Gepotidacin mesylate hydrate
Based on 12 publication(s) in Google Scholar
Gepotidacin mesylate hydrate is an orally active triazaacenaphthylene antibiotic and bacterial type II topoisomerase inhibitor. Gepotidacin mesylate hydrate inhibits bacterial DNA replication by blocking topoisomerase enzymes. Gepotidacin mesylate hydrate selectively inhibits topoisomerase IV and the B subunit of DNA gyrase.
For research use only. We do not sell to patients.
- Purity: 99.62%
- CAS No.: 1624306-20-2
- Formula: C25H36N6O8S
- Molecular Weight:580.65
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Bioactive Compound Library Max
HY-L181
-
Bioactive Compound Library
HY-L001
-
Anti-Infection Compound Library
HY-L002
-
Cell Cycle/DNA Damage Compound Library
HY-L004
-
Anti-Aging Compound Library
HY-L034
-
Antibacterial Compound Library
HY-L049
-
Orally Active Compound Library
HY-L061
-
Antibiotics Library
HY-L067
-
Anti-Lung Cancer Compound Library
HY-L075
-
Highly Selective Inhibitors Library
HY-L158
-
MCE Bioactive Compound Library
HY-L001V
-
High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) Gepotidacin mesylate hydrate
More- Nat Microbiol. 2023 Mar;8(3):410-423. [Abstract]
- Cell Chem Biol. 2019 Sep 19;26(9):1274-1282.e4. [Abstract]
- PLoS Biol. 2020 Oct 5;18(10):e3000819. [Abstract]
- Antibiotics (Basel). 2022 Feb 1;11(2):192. [Abstract]
- ACS Infect Dis. 2026 Jan 9;12(1):363-375. [Abstract]
- ACS Infect Dis. 2024 Oct 11;10(10):3631-3639. [Abstract]
- Antimicrob Agents Chemother. 2026 Apr;70(4):e0170625. [Abstract]
- Antimicrob Agents Chemother. 2024 Dec 5;68(12):e0105124. [Abstract]
- Microbiol Spectr. 2022 Dec 21;10(6):e0205622. [Abstract]
- Vet Microbiol. 2023 Sep:284:109840. [Abstract]
- bioRxiv. 2026 Feb 23.
- University of Szeged. 2019 Jan.
Customer Validation & Images
-
Gel Electrophoresis
-
Microbiological Assay
-
Microbiological Assay
-
Microbiological Assay
-
Microbiological Assay
All Antibiotic Isoforms
MoreAll Topoisomerase Isoforms
More
Biological Activity
Gepotidacin is a potent inhibitor of gyrase-catalyzed DNA supercoiling (IC50 ≈ 0.047 μM) and relaxation of positively supercoiled substrates (IC50 ≈ 0.6 μM)[1].
The MIC of Gepotidacin for E. coli isolates in Mueller-Hinton broth ranged from 1 to 4 mg/L[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1624306-20-2
-
Appearance Solid
-
Molecular Weight 580.65
-
Formula C25H36N6O8S
-
Color Off-white to light yellow
-
SMILES
O=C(C=NC(C=C1)=C23)N3[C@H](CN4CCC(NCC5=CC(CCCO6)=C6C=N5)CC4)CN2C1=O.CS(=O)(O)=O.O.O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (12)
-
Journal Impact Factor
-
Most Recent
-
Nat Microbiol
Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains. [Abstract]2023 Mar;8(3):410-423. PMID: 36759752 -
Cell Chem Biol
Discovery of a Novel DNA Gyrase-Targeting Antibiotic through the Chemical Perturbation of Streptomyces venezuelae Sporulation. [Abstract]2019 Sep 19;26(9):1274-1282.e4. PMID: 31279606
Gepotidacin mesylate hydrate purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2019 Sep 19;26(9):1274-1282.e4. [Abstract]
Cross-Resistance of EN-7R Strains with Other Gyrase Inhibitors No observed cross-resistance between EN-7 and other gyrase-targeting antibiotics, ciprofloxacin and novobiocin; however, there is some cross-resistance to the NBTI Gepotidacin.
-
PLoS Biol
2020 Oct 5;18(10):e3000819. PMID: 33017402 -
Antibiotics (Basel)
Antibacterial Activity of the Novel Drug Gepotidacin against Stenotrophomonas maltophilia-An In Vitro and In Vivo Study. [Abstract]2022 Feb 1;11(2):192. PMID: 35203795
Gepotidacin mesylate hydrate purchased from MedChemExpress. Usage Cited in: Antibiotics (Basel). 2022 Feb 1;11(2):192. [Abstract]
MIC (mg/L) distribution of N = 100 Stenotrophomonas maltophilia strains for Gepotidacin as found in broth microdilution after EUCAST.
