MEGA-8
MEGA-8 (N-Octanoyl-N-methylglucamine) is a nonionic sugar-based surfactant. MEGA-8 can gently solubilize membrane proteins without easily causing their denaturation. MEGA-8 is a commonly used reagent for the extraction and study of membrane proteins in biochemistry.
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- Pureza : 99.33%
- No. CAS: 85316-98-9
- Fòrmula: C15H31NO6
- Peso molecular:321.41
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-W040217
MEGA-10Solubilizer -
HY-116285
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HY-W015993
Nonyl β-D-glucopyranosideSurfactant -
HY-W142692
Dodecyl β-D-glucopyranosideSurfactant -
HY-W127628
Decyl β-D-glucopyranosideGlucofuran -
HY-W040203
Decyl β-D-maltopyranosideEpithelial Permeability Enhancer -
HY-128974
N-Dodecyl-β-D-maltosideNonionic Detergent -
HY-W127785
N,N-Dimethyldodecylamine N-oxideBiochemical Assay Reagent -
HY-W040216
MEGA-9Non-ionic Surfactant -
HY-W019895
TetradecylphosphocholineDetergent -
HY-W040223
n-Heptyl β-D-thioglucosideBiochemical Assay Reagent -
HY-W127785R
N,N-Dimethyldodecylamine N-oxide (Standard)Biochemical Assay Reagent
Actividad biológica
Descripciòn
In Vitro
MEGA-8 can extract membrane-bound β-aryl ether lyase from Pseudomonas paucimobilis SYK-6 without disrupting its tertiary structure, and it can also detect the activity of β-aryl ether lyase in cell-free lysate[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 85316-98-9
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Appearance Solid
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Peso molecular 321.41
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Fòrmula C15H31NO6
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Color White to off-white
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SMILES
O[C@@H](CN(C)C(CCCCCCC)=O)[C@@H](O)[C@H](O)[C@H](O)CO
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Synonyms
N-Octanoyl-N-methylglucamine
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 175 mg/mL (544.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (155.56 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (268 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Masai E, et al. Detection and localization of a new enzyme catalyzing the beta-aryl ether cleavage in the soil bacterium (Pseudomonas paucimobilis SYK-6). FEBS Lett. 1989;249(2):348-352. [Content Brief]
[2]. Jeffrey GA, et al. The structures of 1-deoxy-(N-methyloctanamido)-D-glucitol (MEGA-8) and 1-deoxy-(N-methylundecanamido)-D-glucitol (MEGA-11). Acta Crystallogr B. 1989;45 ( Pt 4):447-452. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.1113 mL | 15.5565 mL | 31.1129 mL | 77.7823 mL |
| 5 mM | 0.6223 mL | 3.1113 mL | 6.2226 mL | 15.5565 mL | |
| 10 mM | 0.3111 mL | 1.5556 mL | 3.1113 mL | 7.7782 mL | |
| 15 mM | 0.2074 mL | 1.0371 mL | 2.0742 mL | 5.1855 mL | |
| 20 mM | 0.1556 mL | 0.7778 mL | 1.5556 mL | 3.8891 mL | |
| 25 mM | 0.1245 mL | 0.6223 mL | 1.2445 mL | 3.1113 mL | |
| 30 mM | 0.1037 mL | 0.5185 mL | 1.0371 mL | 2.5927 mL | |
| 40 mM | 0.0778 mL | 0.3889 mL | 0.7778 mL | 1.9446 mL | |
| 50 mM | 0.0622 mL | 0.3111 mL | 0.6223 mL | 1.5556 mL | |
| 60 mM | 0.0519 mL | 0.2593 mL | 0.5185 mL | 1.2964 mL | |
| 80 mM | 0.0389 mL | 0.1945 mL | 0.3889 mL | 0.9723 mL | |
| 100 mM | 0.0311 mL | 0.1556 mL | 0.3111 mL | 0.7778 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
MEGA-8 Related Classifications
HY-W040218 Related Classifications
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Keywords
- MEGA-8
- 85316-98-9
- N-Octanoyl-N-methylglucamine
- MEGA8
- MEGA 8
- Biochemical Assay Reagents
- glucitol chain
- cell-free lysates
- hydrogen-bond chain
- membrane protein crystallization
- orthorhombic crystals
- membrane-associated enzyme
- β-aryl ether cleaving enzyme
- β-hydroxypropiovanillone
- Pseudomonas paucimobilis SYK-6
- Inhibitor
- inhibitor
- inhibit