CENPE - centromere protein E Gene

Also Known as KIF10; CENP-E; MCPH13; PPP1R61

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 1062

About CENPE

Cytogenetic location: 4q24 Genomic coordinates (GRCh38): 4:103,105,811-103,198,343 (from NCBI)

This gene has 6 transcripts (splice variants), 147 orthologues, 41 paralogues and is associated with 2 phenotypes. Broad expression in bone marrow (RPKM 3.4), lymph node (RPKM 3.3) and 18 other tissues.

Summary

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike Other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

CENPE Products (17)

mRNA	Protein	Name
XM_047449534.1	XP_047305490.1	centromere-associated protein E isoform X5
XM_011531549.3	XP_011529851.1	centromere-associated protein E isoform X10
XM_011531544.3	XP_011529846.1	centromere-associated protein E isoform X1
XM_047449541.1	XP_047305497.1	centromere-associated protein E isoform X15
XM_017007659.2	XP_016863148.1	centromere-associated protein E isoform X12
XM_047449538.1	XP_047305494.1	centromere-associated protein E isoform X11
NM_001286734.2	NP_001273663.1	centromere-associated protein E isoform 2
XM_011531547.3	XP_011529849.1	centromere-associated protein E isoform X6
XM_047449540.1	XP_047305496.1	centromere-associated protein E isoform X14
XM_011531545.3	XP_011529847.1	centromere-associated protein E isoform X2
XM_047449539.1	XP_047305495.1	centromere-associated protein E isoform X13
XM_011531548.3	XP_011529850.1	centromere-associated protein E isoform X7
XM_011531546.4	XP_011529848.1	centromere-associated protein E isoform X3
XM_047449535.1	XP_047305491.1	centromere-associated protein E isoform X8
XM_047449536.1	XP_047305492.1	centromere-associated protein E isoform X9
NM_001813.3	NP_001804.2	centromere-associated protein E isoform 1 precursor
XM_047449533.1	XP_047305489.1	centromere-associated protein E isoform X4

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables kinetochore binding	IDA IDA: Inferred from direct assay	9763420	GOA
enables microtubule binding	IDA IDA: Inferred from direct assay	25908662	GOA
enables microtubule motor activity	IDA IDA: Inferred from direct assay	25908662	GOA
enables microtubule motor activity	IMP IMP: Inferred from mutant phenotype	7889940	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	9763420	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in attachment of mitotic spindle microtubules to kinetochore	IMP IMP: Inferred from mutant phenotype	15843429	GOA
involved in chromosome segregation	IMP IMP: Inferred from mutant phenotype	24748105	GOA
involved in kinetochore assembly	IDA IDA: Inferred from direct assay	9763420	GOA
involved in lateral attachment of mitotic spindle microtubules to kinetochore	IMP IMP: Inferred from mutant phenotype	23891108	GOA
involved in metaphase chromosome alignment	IMP IMP: Inferred from mutant phenotype	23891108	GOA
involved in microtubule plus-end directed mitotic chromosome migration	IDA IDA: Inferred from direct assay	25908662	GOA
involved in mitotic cell cycle	IMP IMP: Inferred from mutant phenotype	24748105	GOA
involved in mitotic chromosome movement towards spindle pole	IDA IDA: Inferred from direct assay	25908662	GOA
involved in mitotic metaphase chromosome alignment	IMP IMP: Inferred from mutant phenotype	15843429	GOA
involved in mitotic spindle organization	IMP IMP: Inferred from mutant phenotype	25908662	GOA
involved in positive regulation of protein kinase activity	IMP IMP: Inferred from mutant phenotype	12925705	GOA
involved in regulation of mitotic metaphase/anaphase transition	IMP IMP: Inferred from mutant phenotype	2022189	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in chromosome	IDA IDA: Inferred from direct assay	2022189	GOA
located in chromosome, centromeric region	IDA IDA: Inferred from direct assay	2022189	GOA
located in condensed chromosome, centromeric region	IDA IDA: Inferred from direct assay	19465021	GOA
located in kinetochore	IDA IDA: Inferred from direct assay	9763420	GOA
located in kinetochore microtubule	IDA IDA: Inferred from direct assay	25908662	GOA
located in microtubule	IDA IDA: Inferred from direct assay	7889940	GOA
located in midbody	IDA IDA: Inferred from direct assay	2022189	GOA
located in mitotic spindle midzone	IDA IDA: Inferred from direct assay	2022189	GOA
located in nucleus	IMP IMP: Inferred from mutant phenotype	9763420	GOA
located in spindle midzone	IDA IDA: Inferred from direct assay	9763420	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

CENPE Protein Structure

Kinesin

0
500
1000
1500
2000
2500
2701 a.a.