-
ACS Infect Dis
Activities of the Novel Bacterial Topoisomerase Inhibitor OSUAB-0284 against the Biothreat Pathogen Bacillus anthracis and Its Type II Topoisomerases. [Abstract]2026 Jan 9;12(1):363-375. PMID: 41430688
Gepotidacin mesylate hydrate purchased from MedChemExpress. Usage Cited in: ACS Infect Dis. 2026 Jan 9;12(1):363-375. [Abstract]
Gepotidacin induced primarily single-stranded DNA breaks mediated by B. anthracis gyrase.
-
ACS Infect Dis
Role of DNA Double-Strand Break Formation in Gyrase Inhibitor-Mediated Killing of Nonreplicating Persistent Mycobacterium tuberculosis in Caseum. [Abstract]2024 Oct 11;10(10):3631-3639. PMID: 39315541 -
Antimicrob Agents Chemother
Stepwise evolution and clonal enrichment of gepotidacin resistance in Neisseria gonorrhoeae. [Abstract]2026 Apr;70(4):e0170625. PMID: 41810969
Gepotidacin mesylate hydrate purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2026 Apr;70(4):e0170625. [Abstract]
In vitro induction of Gepotidacin (18-24 h) resistance in N. gonorrhoeae ST8123 occurred. MZX-24-16 (ST8123 isolate with GyrA S91F/D95A and ParC E91G) was used.
-
Antimicrob Agents Chemother
Multidrug tolerance conferred by loss-of-function mutations in anti-sigma factor RshA of Mycobacterium abscessus. [Abstract]2024 Dec 5;68(12):e0105124. PMID: 39470195 -
Microbiol Spectr
Dioxane-Linked Novel Bacterial Topoisomerase Inhibitors Exhibit Bactericidal Activity against Planktonic and Biofilm Staphylococcus aureus In Vitro. [Abstract]2022 Dec 21;10(6):e0205622. PMID: 36250857
Gepotidacin mesylate hydrate purchased from MedChemExpress. Usage Cited in: Microbiol Spectr. 2022 Dec 21;10(6):e0205622. [Abstract]
Time-kill kinetics of the novel bacterial topoisomerase inhibitor (NBTI) compound Gepotidacin and comparator agents for S. aureus were assessed.Untreated time (T) of 0 h, 1.67 × 106 CFU/mL; untreated T of 24 h, 3.66 × 109 CFU/mL.
-
Vet Microbiol
Antibacterial activity of novel bacterial topoisomerase inhibitors against key veterinary pathogens. [Abstract]2023 Sep:284:109840. PMID: 37531840 -
-
Solvent & Solubility
DMSO : 233.33 mg/mL (401.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Gibson EG, et al. Mechanistic and Structural Basis for the Actions of the Antibacterial Gepotidacin against Staphylococcus aureus Gyrase. ACS Infect Dis. 2019 Apr 12;5(4):570-581. [Content Brief]
[2]. Watkins RR, et al. Gepotidacin: a novel, oral, 'first-in-class' triazaacenaphthylene antibiotic for the treatment of uncomplicated urinary tract infections and urogenital gonorrhoea. J Antimicrob Chemother. 2023 May 3;78(5):1137-1142. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7222 mL | 8.6110 mL | 17.2221 mL | 43.0552 mL |
| 5 mM | 0.3444 mL | 1.7222 mL | 3.4444 mL | 8.6110 mL | |
| 10 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL | 4.3055 mL | |
| 15 mM | 0.1148 mL | 0.5741 mL | 1.1481 mL | 2.8703 mL | |
| 20 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1528 mL | |
| 25 mM | 0.0689 mL | 0.3444 mL | 0.6889 mL | 1.7222 mL | |
| 30 mM | 0.0574 mL | 0.2870 mL | 0.5741 mL | 1.4352 mL | |
| 40 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0764 mL | |
| 50 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8611 mL | |
| 60 mM | 0.0287 mL | 0.1435 mL | 0.2870 mL | 0.7176 mL | |
| 80 mM | 0.0215 mL | 0.1076 mL | 0.2153 mL | 0.5382 mL | |
| 100 mM | 0.0172 mL | 0.0861 mL | 0.1722 mL | 0.4306 mL |
Gepotidacin mesylate hydrate Related Classifications
HY-16742B Related Classifications
Powered by Bioz