H2S Fluorescent probe 1

Cat. No.: HY-D2476 Purity: 98.10%: Data Sheet
COA

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Handling Instructions
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H2S Fluorescent probe 1 (Compound 2) is a fluorescent probe that detects hydrogen sulfide (H₂S) with almost no cytotoxicity. Upon the addition of increasing amounts of HS^- to DMSO solution of H2S Fluorescent probe 1, a new absorption peak appears gradually at 485 nm. H2S Fluorescent probe 1, the fluorescence intensity notes at 434 nm increasing rapidly by titration of HS^-.

For research use only. We do not sell to patients.

H2S Fluorescent probe 1 Chemical Structure

CAS No. : 433254-36-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Purity & Documentation
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Description

Molecular Weight

365.26

Formula

C₁₇H₁₀F₃NO₅

CAS No.

433254-36-5

Appearance

Solid

Color

White to off-white

SMILES

O=C1OC2=C(C(C)=C1)C=CC(OC3=CC=C([N+]([O-])=O)C=C3C(F)(F)F)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 30 mg/mL (82.13 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7378 mL	13.6889 mL	27.3778 mL
5 mM	0.5476 mL	2.7378 mL	5.4756 mL
10 mM	0.2738 mL	1.3689 mL	2.7378 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.9%

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Data Sheet (269 KB) SDS (252 KB)

COA (248 KB) HNMR (89 KB) RP-HPLC (77 KB) MS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Chen Y, et al. The synthesis, crystal, hydrogen sulfide detection and cell assement of novel chemsensors based on coumarin derivatives. Sci Rep. 2018 Nov 1;8(1):16159. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7378 mL	13.6889 mL	27.3778 mL	68.4444 mL
	5 mM	0.5476 mL	2.7378 mL	5.4756 mL	13.6889 mL
	10 mM	0.2738 mL	1.3689 mL	2.7378 mL	6.8444 mL
	15 mM	0.1825 mL	0.9126 mL	1.8252 mL	4.5630 mL
	20 mM	0.1369 mL	0.6844 mL	1.3689 mL	3.4222 mL
	25 mM	0.1095 mL	0.5476 mL	1.0951 mL	2.7378 mL
	30 mM	0.0913 mL	0.4563 mL	0.9126 mL	2.2815 mL
	40 mM	0.0684 mL	0.3422 mL	0.6844 mL	1.7111 mL
	50 mM	0.0548 mL	0.2738 mL	0.5476 mL	1.3689 mL
	60 mM	0.0456 mL	0.2281 mL	0.4563 mL	1.1407 mL
	80 mM	0.0342 mL	0.1711 mL	0.3422 mL	0.8556 mL