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Referencia número: HY-D1760

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Sodium-binding benzofuran isophthalate

SBFI is a cell-impermeant, fluorescent Na⁺ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm. SBFI selective for Na⁺ over K⁺ with K_d values of 20 and 120 mM for these ions, respectively. .

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No. CAS: 124549-08-2
Fòrmula: C₄₄H₄₂N₂O₁₅
Peso molecular:838.81

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Sodium-binding benzofuran isophthalate Chemical Structure

No. CAS: 124549-08-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Actividad biológica

Descripciòn

SBFI is a cell-impermeant, fluorescent Na⁺ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm^[1]. SBFI selective for Na⁺ over K⁺ with K_d values of 20 and 120 mM for these ions, respectively. ^[2].

In Vitro

The SBFI dye for each sample was prepared by : ^[1]
1. dissolving the appropriate quantity of dye powder in 3 mL of dimethyl sulphoxide.
2. The five solutions were agitated for 5 minutes.
3. Each sample was then exposed to ultraviolet light with a Leitz Orthoplan microscope, and fluorescence levels recorded.
4. Ten readings in total were achieved for each solution, agitating between readings.
5. For each set of sample results the highest, lowest, and median values were plotted against dye concentration.
6. From these tests the optimum fluorescence values, with minimum variation of upper and lower limits, was established with 50 g of SBFI dye.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

No. CAS 124549-08-2
Peso molecular 838.81
Fòrmula C₄₄H₄₂N₂O₁₅
SMILES

O=C(C1=CC=C(C(C(O)=O)=C1)C2=CC3=CC(OC)=C(N4CCOCCOCCN(CCOCC4)C5=C(OC)C=C6C=C(C7=CC=C(C(O)=O)C=C7C(O)=O)OC6=C5)C=C3O2)O
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Leeves MA, The effect of mechanical deformation on the distribution of ions in fibroblasts. Am J Orthod Dentofacial Orthop. 1995 Jun;107(6):625-32. [Content Brief]

[2]. Minta A, Fluorescent indicators for cytosolic sodium. J Biol Chem. 1989 Nov 15;264(32):19449-57. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Sodium-binding benzofuran isophthalate

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Sodium-binding benzofuran isophthalate

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Chemical Information

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Sodium-binding benzofuran isophthalate Related Classifications

HY-D1760 Related Classifications