dawley (adult male, 302 g)[3]
-
Dosage:100 mg/kg, 50 mg/kg, i.v.
100 mg/kg, i.p. -
Administration:i.v., 30 min before stimulation; i.v.; i.p., one-half hour before stimulation
-
Result:Had no significant effect on IMP accumulation or on the level of any other measured metabolite during the 3 min of stimulation.
Had no effect on tension.
Inhibited the resynthesis of ATP from IMP by over 80% during the recovery period.
The same result was obtained with 50 mg/kg, i.v., or with 100 mg/kg, i.p., one-half hour before stimulation.
-
Animal Model:Sprague-Dawley (adult male, 363 g)[4]
-
Dosage:100 mg/kg i.p. at 45 min before exercise and 100 mg/kg i.p. at 15 min before exercise
-
Administration:i.p.; 2 equal doses at 45 min and 15 min before exercise
-
Result:Increased IMP accumulation in fast-twitch skeletal muscles of Sprague-Dawley rats after treadmill running, while not increasing IMP in slow-twitch soleus muscle.
Chemical Information
-
No. CAS 689-13-4
-
Appearance Solid
-
Peso molecular 119.08
-
Fòrmula C3H5NO4
-
Color Light yellow to light brown
-
SMILES
O=C(O)CN(O)C=O
-
Structure Classification
-
Initial Source
Escherichia coli
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
DMSO : 25 mg/mL (209.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (20.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (20.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (277 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 8.3977 mL | 41.9886 mL | 83.9772 mL | 209.9429 mL |
| 5 mM | 1.6795 mL | 8.3977 mL | 16.7954 mL | 41.9886 mL | |
| 10 mM | 0.8398 mL | 4.1989 mL | 8.3977 mL | 20.9943 mL | |
| 15 mM | 0.5598 mL | 2.7992 mL | 5.5985 mL | 13.9962 mL | |
| 20 mM | 0.4199 mL | 2.0994 mL | 4.1989 mL | 10.4971 mL | |
| 25 mM | 0.3359 mL | 1.6795 mL | 3.3591 mL | 8.3977 mL | |
| 30 mM | 0.2799 mL | 1.3996 mL | 2.7992 mL | 6.9981 mL | |
| 40 mM | 0.2099 mL | 1.0497 mL | 2.0994 mL | 5.2486 mL | |
| 50 mM | 0.1680 mL | 0.8398 mL | 1.6795 mL | 4.1989 mL | |
| 60 mM | 0.1400 mL | 0.6998 mL | 1.3996 mL | 3.4990 mL | |
| 80 mM | 0.1050 mL | 0.5249 mL | 1.0497 mL | 2.6243 mL | |
| 100 mM | 0.0840 mL | 0.4199 mL | 0.8398 mL | 2.0994 mL |
Hadacidin Related Classifications
HY-126055 Related Classifications
Powered by Bioz