Protein Preferred Names	Protein Names
centromere-associated protein E	Centromere autoantigen E (312kD) centromere protein E, 312kDa kinesin family member 10 kinesin-7 kinesin-related protein CENPE protein phosphatase 1, regulatory subunit 61

CENPE Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	CENPE	Q02224	BUB1B	Homo sapiens	O60566	Anti Bait CoIP	9763420
Intra	CENPE	Q02224	NUF2	Homo sapiens	Q9BZD4	Pull Down	17535814
Intra	CENPE	Q02224	NUF2	Homo sapiens	Q9BZD4	IF	17535814

Cross: Cross-species interaction Intra: Intraspecies interaction

CENPE Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P82738	CENPE Antibody (YA2483)	WB	Human
HY-P82738A	CENPE Antibody (YA2483)(PBS only)	WB	Human

Related Diseases

Diseases	Alias
Mosaic Variegated Aneuploidy Syndrome 1	Mva Syndrome MVA1 Mosaic Variegated Aneuploidy Syndrome, Type 1 Mosaic Variegated Aneuploidy Syndrome Congenital Chromosomal Disease
Microcephaly 13, Primary, Autosomal Recessive	MCPH13 Primary Autosomal Recessive Microcephaly 13 Microcephaly, Type 13, Primary, Autosomal Recessive
Congenital Nervous System Abnormality	Congenital Neurologic Anomaly Congenital Nervous System Disorder
Seckel Syndrome	Microcephalic Primordial Dwarfism Bird-Headed Dwarfism Harper'S Syndrome Virchow-Seckel Dwarfism Nanocephalic Dwarfism Sckl Seckel-Type Dwarfism
Primary Autosomal Recessive Microcephaly	Autosomal Recessive Primary Microcephaly Mcph True Microcephaly Microcephalia Vera Microcephaly Vera Microcephaly Primary Hereditary Microcephaly, Primary, Autosomal Recessive Primary Microcephaly
Fanconi Anemia, Complementation Group A	Fanconi Anemia Fanconi Pancytopenia Fanconi Anemia Complementation Group A FANCA Fa Fanconi Panmyelopathy Fanconi'S Anemia Fanconi Anaemia Fanconi'S Anaemia Fanconi Hypoplastic Anemia Estren-Dameshek Variant Of Fanconi Anemia Estren-Dameshek Variant Of Fanconi Pancytopenia Fanconi Anemia Estren-Dameshek Variant Fanconis Anemia
Microcephaly	Microencephaly Microcephalus Microcephalic Nanocephaly Congenital Microcephaly Brain Hypoplasia Brain Nondevelopment Cephalic Hypoplasia Undeveloped Cerebrum Undeveloped Brain Micrencephalon Micrencephaly
Alcohol Dependence	Alcoholism Alcohol Dependence, Susceptibility To Aerodigestive Tract Cancer, Squamous Cell, Alcohol-Related, Protection Against Alcohol Dependence, Protection Against Alcoholism, Susceptibility To Alcoholic Intoxication, Chronic Pharyngeal Neoplasms Chronic Alcoholism Dipsomania Alcohol Addiction Ethanol Dependence Chronic Ethanolism Chronic Alcoholic Disease Nos Alcoholic Disease Nos Alcoholic
Microcephaly 5, Primary, Autosomal Recessive	MCPH5 Primary Autosomal Recessive Microcephaly 5 Microcephaly Primary Autosomal Recessive 5 With Simplified Gyral Pattern Microcephaly, Primary Autosomal Recessive, 5
Primary Microcephaly	True Microcephaly Microcephaly, Primary
Mosaic Variegated Aneuploidy Syndrome	Warburton-Anyane-Yeboa Syndrome Mva Syndrome Mosaic Variegated Aneuplody Microcephaly Syndrome Warburton Anyane Yeboa Syndrome

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Clinical Phase
Pre-clinical Phase

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-10299	GSK-923295		99.95%	Phase 1

Pre-clinical Phase

Bioactive Molecules (9)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-19530	PF-2771	Inhibitor	99.66%	Unkown
HY-121569	Syntelin	Inhibitor	98.25%	Unkown
HY-RS02476	CENPE Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-150265	TNH	Activator	/	Unkown
HY-161897	CENP-E-IN-2	Inhibitor	/	Unkown
HY-161898	CENP-E-IN-3	Inhibitor	/	Unkown
HY-RS02477	Cenpe Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS02478	Cenpe Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-110090	UA62784		/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Bos taurus	CENPE	VGNC	VGNC:27175
Mus musculus	CENPE	MGD	MGI:1098230
Macaca mulatta	CENPE	VGNC	VGNC:71008
Felis catus	CENPE	VGNC	VGNC:60754
Canis familiaris	CENPE	VGNC	VGNC:39103
Rattus norvegicus	CENPE	RGD	RGD:1307115
Others	CENPE	NCBI