Glycolyl-CoA
Glycolyl-CoA is a carboxyl acceptor substrate and a FORCE/TaCo pathway metabolite, can be generated by HACL-mediated condensation of formyl-CoA with formaldehyde, and can also be formed from glycolate via glycolyl-CoA synthetase, is carboxylated by GCC to tartronyl-CoA and supports the tartronyl-CoA photorespiration bypass for carbon fixation, and simultaneously can serve as an intermediate in HB-5.2-catalyzed oxidative condensation of ethylene glycol. Glycolyl-CoA can be used for research on metabolic pathways and carbon fixation mechanisms.
For research use only. We do not sell to patients.
- CAS No.: 1264-31-9
- Formula: C23H38N7O18P3S
- Molecular Weight:825.57
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Glycolyl-CoA (0.5 mM; preincubated at 37°C for 2 min) can be carboxylated by purified GCC M5, G20R, and L100N, among which G20R has the highest kcat (9.8 s−1) and GCC M5 has the lowest apparent KM (0.15 mM)[5].
Glycolyl-CoA (0.5 mM; preincubated at 37°C for 2 min) as substrate: L100N consumes 1.7 ATP per carboxylation, whereas GCC M5 consumes 4.0[5].
Glycolyl-CoA (injection volume 1 μL; flow rate 0.25 mL/min; column temperature 25°C) is quantified by LC-MS/MS in positive ion mode using the precursor ion 826.1 and product ions 428.1/319.1[5].
Glycolyl-CoA (4 mM; blotting 3.5 s) enables the cryo-EM structures of GCC M5 G20R and L100N at resolutions of 2.05 and 2.31 Å, respectively[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1264-31-9
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Molecular Weight 825.57
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Formula C23H38N7O18P3S
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SMILES
O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(OP(OCC(C)(C)[C@@H](O)C(NCCC(NCCSC(CO)=O)=O)=O)(O)=O)(O)=O)O[C@H]1N2C=NC3=C(N)N=CN=C32
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[2]. Orrego AH, et al. A Multi-Functional Heterogeneous Biocatalyst for the Oxygen-Free Oxidative Condensation of Primary Alcohols into β‑Hydroxy Acids. ACS catalysis. 2026 Jan 16;16(2):1192-1210. [Content Brief]
[3]. Yin X, et al. Extending the Farquhar-von Caemmerer-Berry photosynthesis model to account for various photorespiratory bypasses. The New phytologist. 2026 Nov;252(3):1327-1342. [Content Brief]
[5]. Marchal DG, et al. Machine Learning-Supported Enzyme Engineering toward Improved CO-Fixation of Glycolyl-CoA Carboxylase. ACS synthetic biology. 2023 Dec 15;12(12):3521-3530. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Glycolyl-CoA Related Classifications
HY-CE01812 Related Classifications
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